The neglected hypogeous fungus Hydnotrya bailii Soehner (1959) is a widespread sister taxon of Hydnotrya tulasnei (Berk.) Berk. & Broome (1846)

Abstract

The neglected false truffle species Hydnotrya bailii Soehner (Ascomycetes, Discinaceae) is re-described and separated from its sister taxon Hydnotrya tulasnei by morphological and phylogenetic analyses based on internal transcribed spacer rDNA sequences. The most distinct morphological and ecological characters are small globose, rather than kidney-like, ascomata as known from the sister taxon H. tulasnei, strictly monoseriate ascospores and montane habitats. Phylogenetic analyses resulted in two clearly separated clusters that revealed the ectomycorrhizal specificity of H. bailii to Picea abies and that H. tulasnei is preferably associated to Fagus sylvatica. We also show that H. bailii was already present in mycorrhizal samples but until now could not be correctly assigned. Our analyses also indicate cryptic diversity within H. cerebriformis and other, morphologically not yet characterized, Hydnotrya groups.An emended determination key for all Hydnotrya species known from Central Europe is provided.

Tzeananiaceae, a new pleosporalean family associated with Ophiocordyceps macroacicularis fruiting bodies in Taiwan

“…The PCR reactions for amplification of the internal transcribed spacer regions 1 and 2 flanking the 5.8S nrRNA gene (ITS) (Schoch et al 2012), were performed under standard conditions (White et al 1990, Stielow et al 2010). PCR conditions for amplification of the partial SSU (Small subunit of the nrRNA gene) and LSU (Large subunit of the nrRNA gene) followed the protocol of Ariyawansa et al (2015).…”

Novel genera and species of coniothyrium-like fungi in Montagnulaceae (Ascomycota)

“…The PCR reactions for amplification of the recently ratified universal fungal barcode ITS1-5.8S-ITS2 of the nuclear ribosomal DNA operon (Schoch et al 2012), using ITS5/ITS1 and ITS4 were performed under standard or semi-nested conditions (White et al 1990, Stielow et al 2010). PCR conditions for amplifying the partial LSU rDNA using the standard primers LR0R and LR5 only differed in their annealing temperature (55 °C instead of 60 °C) and increased cycle extension time (90 s per cycle).…”

A comprehensive molecular phylogeny of the <I>Mortierellales</I> (<I>Mortierellomycotina</I>) based on nuclear ribosomal DNA

“…The PCR for the amplification of the ITS1-5.8S-ITS2 nuclear ribosomal DNA region uses ITS5/ITS1 and ITS4 under standard or semi-nested conditions (White et al 1990, Stielow et al 2009). PCR for amplifying the partial 28S rDNA (LSU) was done using the standard primers LR0R and LR5 or the NL-primer (http://www.biology.duke.edu/fungi/mycolab/primers.htm).…”