The needle mycobiome of Picea glauca – A dynamic system reflecting surrounding environment and tree phenological traits

Würth, David G.; Dahl, Mathilde Borg; Trouillier, Mario; Wilmking, Martin; Unterseher, Martin; Schöller, M.; Sørensen, Søren J.; Mortensen, Martin Steen; Schnittler, Martin

doi:10.1016/j.funeco.2019.05.006

Cited by 18 publications

(20 citation statements)

References 73 publications

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“…Other influencing factors such as light or moisture will also vary across sites, affecting both the host phenotype and the capacity of microbes to infect the tree. Our study adds new evidence to the fact that the tree microbiome can be dynamic (Würth et al, 2019) that is, the microbes will react and change their relative abundance as a result of host stress, therefore adding a new dimension to this complex system.…”

Section: Sydowia Polysporamentioning

confidence: 90%

Competitive exclusion amongst endophytes determines shoot blight severity on pine

et al. 2020

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1. The role of competitive interactions among endophytes and latent pathogens that determine disease on stressed plants is largely unknown. 2. Shoot blight caused by Diplodia sapinea is an emerging disease affecting pine forests in Europe. D. sapinea is an endophyte that becomes pathogenic following drought or hail. Disease expression depends on the availability of nutrient-rich metabolites produced by trees to alleviate stress. We studied whether competition for these compounds with other endophytes could modulate symptom expression. 3. We compared the shoot-inhabiting fungal community of trees that developed blight symptoms or remained asymptomatic following a hailstorm that affected four pine species. Communities were characterised by isolation and metabarcoding of the ITS2 region. Endophytes were functionally annotated in their response to C, N and proline. 4. Hail promoted D. sapinea and other endophytes with a rapid colonisation strategy of N-rich substrates. Asymptomatic trees had a community of endophytes showing a strong negative association with D. sapinea. These antagonistic endophytes were functionally similar to D. sapinea in their response traits to hail, but they were more specialised to proline. The association was conserved across pine species. 5. Our data suggest that after a stress-inducing event, rapid niche occupation is critical for D. sapinea to cause disease. However, competition with other endophytes for key metabolites can supress the pathogen and prevent trees from developing symptoms. 6. Our findings can pave the way to a better understanding of the contribution of latent pathogens on widespread tree mortality events involving abiotic stressors, such as drought, heat or hail.

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Section: Sydowia Polysporamentioning

confidence: 90%

Competitive exclusion amongst endophytes determines shoot blight severity on pine

et al. 2020

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“…Internal factors may include host plant species and genotype (Unterseher et al, 2007(Unterseher et al, , 2012Peršoh et al, 2010;Cordier et al, 2012;Bálint et al, 2013;Peršoh, 2013;Weig et al, 2013;Albrectsen et al, 2018); plant organ, e.g., leaves, twigs, and stems (Sieber and Hugentobler, 1987;Petrini, 1991;Sahashi et al, 1999;Santamaría and Diez, 2005); plant condition (Agostinelli et al, 2018); and plant development (Jumpponen and Jones, 2010;Vořıšková and Baldrian, 2013;Baldrian, 2017). External factors may include structure and diversity of the surrounding vegetation (Helander et al, 2007), antagonists such as herbivores (Albrectsen et al, 2018), local climate conditions (Hashizume et al, 2008;Cordier et al, 2012;Eusemann et al, 2016;Würth et al, 2019), geographical location (U' Ren et al, 2012), and seasonality (Karlsson et al, 2020). The complexity of the mycobiome is thus well established, although ecological insights have mostly been founded on cultivationbased techniques that tend to underestimate diversity (Siddique et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Mycobiomes of Young Beech Trees Are Distinguished by Organ Rather Than by Habitat, and Community Analyses Suggest Competitive Interactions Among Twig Fungi

Siddique

Biella

Unterseher³

et al. 2021

Front. Microbiol.

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Beech trees (Fagus sylvatica) are prominent keystone species of great economic and environmental value for central Europe, hosting a diverse mycobiome. The composition of endophyte communities may depend on tree health, plant organ or tissue, and growth habitat. To evaluate mycobiome communalities at local scales, buds, and twigs were sampled from two young healthy mountain beech stands in Bavaria, Germany, four kilometers apart. With Illumina high-throughput sequencing, we found 113 fungal taxa from 0.7 million high-quality reads that mainly consisted of Ascomycota (52%) and Basidiomycota (26%) taxa. Significant correlations between richness and diversity indices were observed (p < 0.05), and mycobiomes did not differ between habitats in the current study. Species richness and diversity were higher in twigs compared to spring buds, and the assemblages in twigs shared most similarities. Interaction network analyses revealed that twig-bound fungi shared similar numbers of (interaction) links with others, dominated by negative co-occurrences, suggesting that competitive exclusion may be the predominant ecological interaction in the highly connected twig mycobiome. Combining community and network analyses strengthened the evidence that plant organs may filter endophytic communities directly through colonization access and indirectly by facilitating competitive interactions between the fungi.

