The New Age of Molecular Diagnostics for Microbial Agents

Whitley, Richard

doi:10.1056/nejmp0708085

Cited by 30 publications

(25 citation statements)

References 4 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Nevertheless, infection by as yet unidentified microorganisms cannot be ruled out, emphasizing the need for novel microbiological techniques. 34,35 The morphology of the granulomas in our patients was polymorphic and variable. Immunohistochemical studies showed a predominance of CD8 1 T cells, although the cell populations fluctuated over time.…”

Section: Discussionmentioning

confidence: 83%

Granulomatous inflammation in cartilage-hair hypoplasia: Risks and benefits of anti–TNF-α mAbs

Moshous

Meyts²,

Fraïtag

et al. 2011

Journal of Allergy and Clinical Immunology

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 83%

Granulomatous inflammation in cartilage-hair hypoplasia: Risks and benefits of anti–TNF-α mAbs

Moshous

Meyts²,

Fraïtag

et al. 2011

Journal of Allergy and Clinical Immunology

View full text Add to dashboard Cite

“…Until recently, the majority of surveillance and pathogen discovery efforts have relied heavily upon prior knowledge of the nucleotide sequence of known agents [114]. Prior techniques have amplified target sequences using known primer sequences with competitive PCR and subsequent microarray analysis, which then directs the search for a specific agent in question [115].…”

Section: Pathogen Discovery In Msmentioning

confidence: 99%

“…Prior techniques have amplified target sequences using known primer sequences with competitive PCR and subsequent microarray analysis, which then directs the search for a specific agent in question [115]. This strategy adopts an obvious level of bias when looking for pathogens as the causative agents for disease and fails to identify new pathogens and those that have diverged significantly from their related ancestors due to the lack of annealing of the specific primer sequence [114]. …”

Section: Pathogen Discovery In Msmentioning

confidence: 99%

Viruses and Multiple Sclerosis

Virtanen¹,

Jacobson²

2012

CNSNDDT

139

View full text Add to dashboard Cite

Multiple sclerosis (MS) is a heterogeneous disease that develops as an interplay between the immune system and environmental stimuli in genetically susceptible individuals. There is increasing evidence that viruses may play a role in MS pathogenesis acting as these environmental triggers. However, it is not known if any single virus is causal, or rather several viruses can act as triggers in disease development. Here, we review the association of different viruses to MS with an emphasis on two herpesviruses, Epstein-Barr virus (EBV) and human herpesvirus 6 (HHV-6). These two agents have generated the most impact during recent years as possible co-factors in MS disease development. The strongest argument for association of EBV with MS comes from the link between symptomatic infectious mononucleosis and MS and from seroepidemiological studies. In contrast to EBV, HHV-6 has been found significantly more often in MS plaques than in MS normal appearing white matter or non-MS brains and HHV-6 re-activation has been reported during MS clinical relapses. In this review we also suggest new strategies, including the development of new infectious animal models of MS and antiviral MS clinical trials, to elucidate roles of different viruses in the pathogenesis of this disease. Furthermore, we introduce the idea of using unbiased sequence-independent pathogen discovery methodologies, such as next generation sequencing, to study MS brain tissue or body fluids for detection of known viral sequences or potential novel viral agents.

show abstract

“…Newly developed techniques, such as pyrosequencing and microarrays (Whitley, 2008), automate this process and have demonstrated that the human microbiome is incredibly complex, with only a minority of bacterial organisms in the environment and human host identifiable by culture. Genomic techniques have the capacity to overcome the bacterial sampling problems that are often encountered in chronic wounds or in other settings in which multiple species coexist.…”

Section: Microbial Genomicsmentioning

confidence: 99%

Molecular Microbiology: New Dimensions for Cutaneous Biology and Wound Healing

Martin

Zenilman

Lazarus

2010

Journal of Investigative Dermatology

139

View full text Add to dashboard Cite

The role of bacteria in the pathogenesis of chronic, nonhealing wounds is unclear. All wounds are colonized with bacteria, but differentiating colonizers from invading organisms is difficult, if not impossible, at the present time. Furthermore, robust new molecular genomic techniques have shown that only 1% of bacteria can be grown in culture; anaerobes are especially difficult to identify using standard culture methods. Recent studies utilizing microbial genomic methods have demonstrated that chronic wounds are host to a wide range of microorganisms. New techniques also show that microorganisms are capable of forming highly organized biofilms within the wound that differ dramatically in gene expression and phenotype from bacteria that are typically seen in planktonic conditions. The aim of this review is to present a concise description of infectious agents as defined by new molecular techniques and to summarize what is known about the microbiology of chronic wounds in order to relate them to the pathophysiology and therapy of chronic wounds.

show abstract

The New Age of Molecular Diagnostics for Microbial Agents

Cited by 30 publications

References 4 publications

Granulomatous inflammation in cartilage-hair hypoplasia: Risks and benefits of anti–TNF-α mAbs

Granulomatous inflammation in cartilage-hair hypoplasia: Risks and benefits of anti–TNF-α mAbs

Viruses and Multiple Sclerosis

Molecular Microbiology: New Dimensions for Cutaneous Biology and Wound Healing

Contact Info

Product

Resources

About