The Neuropeptide-Related HERC5/TAC1 Interactions May Be Associated with the Dysregulation of lncRNA GAS5 Expression in Gestational Diabetes Mellitus Exosomes

Abstract

Objective. The goal of this work was to look at the expression and probable role of exosomal long noncoding RNA (lncRNA) GAS5 in gestational diabetes mellitus (GDM), as well as forecast the importance of its interaction with neuropeptides in the progression of the disease. Methods. We divided 44 pregnant women visiting the obstetric outpatient clinics at the Affiliated Hospital of Guilin Medical College from January 2021 to December 2021 into healthy and GDM groups. We measured the expression levels of the lncRNA GAS5 in peripheral blood using PCR and compared the expression levels between the 2 groups. The Gene Expression Omnibus (GEO) database and the R software were used to analyse the differences in the genes expressed in the amniotic fluid cells in the GDM and normal groups. catRAPID was used to identify potential target proteins for GAS5. Key neuropeptide-related proteins and potential target proteins of GAS5 were extracted, and protein interaction networks were mapped. AlphaFold 2 was used to predict the structure of the target protein. The ClusPro tool was used to predict protein-protein interactions. ZDOCK was used to further confirm the protein–nucleic acid docking. Results. The lncRNA GAS5 was downregulated in the peripheral blood of pregnant women with GDM compared with normal pregnant women. The subcellular localization sites of GAS5 were the nucleus, cytoplasm, and ribosome; in addition, GAS5 was present in exosomes. Intercellular interactions, including neuropeptide receptors, were increased in the amniotic fluid cells of patients with GDM. Venn diagram analysis yielded seven neuropeptide-related proteins and three GAS5 target proteins. Among them, HERC5/TAC1 interacted and GAS5 docked well with HERC5. Conclusion. The lncRNA GAS5 in the peripheral blood exosomes in patients with GDM may be a new target for the detection of GDM, and the interaction between GAS5 and HERC5/TAC1 may be involved in the pathogenesis of GDM.

Susceptibility Loci for Type 2 Diabetes in the Ethnically Endogamous Indian Sindhi Population: A Pooled Blood Genome-Wide Association Study

“…Published evidence implicated these 18 genes directly through population studies (GBP6, GSTO1, HHIPL1, INTS10, RGS16 and RNU6-679P), indirectly through association with other metabolic conditions (CCDC69, DNAH2, GALNT17, HERC5, KIF5C, MARCH2 and PRDM14) or through association with complications of T2D (CASC15, CCDC107, MIR147A, PFKFB3 and RNF166). Detailed annotation of the published literature in this regard is provided in Supplementary Table S3 ) [ 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 , 35 , 36 , 37 , 38 , 39 , 40 , 41 ]. From the remaining genes identified to be significantly associated with T2D in this study, there were two open reading frames, three long intergenic non-coding elements and a pseudogene.…”

Quercetin targets VCAM1 to prevent diabetic cerebrovascular endothelial cell injury

“…The corresponding structure information of the protein was obtained from Uniport, 3 get the entry of P19320 and download the corresponding Alphafold2 protein structure file as previous studies ( Burley et al, 2019 ; Tunyasuvunakool et al, 2021 ; Kang et al, 2022 ; Lu et al, 2022 ; Tang G.-Y. et al, 2022 ).…”

High-fiber-diet-related metabolites improve neurodegenerative symptoms in patients with obesity with diabetes mellitus by modulating the hippocampal–hypothalamic endocrine axis

“…The number of individuals with obesity is highest in China (1,2). Obesity and type 2 diabetes mellitus (T2DM) significantly increase the incidence of neurodegenerative diseases such as depression, dementia, stroke, and memory loss (3)(4)(5)(6)(7)(8). Chronic systemic inflammation throughout the body is a common feature of obesity and diabetes and may be present in the central nervous system, suggesting an important relationship among obesity, diabetes, and neurodegenerative diseases (9).…”