The nematode Caenorhabditis elegans as an integrated toxicological tool to assess water quality and pollution

Clavijo, Araceli; Kronberg, Florencia; Rossen, Ariana; Moya, Aldana; Calvo, Daniel; Salatino, S.; Pagano, Eduardo; Morábito, José; Munarriz, Eliana

doi:10.1016/j.scitotenv.2016.06.057

Cited by 29 publications

(18 citation statements)

References 39 publications

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“…At the genetic level, more than half of the conserved signaling regions in humans are found in the nematode (Aoki and Mori, 2015). Numerous studies have reported the sensitivity of C. elegans in terms of pollution from different types of environmental media (Clavijo et al, 2016;Zuo et al, 2017;Rai et al, 2019). However, only a few studies have reported the sensitivity of C. elegans to air pollutants, specifically, to PM 2.5 (Zhao et al, 2014;Sun et al, 2015Sun et al, , 2016.…”

Section: Introductionmentioning

confidence: 99%

Fine Particulate Matter-induced Toxic Effects in an Animal Model of Caenorhabditis elegans

Chung

Tsai

Que

et al. 2019

Aerosol Air Qual. Res.

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Research has been focused on the health hazards of ambient PM 2.5 related to humans. Many PM 2.5 toxicity assessments using in vitro studies have focused on PM 2.5 -bounded hazardous pollutants. However, PM 2.5 toxicity assessment by in vivo studies allow for better observation of the overall effects of PM 2.5 exposure on entire organisms, making in vivo PM 2.5 toxicity assessment relevant. The toxic effects of outdoor PM 2.5 , collected from

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Section: Introductionmentioning

confidence: 99%

Fine Particulate Matter-induced Toxic Effects in an Animal Model of Caenorhabditis elegans

Chung

Tsai

Que

et al. 2019

Aerosol Air Qual. Res.

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“…In this study, for the first time, we investigate the toxicological effects of topoisomerase inhibitors, both in the soil nematode Caenorhabditis elegans and 3T3‐L1 normal murine cells. C. elegans is a soil nematode that has been used for the environmental toxicity assessment of various chemicals . We chose etoposide as a model topoisomerase inhibitor because etoposide is used as a clinical anticancer agent to treat cancer patients.…”

Section: Introductionmentioning

confidence: 99%

“…C. elegans is a soil nematode that has been used for the environmental toxicity assessment of various chemicals. 12 We chose etoposide as a model topoisomerase inhibitor because etoposide is used as a clinical anticancer agent to treat cancer patients. We not only measured the inhibitory effects of etoposide on the growth of C. elegans and 3T3-L1 cells, but we also observed the morphological changes of nuclei in both somatic and reproductive cells.…”

Section: Introductionmentioning

confidence: 99%

Toxicological evaluation of the topoisomerase inhibitor, etoposide, in the model animal Caenorhabditis elegans and 3T3‐L1 normal murine cells

Lee

Kim

Jung

et al. 2017

Environmental Toxicology

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Etoposide, a topoisomerase II inhibitor, has been widely used as a clinical anticancer drug to treat diverse cancer patients. Since not only rapidly dividing cancer cells but also the cells of normal human tissues and every living organism in environmental ecosystems have topoisomerases, it is crucial to study the toxicity of etoposide in other organisms in addition to cancer cells. In this study, we evaluated the toxicity of etoposide in both a soil nematode, Caenorhabditis elegans, and 3T3-L1 normal murine cells. Etoposide significantly retarded the growth, egg laying, and hatching in C. elegans. Etoposide also affected the reproductive gonad tissue, decreased the number of germ cells and induced abnormally enlarged nuclei in C. elegans. In addition, etoposide inhibited 3T3-L1 cell proliferation, with IC values of 37.8 ± 7.3 and 9.8 ± 1.8 μM after 24 and 48 hours of treatment, respectively, via the induction of cell cycle arrest at the G2/M phase and apoptotic cell death. Etoposide also induced nuclear enlargement in 3T3-L1 normal murine cells. The reproductive toxicity and abnormal nuclear morphological changes seemed to correlate with the adverse effects of etoposide. We suggest that these experimental platforms, i.e., the toxicological evaluation of both nematodes and 3T3-L1 cells, may be useful to study the mechanisms underlying the side effects of chemicals, including topoisomerase inhibitors.

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“…O acelerado crescimento populacional e a expansão agrícola e industrial nas últimas décadas demandaram maior utilização dos recursos hídricos, o que também causou a degradação dos ambientes aquáticos (Bertoldo et al 2016). Ações antrópicas, como: (a) o lançamento de efluentes; (b) a disposição de resíduos sólidos e (c) a infiltração de lixiviados, contribuem para a contaminação dos corpos de água com agentes patogênicos e resíduos químicos, o que compromete a qualidade das águas superficiais e subterrâneas, afetando a saúde e a segurança da população (Clavijo et al 2016;Lenart-Boroń et al 2017).…”

Section: Introductionunclassified

Avaliação toxicológica de ambientes aquáticos: importância da análise em sedimento

Silva

Machado

Zagui

et al. 2020

G. Sci.

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A contaminação dos recursos hídricos pode afetar a saúde humana e ambiental. Em ambientes aquáticos, os sedimentos são relevantes para a sobrevivência da biota. No entanto, a maioria dos contaminantes se encontra armazenada nessa matriz, representando riscos para os organismos. A toxicidade aguda de sedimento e água superficial foi avaliada utilizando a bactéria Vibrio fischeri, por meio do sistema Microtox®. As amostras foram coletadas ao longo do Rio Pardo, São Paulo, Brasil, durante as estações chuvosa e seca. Nos sedimentos, foram detectadas significativas porcentagens de CE20, com predominância de concentração Tóxica e Muito Tóxica. Não foi verificada toxicidade aguda à bactéria nas análises realizadas com as amostras de água. Em conclusão, a presença de compostos tóxicos no sedimento do Rio Pardo indica que há impacto ambiental iminente e o acúmulo desses contaminantes nessa matriz é mais significativo quando comparado à água. Portanto, as amostras de sedimento analisadas mostraram que essa matriz retrata de maneira mais confiável a qualidade do ecossistema.

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The nematode Caenorhabditis elegans as an integrated toxicological tool to assess water quality and pollution

Cited by 29 publications

References 39 publications

Fine Particulate Matter-induced Toxic Effects in an Animal Model of Caenorhabditis elegans

Fine Particulate Matter-induced Toxic Effects in an Animal Model of Caenorhabditis elegans

Toxicological evaluation of the topoisomerase inhibitor, etoposide, in the model animal Caenorhabditis elegans and 3T3‐L1 normal murine cells

Avaliação toxicológica de ambientes aquáticos: importância da análise em sedimento

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