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“…The colour scale illustrates the dominance (%) within the sites. The dendrogram on top illustrates the similarity of the sites; the one on the left represents the similarity in the pattern of occurrence of the individual fungal OTUs and Corlett 2019; Egidi et al 2019;Epstein et al 2019;Li et al 2019;Lynikiene et al 2020;Morales-Rodriguez et al 2019;Nerva et al 2019;Qian et al 2020;Ricks and Koide 2019;Saravesi et al 2019;Wilkinson et al 2019;Würth et al 2019;Yao et al 2019;Zhang et al 2019), others strongly recommended the use of ITS1 (Mbareche et al 2020), as it had already been implemented in many studies (Beule et al 2019;Checinska Sielaff et al 2019;Del Frari et al 2019;Schiro et al 2019). Few studies are based on both ITS regions (Janakiev et al 2019).…”

Section: Applied Methodsmentioning

confidence: 99%

“…The use of operational taxonomic units (OTUs) versus the recent use of amplicon sequence variants (ASVs) is also inconsistent. Although there are recommendations to use only ASVs (Callahan et al 2017), resulting in a reduction in the number of dominant species (Egidi et al 2019), many of the current studies are still based on OTUs (Bao et al 2019;Beng and Corlett 2019;Beule et al 2019;Checinska Sielaff et al 2019;Epstein et al 2019;Janakiev et al 2019;Lynikiene et al 2020;Morales-Rodriguez et al 2019;Nerva et al 2019;Pan et al 2019;Qian et al 2020;Ricks and Koide 2019;Saravesi et al 2019;Wilkinson et al 2019;Würth et al 2019;Yao et al 2019;Zhang et al 2019), but others rely on ASVs (Del Frari et al 2019;Egidi et al 2019;Li et al 2019), or OTUs generated from ASVs (Ezeokoli et al 2020), and even OTUs from cultivationbased Sanger sequencing are still in use (Janowsky et al 2019). With the availability of the VSEARCH tool within the QIIME2 pipeline, allowing the easy aggregation of OTUs from ASVs, the Illumina sequencing datasets in this Fig.…”

Section: Applied Methodsmentioning

confidence: 99%

“…Most current studies do not distinguish between epiphytic and endophytic fungal communities, as the complete microbiome is usually extracted from whole leaves (Lynikiene et al 2020;Qian et al 2020;Würth et al 2019). This is problematic in that endophytic fungi are more hostdependent than epiphytic fungi (Fonseca-García et al 2016;Lau et al 2013;Peršoh 2013).…”

Section: Applied Methodsmentioning

confidence: 99%

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Comparative analyses of sooty mould communities from Brazil and Central Europe

et al. 2021

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To gain an insight into fungal sooty mould communities on leaves of trees and shrubs in the tropics and in temperate regions, 47 biofilms of the Mata Atlântica rainforest relic and the Caatinga vegetation in the state of Sergipe, Northeast Brazil, and from Central European colline and alpine zones were compared. The four sampling sites clearly differed in composition of their epiphyllous fungal communities. The fungal OTUs from all sites belonged mainly to the Ascomycota, with Dothideomycetes being the dominant class. The core community group consisted of a few site-specific representatives in co-occurrence with the ubiquitous Mycosphaerella tassiana and Aureobasidium pullulans. Most species of the core community were dark pigmented and were accompanied by facultative unpigmented or lightly pigmented species. Among the cultivable fungal species, the proportion of melanised species was significantly more abundant in samples from the two European sites, which supports the theory of thermal melanism. The identity of the host plant had a stronger impact on fungal community composition than the presence of sap-feeding insects.

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The needle mycobiome of Picea glauca – A dynamic system reflecting surrounding environment and tree phenological traits

Cited by 18 publications

References 73 publications

Competitive exclusion amongst endophytes determines shoot blight severity on pine

Competitive exclusion amongst endophytes determines shoot blight severity on pine

Mycobiomes of Young Beech Trees Are Distinguished by Organ Rather Than by Habitat, and Community Analyses Suggest Competitive Interactions Among Twig Fungi

Comparative analyses of sooty mould communities from Brazil and Central Europe

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