The nematocysts venom of Chrysaora helvola Brandt leads to apoptosis-like cell death accompanied by uncoupling of oxidative phosphorylation

Qu, Xiaosheng; Fan, Lan-Lan; Zhong, Taozheng; Li, Gang; Xia, Xianghua; Long, Hairong; Huang, Danna; Wei, Shu

doi:10.1016/j.toxicon.2015.12.011

Cited by 8 publications

(5 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Controlling cell migration during normal repair or development, excessive production can also aggravate the progression of inflammation. These three inflammatory factors are used as indicators of inflammation to explore the inflammatory effects of other toxins, for example, IL-6 and TNF-α were used as indicators of inflammation to explore the inflammatory effects of snake venom [23]. MCP-1 and IL-6 were used to explore the inflammatory effects of spider venom [24].…”

Section: Discussionmentioning

confidence: 99%

Inhibitory Effect of Metalloproteinase Inhibitors on Skin Cell Inflammation Induced by Jellyfish Nemopilema nomurai Nematocyst Venom

et al. 2019

Toxins

View full text Add to dashboard Cite

Jellyfish envenomations result in extensive dermatological symptoms, clinically named as jellyfish dermatitis, which can seriously affect the daily activities and physical health of people. Inflammatory response accompanies the whole process of jellyfish dermatitis and the complexity of jellyfish venom components makes it difficult to treat jellyfish dermatitis symptoms effectively. Moreover, inhibiting inflammation is essential for the treatment of jellyfish stings and exploring the main components of jellyfish venom that cause inflammation is an urgent research area. In this study, the inhibitory effects of matrix metalloproteinase (MMP) inhibitors for venom-induced inflammation were explored at a cellular level. The expression of the three inflammatory factors, IL-6, TNF-α and MCP-1 in two skin cell lines, human keratinocyte cells (HaCaT) and human embryonic skin fibroblasts cells (CCC-ESF-1), at the cellular level, after treatment with the inhibitors of jellyfish Nemopilema nomurai (N. nomurai) nematocyst venom (NnNV-I), were determined. The results showed that inhibitors of MMP can significantly reduce the toxic effects of jellyfish Nemopilema nomurai nematocyst venom (NnNV) to skin cells. The expression levels of the three inflammatory factors IL-6, MCP-1, and TNF-α in the cells were also significantly decreased, indicating that MMPs in jellyfish venom are probably vital factors leading to jellyfish dermatitis. This study is beneficial in the prevention and treatment of jellyfish stings.

show abstract

Section: Discussionmentioning

confidence: 99%

Inhibitory Effect of Metalloproteinase Inhibitors on Skin Cell Inflammation Induced by Jellyfish Nemopilema nomurai Nematocyst Venom

et al. 2019

Toxins

View full text Add to dashboard Cite

show abstract

“…Human cancer cell lines are often used to elucidate the harmful effects of venoms on humans and to understand the molecular mechanisms of venom toxicity, and they have been used on numerous occasions for jellyfish venom toxins [ 57 , 65 , 72 , 118 , 123 , 124 , 129 ]. They provide a reliable, high-throughput, convenient, and relatively cheap approach [ 124 ], which is primarily relevant for medical and pharmaceutical applications, specifically when it comes to first aid treatments regarding understudied jellyfish venom stings [ 123 , 124 ]. This model, however, has its own limitation, with a bias for the most accessible cell lines available, thus causing variation in the obtained results [ 57 , 65 , 72 , 118 , 123 , 124 , 129 ].…”

Section: Discussionmentioning

confidence: 99%

“…They provide a reliable, high-throughput, convenient, and relatively cheap approach [ 124 ], which is primarily relevant for medical and pharmaceutical applications, specifically when it comes to first aid treatments regarding understudied jellyfish venom stings [ 123 , 124 ]. This model, however, has its own limitation, with a bias for the most accessible cell lines available, thus causing variation in the obtained results [ 57 , 65 , 72 , 118 , 123 , 124 , 129 ]. The most common mechanistic function reported using these cell lines involved reactive oxygen species (ROS) and cell apoptosis.…”

Section: Discussionmentioning

confidence: 99%

Investigation of Best Practices for Venom Toxin Purification in Jellyfish towards Functional Characterisation

