The neonicotinoids thiacloprid, imidacloprid, and clothianidin affect the immunocompetence of honey bees (Apis mellifera L.)

Brandt, Annely; Gorenflo, Anna; Siede, Reinhold; Meixner, Marina D.; Büchler, Ralph

doi:10.1016/j.jinsphys.2016.01.001

Cited by 319 publications

(232 citation statements)

References 81 publications

Supporting

Mentioning

214

Contrasting

Unclassified

Order By: Relevance

“…Pesticides have adverse effects on the bee immune system35. Several studies have revealed that neonicotinoid pesticides such as imidacloprid induce pathogen outbreaks in honeybees1036.…”

Section: Resultsmentioning

confidence: 99%

Regulation of genes related to immune signaling and detoxification in Apis mellifera by an inhibitor of histone deacetylation

Yang

et al. 2017

Sci Rep

View full text Add to dashboard Cite

The western honeybee (Apis mellifera) is essential for the global economy due to its important role in ecosystems and agriculture as a pollinator of numerous flowering plants and crops. Pesticide abuse has greatly impacted honeybees and caused tremendous loss of honeybee colonies worldwide. The reasons for colony loss remain unclear, but involvement of pesticides and pathogen-pesticide interactions has been hypothesized. Histone deacetylase inhibitors (HDACis) inhibit the activity of histone acetylase, which causes the hyperacetylation of histone cores and influences gene expression. In this study, sodium butyrate, an HDACi, was used as a dietary supplement for honeybees; after treatment, gene expression profiles were analyzed using quantitative PCR. The results showed that sodium butyrate up-regulated genes involved in anti-pathogen and detoxification pathways. The bioassay results showed that honeybees treated with sodium butyrate were more tolerant to imidacloprid. Additionally, sodium butyrate strengthened the immune response of honeybees to invasions of Nosema ceranae and viral infections. We also performed a bioassay in which honeybees were exposed to pesticides and pathogens. Our results provide additional data regarding the mechanism by which honeybees react to stress and the potential application of HDACis in beekeeping.

show abstract

“…Pesticides have adverse effects on the bee immune system35. Several studies have revealed that neonicotinoid pesticides such as imidacloprid induce pathogen outbreaks in honeybees1036.…”

Section: Resultsmentioning

confidence: 99%

Regulation of genes related to immune signaling and detoxification in Apis mellifera by an inhibitor of histone deacetylation

Yang

et al. 2017

Sci Rep

View full text Add to dashboard Cite

show abstract

“…These findings and the highly-conserved nature of innate immunity suggest that translation of findings using this D . melanogaster model are relevant to honey bees and will be amenable for future investigation of pesticide interventions in honey bees 18, 39 .…”

Section: Discussionmentioning

confidence: 99%

Neonicotinoid-induced pathogen susceptibility is mitigated by Lactobacillus plantarum immune stimulation in a Drosophila melanogaster model

Daisley

Trinder

McDowell

et al. 2017

Sci Rep

View full text Add to dashboard Cite

Pesticides are used extensively in food production to maximize crop yields. However, neonicotinoid insecticides exert unintentional toxicity to honey bees (Apis mellifera) that may partially be associated with massive population declines referred to as colony collapse disorder. We hypothesized that imidacloprid (common neonicotinoid; IMI) exposure would make Drosophila melanogaster (an insect model for the honey bee) more susceptible to bacterial pathogens, heat stress, and intestinal dysbiosis. Our results suggested that the immune deficiency (Imd) pathway is necessary for D. melanogaster survival in response to IMI toxicity. IMI exposure induced alterations in the host-microbiota as noted by increased indigenous Acetobacter and Lactobacillus spp. Furthermore, sub-lethal exposure to IMI resulted in decreased D. melanogaster survival when simultaneously exposed to bacterial infection and heat stress (37 °C). This coincided with exacerbated increases in TotA and Dpt (Imd downstream pro-survival and antimicrobial genes, respectively) expression compared to controls. Supplementation of IMI-exposed D. melanogaster with Lactobacillus plantarum ATCC 14917 mitigated survival deficits following Serratia marcescens (bacterial pathogen) septic infection. These findings support the insidious toxicity of neonicotinoid pesticides and potential for probiotic lactobacilli to reduce IMI-induced susceptibility to infection.

show abstract

“…Efforts to understand how environmental insults, such as xenobiotic exposure, nutritional status, and parasitism, impact hemocyte-derived immunity represent an emerging area of honey bee research (Szymaś and Jędruszuk 2003;Amdam et al 2004;Brandt et al 2016;Marringa et al 2014). However, such studies are dependent upon a clear understanding of the composition and function of hemocyte communities.…”

Section: Discussionmentioning

confidence: 99%

Morphological and functional characterization of honey bee, Apis mellifera, hemocyte cell communities

et al. 2018

View full text Add to dashboard Cite

-Among invertebrates, cellular innate immunity is critical for wound healing and defense against parasites and pathogens. While the study of cellular immunity has received much attention in model insects, the study of hemocytes, including immune cells, in honey bees has received little attention. Much of our understanding of honey bee hemocytes is derived from a limited set of methodologies, predominately utilizing bright-field microscopy, which makes broad conclusions about honey bee cellular immunity difficult to infer. We build upon existing methodologies using differential cell staining, in vitro phagocytosis assays, and an analysis of respiratory burst activity as measured through reactive oxygen species (ROS) production. Further, we characterize the morphological diversity and functional capacity of honey bee hemocytes in both adult workers and young queen bees as well as the ontogeny of the hemocyte population across larval and adult stages of the worker caste. Our findings suggest that granulocytes are the major phagocytic cells in honey bees and that circulating larval granulocytes undergo mitotic cell division. Additionally, we demonstrate that ROS production in larval hemocytes can be stimulated with the protein kinase C activator, phorbol 12-myristate 13-acetate. This indicates the presence of a functional protein kinase C-dependent phagocyte oxidase system, though further experimentation is needed to confirm phagocyte oxidase as the source of ROS. Overall, this work expands our knowledge of honey bee hemocytes and provides additional methodological tools for studying immune mechanisms in insects. cellular immunity / plasmatocytes / granulocytes / phagocytosis / ROS production

show abstract

The neonicotinoids thiacloprid, imidacloprid, and clothianidin affect the immunocompetence of honey bees (Apis mellifera L.)

Cited by 319 publications

References 81 publications

Regulation of genes related to immune signaling and detoxification in Apis mellifera by an inhibitor of histone deacetylation

Regulation of genes related to immune signaling and detoxification in Apis mellifera by an inhibitor of histone deacetylation

Neonicotinoid-induced pathogen susceptibility is mitigated by Lactobacillus plantarum immune stimulation in a Drosophila melanogaster model

Morphological and functional characterization of honey bee, Apis mellifera, hemocyte cell communities

Contact Info

Product

Resources

About