The nature of transcription selectivity of bacteriophage SPO1-modified RNA polymerase

Shub, David A.; Swanton, Marshal T.; Smith, Donna H.

doi:10.1007/bf00268282

Cited by 4 publications

(9 citation statements)

References 20 publications

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“…It has been demonstrated that the E. coli-coupled transcription-translation system used in this study results in the production of specific phage proteins from SPO1 DNA (21,25). The endogenous E. coli RNA polymerase and the purified B. subtilis RNA polymerase specify the same SPOt early proteins when SPOt DNA is used as a template in this system (22) (Fig. 4, lanes I and J) and compete for initiation sites on the template DNA (22).…”

mentioning

confidence: 73%

“…Preparation of RNA polymerase. RNA polymerase was prepared essentially according to Burgess and Jendrisak (2) as previously described (22). A Sephacryl S-200 column (3 by 30 cm) was included between the Polymin P (BASF Wyandotte Corp.) precipitation and DNA-cellulose chromatography as a sizing and desalting step.…”

Section: Geiduschek and S Okubomentioning

confidence: 99%

“…In vitro coupled transcription-translation. The E. coli-coupled system was prepared and incubations were performed as previously described (22). RNA synthesis was made dependent on added exogenous Rifr RNA polymerase by including rifampin in the reaction mixture.…”

Section: Geiduschek and S Okubomentioning

confidence: 99%

“…Three transcriptional control genes have been identified (8) whose products regulate the sequential expression of these transcripts. These genes encode polypeptides which bind tightly to the RNA polymerase and change its recognition specificity (5,22,26). Table 1 summarizes the time of appearance and genetic control over these classes of transcripts.…”

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confidence: 99%

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Transcriptional regulation of bacteriophage SPO1 protein synthesis in vivo and in vitro

Heintz¹,

Shub²

1982

J Virol

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There are six classes of SPOt transcripts which are, at least partially, regulated independently of each other. Analysis of proteins made in infections by phage mutants defective in DNA synthesis, or in genes which positively control transcription, permitted each protein to be assigned to one transcription class. Most of the late proteins belong to transcription class m21. There seem to be few, if any, phage proteins in the 1 class whose mRNA synthesis depends absolutely on phage DNA synthesis. UV irradiation of host cells allowed the detection of many additional early proteins. The early proteins detected in vivo were compared with proteins synthesized in vitro, using bacterial or gp28 phage-modified RNA polymerase in an Escherichia coli cell-free system. Proteins characterized in vivo as belonging to the e transcription class could be made efficiently in vitro only when transcription was performed by bacterial RNA polymerase. em proteins could be elicited through the use of either bacterial or gp28 polymerase, indicating that their genes can be transcribed in either the early or the middle mode.

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mentioning

confidence: 73%

Section: Geiduschek and S Okubomentioning

confidence: 99%

Section: Geiduschek and S Okubomentioning

confidence: 99%

mentioning

confidence: 99%

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Transcriptional regulation of bacteriophage SPO1 protein synthesis in vivo and in vitro

Heintz¹,

Shub²

1982

J Virol

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“…In vivo synthesis of SPOl middle RNA classes requires the modification of host RNA polymerase by the product of SPOl early gene 28 (gp28) (7). In vitro, we have used an E. coli-coupled transcription-translation cfs to study SPOl gene expression by placing SPOl RNA synthesis under the control of purified B. subtilis RNA polymerase isolated from uninfected or SPOl-infected rifampin-resistant cells (21). The procedure involves preincubation of SPOl DNA with Rif' B. subtilis RNA polymerase.…”

Section: Resultsmentioning

confidence: 99%

Mapping the genes in the terminal redundancy of bacteriophage SPO1 with restriction endonucleases

Perkus¹,

Shub²

1985

J Virol

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Although most early transcription from SPOl, a lytic DNA bacteriophage of Bacillus subtilis, is specified by the 12.6-kilobase region of the terminal redundancy, early genes from this region have not been identified by standard genetic means. We mapped genes to DNA regions of the SPOl terminal redundancy by analyzing in vitro protein synthesis from isolated SPOl restriction fragments in an Escherichia coli-coupled transcriptiontranslation cell-free system. DNA from the terminal redundancy directs the synthesis in vitro of eleven proteins, e3, e4, e6, e7, e9, e12, e15, e16, e18, e20, and e21, which correspond in mobility on sodium dodecyl sulfate-polyacrylamide gels with authentic SPOl early proteins. From their mapped positions on the DNA, genes were positioned downstream from most, but not all, of the twelve early promoter regions identified in vitro in the terminal redundancy. The temporal patterns of early protein synthesis in vivo suggest a differential turning on and off of early promoters in the terminal redundancy. Both in vivo and in vitro evidence suggests the existence of previously unidentified early promoter regions upstream from the genes for e6 and e4 as well as a middle promoter region upstream from the gene for e16.

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Bacteriophage SPO1

Stewart¹

1988

The Bacteriophages

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The nature of transcription selectivity of bacteriophage SPO1-modified RNA polymerase

Cited by 4 publications

References 20 publications

Transcriptional regulation of bacteriophage SPO1 protein synthesis in vivo and in vitro

Transcriptional regulation of bacteriophage SPO1 protein synthesis in vivo and in vitro

Mapping the genes in the terminal redundancy of bacteriophage SPO1 with restriction endonucleases

Bacteriophage SPO1

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