The neurons of class ALD mediate thermotaxis in the parasitic nematode, Strongyloides stercoralis

Lopez, P. Mark; Boston, Ray; Ashton, F.; Schad, Gerhard A.

doi:10.1016/s0020-7519(00)00087-4

Cited by 52 publications

(60 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Discussion S. stercoralis infective larvae respond positively to several physicochemical attractants. They are attracted to warmth, showing a strong response to temperatures in the range of avian and mammalian body temperature (9). They also respond positively to 3.3-4% carbon dioxide (13) and to sodium chloride (10), both nonspecific attractants produced by terrestrial vertebrates.…”

Section: Resultsmentioning

confidence: 99%

Urocanic acid is a major chemoattractant for the skin-penetrating parasitic nematode Strongyloides stercoralis

Safer¹,

Brenes

Dunipace

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Host-seeking behavior by parasitic nematodes relies heavily on chemical cues emanating from potential hosts. Nonspecific cues for Strongyloides stercoralis, a nematode that infects humans and a few other mammals, include carbon dioxide and sodium chloride; however, the characteristic species specificity of this parasite suggested the existence of other, more specific cues. Here we show that the infective larva of S. stercoralis is strongly attracted to an extract of mammalian skin and that the active component in this skin extract is urocanic acid. Urocanic acid, a histidine metabolite, is particularly abundant in mammalian skin and skin secretions, suggesting that it serves as an attractant specific to mammalian hosts. The attractant activity of urocanic acid is suppressed by divalent metal ions, suggesting a possible strategy for preventing infection.histidine ͉ host-seeking ͉ chemotaxis ͉ topical preventative

show abstract

Section: Resultsmentioning

confidence: 99%

Urocanic acid is a major chemoattractant for the skin-penetrating parasitic nematode Strongyloides stercoralis

Safer¹,

Brenes

Dunipace

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…C. elegans gpa-3 is expressed in eight pairs of chemosensory neurons in the amphidial complex (ADF, ADL, ASE, ASG, ASH, ASI, ASJ and ASK), two pairs of sensory neurons in the phasmids (PHA and PHB) and two other non-sensory cells (Jansen, et al, 1999). The amphidial neurons of S. stercoralis have been described and their homologies with amphidial neurons of C. elegans have been proposed based on morphology, position of cell bodies within the lateral ganglion and, in several cases, function inferred from microlaser ablation studies (Ashton, et al, 1995;Ashton, et al, 1998;Lopez, et al, 2000;Nolan, et al, 2004;Ashton, et al, 2007). We have not yet determined the specific identities of the GFP-expressing neurons in S. stercoralis transformed with pAJ09.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, transformation constructs that are expressed in such small, defined groupings of cells in S. stercoralis may provide the basis for genetically targeted cell ablation using modified channel forming proteins as described for C. elegans (Driscoll and Chalfie, 1991;Harbinder, et al, 1997). In some instances such an approach could provide an alternative to microlaser surgical techniques currently in use for S. stercoralis (Lopez, et al, 2000;Nolan, et al, 2004;Ashton, et al, 2007).…”

Section: Discussionmentioning

confidence: 99%

Strongyloides stercoralis: Cell- and tissue-specific transgene expression and co-transformation with vector constructs incorporating a common multifunctional 3′ UTR

Junio

Massey

et al. 2008

Experimental Parasitology

116

View full text Add to dashboard Cite

Transgenesis is a valuable methodology for studying gene expression patterns and gene function. It has recently become available for research on some parasitic nematodes, including Strongyloides stercoralis. Previously, we described a vector construct, comprising the promoter and 3′ UTR of the S. stercoralis gene Ss era-1 that gives expression of GFP in intestinal cells of developing F1 progeny. In the present study, we identified three new S. stercoralis promoters, which, in combination with the Ss era-1 3′ UTR, can drive expression of GFP or the red fluorescent protein, mRFPmars, in tissuespecific fashion. These include Ss act-2, which drives expression in body wall muscle cells, Ss gpa-3, which drives expression in amphidial and phasmidial neurons and Ss rps-21, which drives ubiquitous expression in F1 transformants and in the gonads of microinjected P0 female worms. Concomitant microinjection of vectors containing GFP and mRFPmars gave dually transformed F1 progeny, suggesting that these constructs could be used as co-injection markers for other transgenes of interest. We have developed a vector "toolkit" for S. stercoralis including constructs with the Ss era-1 3′ UTR and each of the promoters described above.

show abstract

“…L3i of S. stercoralis and many parasitic nematodes are developmentally arrested, nonfeeding, and resistant to extreme temperatures and desiccation. They are morphologically similar to the dauer larvae formed by free-living nematodes under unfavorable environmental conditions, a stage that has been extensively studied in C. elegans (Hawdon and Schad 1991;Lopez et al 2000). C. elegans dauers (L3d) can arrest for months, molting to L4 when favorable conditions return, and much is known about the molecular genetic control of dauer entry and exit (Riddle and Albert 1997).…”

Section: Strongyloides Pathogenesis and Biologymentioning

confidence: 99%

Comparative Genomics of Gene Expression in the Parasitic and Free-Living Nematodes Strongyloides stercoralis and Caenorhabditis elegans

Mitreva¹,

McCarter²,

Martin³

et al. 2004

Genome Res.

View full text Add to dashboard Cite

Although developmental timing of gene expression is used to infer potential gene function, studies have yet to correlate this information between species. We analyzed 10,921 ESTs in 3311 clusters from first-and infective third-stage larva (L1, L3i) of the parasitic nematode Strongyloides stercoralis and compared the results to Caenorhabditis elegans, a species that has an L3i-like dauer stage. In the comparison of S. stercoralis clusters with stage-specific expression to C. elegans homologs expressed in either dauer or nondauer stages, matches between S. stercoralis L1 and C. elegans nondauer-expressed genes dominated, suggesting conservation in the repertoire of genes expressed during growth in nutrient-rich conditions. For example, S. stercoralis collagen transcripts were abundant in L1 but not L3i, a pattern consistent with C. elegans collagens. Although a greater proportion of S. stercoralis L3i than L1 genes have homologs among the C. elegans dauer-specific transcripts, we did not uncover evidence of a robust conserved L3i/dauer 'expression signature.' Strikingly, in comparisons of S. stercoralis clusters to C. elegans homologs with RNAi knockouts, those with significant L1-specific expression were more than twice as likely as L3i-specific clusters to match genes with phenotypes. We also provide functional classifications of S. stercoralis clusters.

show abstract

The neurons of class ALD mediate thermotaxis in the parasitic nematode, Strongyloides stercoralis

Cited by 52 publications

References 19 publications

Urocanic acid is a major chemoattractant for the skin-penetrating parasitic nematode Strongyloides stercoralis

Urocanic acid is a major chemoattractant for the skin-penetrating parasitic nematode Strongyloides stercoralis

Strongyloides stercoralis: Cell- and tissue-specific transgene expression and co-transformation with vector constructs incorporating a common multifunctional 3′ UTR

Comparative Genomics of Gene Expression in the Parasitic and Free-Living Nematodes Strongyloides stercoralis and Caenorhabditis elegans

Contact Info

Product

Resources

About