Lausen

Ahang

Cummins

et al. 2023

Toxins

View full text Add to dashboard Cite

The relative lack of marine venom pharmaceuticals can be anecdotally attributed to difficulties in working with venomous marine animals, including how to maintain venom bioactivity during extraction and purification. The primary aim of this systematic literature review was to examine the key factors for consideration when extracting and purifying jellyfish venom toxins to maximise their effectiveness in bioassays towards the characterisation of a single toxin.An up-to-date database of 119 peer-reviewed research articles was established for all purified and semi-purified venoms across all jellyfish, including their level of purification, LD50, and the types of experimental toxicity bioassay used (e.g., whole animal and cell lines). We report that, of the toxins successfully purified across all jellyfish, the class Cubozoa (i.e., Chironex fleckeri and Carybdea rastoni) was most highly represented, followed by Scyphozoa and Hydrozoa. We outline the best practices for maintaining jellyfish venom bioactivity, including strict thermal management, using the “autolysis” extraction method and two-step liquid chromatography purification involving size exclusion chromatography. To date, the box jellyfish C. fleckeri has been the most effective jellyfish venom model with the most referenced extraction methods and the most isolated toxins, including CfTX-A/B. In summary, this review can be used as a resource for the efficient extraction, purification, and identification of jellyfish venom toxins.

show abstract

“…The percentage of cells that were Annexin V-positive represented the proportion of apoptotic cells. Alternatively, apoptosis was also measured by TUNEL staining using a One Step TUNEL Apoptosis Assay Kit (Beyotime) [16]. Nuclear staining was evaluated under a light microscope via DAPI staining (Beyotime).…”

Section: Methodsmentioning

confidence: 99%

Immunotherapy of Dual-Function Vector with Both Immunostimulatory and B-Cell Lymphoma 2 (Bcl-2)-Silencing Effects on Gastric Carcinoma

Han

Keyoumu

et al. 2017

Med Sci Monit

View full text Add to dashboard Cite

BackgroundTumorigenesis is a kind of pathology marked by infinite proliferation and restrained apoptosis compared with normal cells. The abnormal expression of some proto-oncogenes and apoptosis inhibition are essential for tumor growth, which has been confirmed by molecular biologic and immunologic studies. The hypofunction of the host immune system also drives the development and metastasis of malignant tumors. Bcl-2, which has a critical role in regulating apoptosis, is overexpressed in several cancers.Material/MethodsIn this study, we constructed a dual-function small hairpin RNA (shRNA) vector containing an Bcl-2-silencing shRNA and a TLR7-stimulating ssRNA and examined it effect on tumor cell growth and proliferation.ResultsStimulation with this bi-functional vector in vitro promoted significant apoptosis of MFC cells by regulating the expression of apoptosis-related proteins and induced secretion of type I IFNs. Most importantly, this bi-functional vector more effectively inhibited subcutaneous MFC cell growth than did single shRNA and ssRNA treatment in vivo. Natural killer (NK) and CD4+ T cells were required for effective tumor suppression, and TLR7 was shown to play a helper role in the activation of NK cells and CD4+ T cells, possibly by regulating the expression of receptors or secretion of cytokines.ConclusionsThis bi-functional vector that contained ssRNA and shRNA may represent a promising approach for tumor therapy.

show abstract

The nematocysts venom of Chrysaora helvola Brandt leads to apoptosis-like cell death accompanied by uncoupling of oxidative phosphorylation

Cited by 8 publications

References 31 publications

Inhibitory Effect of Metalloproteinase Inhibitors on Skin Cell Inflammation Induced by Jellyfish Nemopilema nomurai Nematocyst Venom

Inhibitory Effect of Metalloproteinase Inhibitors on Skin Cell Inflammation Induced by Jellyfish Nemopilema nomurai Nematocyst Venom

Investigation of Best Practices for Venom Toxin Purification in Jellyfish towards Functional Characterisation

Immunotherapy of Dual-Function Vector with Both Immunostimulatory and B-Cell Lymphoma 2 (Bcl-2)-Silencing Effects on Gastric Carcinoma

Contact Info

Product

Resources

About