The Neonatal Ventromedial Hypothalamus Transcriptome Reveals Novel Markers with Spatially Distinct Patterning

Kurrasch, Deborah M.; Cheung, Clement C.; Lee, Florence Y.; Tran, Phu V.; Hata, Kenji; Ingraham, Holly A.

doi:10.1523/jneurosci.2858-07.2007

Cited by 154 publications

(157 citation statements)

References 61 publications

Supporting

Mentioning

151

Contrasting

Order By: Relevance

“…We investigated the metabolic role of PACAP, a pleiotropic neuropeptide, which was recently identified by microarray as a "VMN-enriched" gene (Segal et al, 2005;Kurrasch et al, 2007).…”

Section: Identifying Pacap As a Vmn Phenotypic Markermentioning

confidence: 99%

“…Like many hypothalamic neurons, at least some of the effects of SF-1 cells in the VMN are mediated by glutamate (Tong et al, 2007), although neuropeptidergic classifications remain elusive. Microarray studies have identified a number of VMN-enriched genes, including the neuropeptide pituitary adenylate cyclase-activating polypeptide (PACAP) (Segal et al, 2005;Kurrasch et al, 2007), which has been implicated in many central functions (for review, see Vaudry et al, 2000). The pleiotropic nature of this peptide has made it difficult to study in terms of energy balance; however, in this study, we have shown by in vivo pharmacological and histological methods that the VMN PACAP population partially mediates responses to leptin, and that PACAP exerts robust effects on both appetite and energy expenditure in mice.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

PACAP Neurons in the Hypothalamic Ventromedial Nucleus Are Targets of Central Leptin Signaling

Hawke

Ivanov²,

Bechtold³

et al. 2009

J. Neurosci.

140

View full text Add to dashboard Cite

show abstract

“…We investigated the metabolic role of PACAP, a pleiotropic neuropeptide, which was recently identified by microarray as a "VMN-enriched" gene (Segal et al, 2005;Kurrasch et al, 2007).…”

Section: Identifying Pacap As a Vmn Phenotypic Markermentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

PACAP Neurons in the Hypothalamic Ventromedial Nucleus Are Targets of Central Leptin Signaling

Hawke

Ivanov²,

Bechtold³

et al. 2009

J. Neurosci.

140

View full text Add to dashboard Cite

show abstract

“…2C), novel molecular markers of VMH in the newborn mouse were identified (Kurrasch et al, 2007). Many of these neuroendocrine markers exhibit distinct regional expression patterns in the newly formed hypothalamus.…”

Section: Patterning Of Developing Thalamus and Hypothalamus By Secretmentioning

confidence: 99%

Molecular Pathways Controlling Development of Thalamus and Hypothalamus: From Neural Specification to Circuit Formation

et al. 2010

View full text Add to dashboard Cite

The embryonic diencephalon gives rise to the vertebrate thalamus and hypothalamus, which play essential roles in sensory information processing and control of physiological homeostasis and behavior, respectively. In this review, we present new steps toward characterizing the molecular pathways that control development of these structures, based on findings in a variety of model organisms. We highlight advances in understanding how early regional patterning is orchestrated through the action of secreted signaling molecules such as Sonic hedgehog and fibroblast growth factors. We address the role of individual transcription factors in control of the regional identity and neural differentiation within the developing diencephalon, emphasizing the contribution of recent large-scale gene expression studies in providing an extensive catalog of candidate regulators of hypothalamic neural cell fate specification. Finally, we evaluate the molecular mechanisms involved in the experience-dependent development of both thalamo-cortical and hypothalamic neural circuitry. IntroductionThe developing vertebrate forebrain consists of two major parts: the telencephalon-which gives rise to cerebral cortex, striatum, amygdala, and associated structures-and the diencephalon. The diencephalon gives rise to two essential brain regions, the thalamus and hypothalamus (Fig. 1 A). Though both structures are derived from a common region of the anterior neural tube, they serve very different physiological functions. The thalamus acts as a central integrator of sensory information, receiving afferents from receptors of all sensory modalities save olfaction, and serves as the sole path by which integrated sensory information reaches the cerebral cortex and gives rise to conscious perception. The hypothalamus, on the other hand, serves a more diverse range of functions. It is a central regulator of critical homeostatic physiological processes, including temperature regulation, food intake,

show abstract

“…Because nPE1 and nPE2 are functional analogs, it is conceivable that these sequences recruit a common, yet unidentified TF able to transactivate Pomc in the hypothalamus. Pomc expression starts in the ventral lining of the developing mouse hypothalamus at embryonic day 10.5 (E10.5) (15)(16)(17), a precise spatiotemporal domain in which multiple HDTFs determine the early patterning of the mouse ventral hypothalamus (19,20). Therefore, we considered of interest to investigate whether the terminal differentiation of melanocortin-expressing neurons is regulated by a HDTF.…”

mentioning

confidence: 99%

Islet 1 specifies the identity of hypothalamic melanocortin neurons and is critical for normal food intake and adiposity in adulthood

Nasif

Souza

González

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Food intake and body weight regulation depend on proper expression of the proopiomelanocortin gene (Pomc) in a group of neurons located in the mediobasal hypothalamus of all vertebrates. These neurons release POMC-encoded melanocortins, which are potent anorexigenic neuropeptides, and their absence from mice or humans leads to hyperphagia and severe obesity. Although the pathophysiology of hypothalamic POMC neurons is well understood, the genetic program that establishes the neuronal melanocortinergic phenotype and maintains a fully functional neuronal POMC phenotype throughout adulthood remains unknown. Here, we report that the early expression of the LIM-homeodomain transcription factor Islet 1 (ISL1) in the developing hypothalamus promotes the terminal differentiation of melanocortinergic neurons and is essential for hypothalamic Pomc expression since its initial onset and throughout the entire lifetime. We detected ISL1 in the prospective hypothalamus just before the onset of Pomc expression and, from then on, Pomc and Isl1 coexpress. ISL1 binds in vitro and in vivo to critical homeodomain binding DNA motifs present in the neuronal Pomc enhancers nPE1 and nPE2, and mutations of these sites completely disrupt the ability of these enhancers to drive reporter gene expression to hypothalamic POMC neurons in transgenic mice and zebrafish. ISL1 is necessary for hypothalamic Pomc expression during mouse and zebrafish embryogenesis. Furthermore, conditional Isl1 inactivation from POMC neurons impairs Pomc expression, leading to hyperphagia and obesity. Our results demonstrate that ISL1 specifies the identity of hypothalamic melanocortin neurons and is required for melanocortin-induced satiety and normal adiposity throughout the entire lifespan.hypothalamus | melanocortin | obesity | Isl1 | pomc

show abstract

The Neonatal Ventromedial Hypothalamus Transcriptome Reveals Novel Markers with Spatially Distinct Patterning

Cited by 154 publications

References 61 publications

PACAP Neurons in the Hypothalamic Ventromedial Nucleus Are Targets of Central Leptin Signaling

PACAP Neurons in the Hypothalamic Ventromedial Nucleus Are Targets of Central Leptin Signaling

Molecular Pathways Controlling Development of Thalamus and Hypothalamus: From Neural Specification to Circuit Formation

Islet 1 specifies the identity of hypothalamic melanocortin neurons and is critical for normal food intake and adiposity in adulthood

Contact Info

Product

Resources

About