The neuroattenuation of three clone viruses isolated from the attenuated CTN rabies virus

Abstract

Three attenuated rabies virus strains(CTN181-3，CTN181-16 and CTN181-19), were selected by plaque cloning from CTN-181 rabies attenuated virus，which was infected guinea pigs submandibular glands.After passaging those viruses in suckling mice brain for five consecutive passages or in submandibular gland of guinea pigs for one passage, non-virulence reversion or increase were detected.The neuroattenuation stability make the three newly selected CTN attenuated strains the promising live vaccine candidates for wild animal prophylactic vaccination and even postexposure prophylaxis of rabies for humans.