The Neurology of Myelin Diseases

Traugott, Ute; Raine, Cedric S.

doi:10.1007/978-1-4757-1830-0_9

Cited by 5 publications

(1 citation statement)

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“…Myelin, segments of lipid-enriched glial cell membrane wrapped around axons, is required for normal saltatory conduction of electrical impulses along axons (for review, see Ritchie, 1984a). Thus, demyelination, characteristic of a number of disease and toxic states (for reviews, see Morel1 et al, 1981;Traugott and Raine, 1984) leads to blocked impulse conduction along axons and therefore impaired neurotransmission (see review by Ritchic, 1984b). However, the interaction between axons and their ensheathing myelin extends beyond facilitation of impulse conduction.…”

Section: Focal Demyelinationmentioning

confidence: 99%

Rapid axonal transport in focally demyelinated sciatic nerve

1987

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Focal demyelination was produced in rat sciatic nerve by unilateral intraneural injection of anti-galactocerebroside serum. A functional lesion was confirmed by the presence of nerve conduction block. Histologically, this corresponded to demyelination of 50-70% of the fibers in nerve cross sections; axonal structures appeared intact. At the time of maximal demyelination (7 d), 35S-methionine or 3H-fucose was injected bilaterally into the spinal cord ventral horn. At later times (5 hr-7 d), the sciatic nerve was removed and radioactivity in successive nerve segments was quantitated. The transport rates (approximately 260 mm/d) and the composition of transported proteins and glycoproteins (separated on 7-15% polyacrylamide gradient gels) were not altered in lesioned nerves relative to contralateral control nerves. Light microscopic autoradiographic analysis revealed a similar localization of axonally transported and deposited glycoproteins in demyelinated and control fibers. Initially (8 hr), the majority of label was over axons. Labeled glycoproteins remaining in the nerve after 1 week were retained mainly in axolemmal regions. We conclude that acute focal primary demyelination does not lead to major alterations in the transport or deposition of newly synthesized macromolecules.

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Section: Focal Demyelinationmentioning

confidence: 99%