The NCI In Vitro Anticancer Drug Discovery Screen

Boyd, Michael R.

doi:10.1007/978-1-4615-8152-9_2

Cited by 221 publications

(223 citation statements)

References 25 publications

Supporting

Mentioning

212

Contrasting

Unclassified

Order By: Relevance

“…The current study makes use of the NCI-60 panel that consists of 60 diverse human cancer cell lines which have been used by the National Cancer Institute's Developmental Therapeutics Program to screen and profile >100,000 chemically defined compounds (plus a large number of natural product extracts) since 1990 (31,32). Included are 38 epithelial and 22 nonepithelial lines derived primarily from patients with advanced and/or metastatic disease.…”

Section: Introductionmentioning

confidence: 99%

Detailed DNA methylation profiles of the E-cadherin promoter in the NCI-60 cancer cells

Reinhold

Reimers

Maunakea

et al. 2007

Molecular Cancer Therapeutics

View full text Add to dashboard Cite

E-cadherin (E-cad) is a transmembrane adhesion glycoprotein, the expression of which is often reduced in invasive or metastatic tumors. To assess E-cad's distribution among different types of cancer cells, we used bisulfitesequencing for detailed, base-by-base measurement of CpG methylation in E-cad's promoter region in the NCI-60 cell lines. The mean methylation levels of the cell lines were distributed bimodally, with values pushed toward either the high or low end of the methylation scale. The 38 epithelial cell lines showed substantially lower (28%) mean methylation levels compared with the nonepithelial cell lines (58%). The CpG site at -143 with respect to the transcriptional start was commonly methylated at intermediate levels, even in cell lines with low overall DNA methylation. We also profiled the NCI-60 cell lines using Affymetrix U133 microarrays and found E-cad expression to be correlated with E-cad methylation at highly statistically significant levels. Above a threshold of f20% to 30% mean methylation, the expression of E-cad was effectively silenced. Overall, this study provides a type of detailed analysis of methylation that can also be applied to other cancer-related genes. As has been shown

show abstract

Section: Introductionmentioning

confidence: 99%

Detailed DNA methylation profiles of the E-cadherin promoter in the NCI-60 cancer cells

Reinhold

Reimers

Maunakea

et al. 2007

Molecular Cancer Therapeutics

View full text Add to dashboard Cite

show abstract

“…421 Ovarian cell lines and in vitro evaluation of growth inhibitory effects The activity of CJM 126, DF 129, DF 209, DF 229, DF 203, DF 180, DF 219, DF 221, DF 226 and NSC 680467 was examined in six ovarian cell lines (IGROV1, OVCAR-3, OVCAR-4, OVCAR-5, OVCAR-8 and SK-OV-3) in the laboratories of the NCI, Bethesda, MD, USA. The protocol adopted has been described in detail previously (Boyd, 1989;Monks et al, 1991Monks et al, , 1997 (Hamburger and Salmon, 1977 N,,N 5-(4-Dimethylaminophenylazo)quinoline (NSC 680467) Figure 1 Structures of 2-(4-aminophenyl)benzothiazoles and 5-(4-dimethylaminophenylazo)quinoline (NSC 680467) cultures were incubated for 7, 8 and 9 days respectively. Colony growth was monitored using an inverted microscope.…”

mentioning

confidence: 99%

Influence of 2-(4-aminophenyl)benzothiazoles on growth of human ovarian carcinoma cells in vitro and in vivo

Bradshaw

Shi²,

Schultz³

et al. 1998

Br J Cancer

View full text Add to dashboard Cite

Summary 2-(4-Aminophenyl)benzothiazole molecules substituted in the 3 position of the phenyl ring with a halogen atom or methyl moiety comprise a group of compounds that potently inhibit specific human ovarian carcinoma cell lines. G150 values fall within the nm range. Inhibition is highly selective -whereas the G150 value in IGROV1 cells consistently lies at < 10 nM, SK-OV-3 presents G150 values > 10jIM. Biphasic dose-response relationships were observed in sensitive cell lines after 48-h drug exposure. COMPARE analyses revealed the very similar profiles of anti-tumour activity of 3-substituted benzothiazoles and 5-(4-dimethylaminophenylazo)quinoline, with Pearson correlation coefficients > 0.65. Anti-tumour activity extended to preliminary in vivo tests. The growth of OVCAR-3 cells in polyvinylidene fluoride (PVDF) hollow fibres implanted in the peritoneal cavity of mice was inhibited by more than 50% after intraperitoneal (i.p

show abstract

“…This reflects their non-human derivation and relatively poor performance in identifying agents active against the more common solid tumours, e.g. breast, lung, colon (Boyd, 1989).…”

mentioning

confidence: 99%

“…The current screening at the National Cancer Institute (USA) uses an initial in vitro screen against a panel of human and animal cancer cell lines (Boyd 1989). Subsequent in vivo screens are performed against these cells growing in rodents.…”

mentioning

confidence: 99%