The NET Effect of Neutrophils during Helminth Infection

El-Naccache, Darine W.; Chen, Fei; Chen, Neil; Gause, William C.

doi:10.1016/j.chom.2020.01.013

Cited by 8 publications

(8 citation statements)

References 10 publications

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“…The role of neutrophils in type 2 immune responses, particularly in helminth infection, is increasingly appreciated. 68 Here, we focus on the indirect role of NETs in driving type 2 protective immunity to hookworm.…”

Section: Neutrophil‐mediated Killing Of Hookwormsmentioning

confidence: 99%

Hookworm infections: Reappraising the evidence for a role of neutrophils in light of NETosis

Doolan

Bouchery

2022

Parasite Immunology

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In Hookworm infection, neutrophils have long had the image of the villain, being recruited to the site of larval migration because of damage but participating themselves in tissue injury. With recent developments in neutrophil biology, there is an increasing body of evidence for the role of neutrophils as effector cells in hookworm immunity. In particular, their ability to release extracellular traps, or neutrophil extracellular traps (NETs), confer neutrophils a larvicidal activity. Here, we review recent evidence in this nascent field and discuss the avenue for future research on NETs/hookworm interactions.

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Section: Neutrophil‐mediated Killing Of Hookwormsmentioning

confidence: 99%

Hookworm infections: Reappraising the evidence for a role of neutrophils in light of NETosis

Doolan

Bouchery

2022

Parasite Immunology

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“…For example, ROB had higher abundance of neutrophils and platelets, both of which play important roles in parasite defenses. Neutrophils and other granulocyte cells such as eosinophils are important components of the initial innate immune response to helminths and other parasites ( Chen et al 2014 ; El-Naccache et al 2020 ). Platelets, specifically thrombocyte-derived compounds, are important mediators of fibrotic responses ( Antoniades et al 1990 ; Abdollahi et al 2005 ), and fibrosis is a major part of Roberts Lake sticklebacks’ response to S. solidus infection ( Weber et al 2022 ).…”

Section: Resultsmentioning

confidence: 99%

Single-Cell RNA Sequencing Reveals Microevolution of the Stickleback Immune System

Fuess

Bolnick

2023

Genome Biology and Evolution

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The risk and severity of pathogen infections in humans, livestock, or wild organisms depends on host immune function, which can vary between closely related host populations or even among individuals. This immune variation can entail between-population differences in immune gene coding sequences, copy number, or expression. In recent years many studies have focused on population divergence in immunity using whole-tissue transcriptomics. But, whole-tissue transcriptomics cannot distinguish between evolved differences in gene regulation within cells, versus changes in cell composition within the focal tissue. Here we leverage single cell transcriptomic approaches to document signatures of microevolution of immune system structure in a natural system, the three-spined stickleback (Gasterosteus aculeatus). We sampled nine adult fish from three populations with variability in resistance to a cestode parasite, Schistocephalus solidus, to create the first comprehensive immune cell atlas for G. aculeatus. Eight broad immune cell types, corresponding to major vertebrate immune cells, were identified. We were also able to document significant variation in both abundance and expression profiles of the individual immune cell types among the three populations of fish. Furthermore, we demonstrate that identified cell type markers can be used to reinterpret traditional transcriptomic data: we re-evaluate previously published whole-tissue transcriptome data from a quantitative genetic experimental infection study to gain better resolution relating infection outcomes to inferred cell type variation. Combined our study demonstrates the power of single cell sequencing to not only document evolutionary phenomena (i.e. microevolution of immune cells), but also increase the power of traditional transcriptomic datasets.

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“…For example, Roberts lake fish had higher abundance of both neutrophils and platelets, which may contribute to enhanced resistance to helminth parasites. Neutrophils and other granulocyte cells such as eosinophils are important components of the initial innate immune response to helminths and other parasites (49, 50). Platelets, specifically thrombocyte-derived compounds, are important mediators of fibrotic responses (51, 52), and fibrosis is a major part of Roberts Lake sticklebacks’ response to S. solidus infection (48).…”

Section: Resultsmentioning

confidence: 99%

“…Both HCs and RBCs in Roberts had significantly higher expression of neutrophil marker genes ( Figure 3 ). This is likely the result of enhanced overall investment in neutrophil-like cells in Roberts fish, which may support a quick initial response to invading parasites (49, 50). Perhaps most interestingly, we observed population-specific, preferential expression of what is presumably duplicated copies of the important zebrafish neutrophil marker gene, nephrosin ( npsn ).…”

Section: Resultsmentioning

confidence: 99%

Single-cell RNA sequencing reveals micro-evolution of the stickleback immune system

Fuess

Bolnick

2021

Preprint

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Pathogenic infection is an important driver of many ecological processes. Furthermore, variability in immune function is an important driver of differential infection outcomes. New evidence would suggest that immune variation extends to broad cellular structure of immune systems. However, variability at such broad levels is traditionally difficult to detect in non-model systems. Here we leverage single cell transcriptomic approaches to document signatures of microevolution of immune system structure in a natural system, the three-spined stickleback (Gasterosteus aculeatus). We sampled nine adult fish from three populations with variability in resistance to a cestode parasite, Schistocephalus solidus, to create the first comprehensive immune cell atlas for G. aculeatus. Eight major immune cell types, corresponding to major vertebrate immune cells, were identified. We were also able to document significant variation in both abundance and expression profiles of the individual immune cell types, among the three populations of fish. This variability may contribute to observed variability in parasite susceptibility. Finally, we demonstrate that identified cell type markers can be used to reinterpret traditional transcriptomic data. Combined our study demonstrates the power of single cell sequencing to not only document evolutionary phenomena (i.e. microevolution of immune cells), but also increase the power of traditional transcriptomic datasets.

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The NET Effect of Neutrophils during Helminth Infection

Cited by 8 publications

References 10 publications

Hookworm infections: Reappraising the evidence for a role of neutrophils in light of NETosis

Hookworm infections: Reappraising the evidence for a role of neutrophils in light of NETosis

Single-Cell RNA Sequencing Reveals Microevolution of the Stickleback Immune System

Single-cell RNA sequencing reveals micro-evolution of the stickleback immune system

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