The nature of some protein microenvironmental effects

Williamson, Charles E.; Corwin, Alsoph H.

doi:10.1016/0021-9797(72)90391-8

Cited by 14 publications

(10 citation statements)

References 27 publications

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“…Also included in table 2 are the pK," values for the azine derivatives in these micellar systems. Eqn (10) gives the relationship between the pKzbS value of an azine derivative residing within a charged micellar interfacial region and its pKZ value.…”

Section: Resultsmentioning

confidence: 99%

Acid–base equilibria in aqueous micellar solutions. Part 3.—Azine derivatives

Drummond¹,

Grieser²,

Healy³

1989

J. Chem. Soc., Faraday Trans. 1

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The acid-base behaviour of two azine derivatives, viz. neutral red and acridine, in aqueous non-ionic Brij-35 and n-octyl 8-D-glucoside micellar solutions, in aqueous anionic sodium dodecyl sulphate micellar solutions and in 1,6dioxane-water mixtures have been investigated. The factors primarily responsible for the difference between the pK, value of an azine derivative in pure water and its apparent pK, value when it is located quantitatively within the micelle-aqueous solution interface have been determined. For the non-ionic micellar systems, the difference can be explained solely in terms of the different intrinsic solvent properties of the two solvating media. For the anionic micellar systems three major factors are considered to be responsible for the difference. These factors are the electrostatic micellar surface potential, the interfacial solvent characteristics, and specific molecular interaction between the cationic protonated moieties of the azine derivatives and the anionic surfactant headgroups.log ([H+] y:) -= B+ log VH(3) where log WH is a correction factor discussed in Part 2 of this series.17

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Section: Resultsmentioning

confidence: 99%

Acid–base equilibria in aqueous micellar solutions. Part 3.—Azine derivatives

Drummond¹,

Grieser²,

Healy³

1989

J. Chem. Soc., Faraday Trans. 1

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“…Indicator equilibria provided the first examples of micellar-induced effects upon chemical reactions in aqueous solution (1). The dye neutral red has been used to gain insight into the characteristics of microenvironments of normal micelles (2)(3)(4), membranes (5), and proteins (6). The study of the acid-base behavior of dye(s) has found importance for chemical studies in micelles and microemulsions (7).…”

Section: Introductionmentioning

confidence: 99%

Effects of Nonionic, Cationic, and Mixed Nonionic–Cationic Surfactants on the Acid–Base Behavior of Phenyl Salicylate

Khan

Arifin

Yusoff

et al. 1999

Journal of Colloid and Interface Science

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“…The high correlation between spectral changes in mixed aqueous solvents and in hydrophobic binding sites suggests that the dyes experience similar changes in environment in both cases. This correlation has led some investigators to interpret the "polarity" of protein binding sites in terms of empirical solvent polarity parameters (9,14,20). Our recent findings that some hydrophobic dyes undergo specific solvation changes in mixed aqueous solvents that are related to changes in solvent structure (21,22) has led us to make a detailed spectral analysis of MO and similar dyes in pure hydrogen-bonding solvents, in aqueous binaries, in a surfactant micelle, and bound in albumin.…”

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confidence: 99%

Analysis of the Visual Spectrum of Methyl Orange in Solvents and in Hydrophobic Binding Sites

Reeves¹,

Kaiser²,

Maggio³

et al. 1973

Can. J. Chem.

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The absorption curves of methyl orange (MO) and analogous p-aminophenylazobenzenes in organic and aqueous organic solvents are shown to consist of two severely overlapping bands. The curves have been resolved into two skewed component bands using a regression method. The blue shift of the absorption maximum obtained when organic solvents are added to aqueous solutions of MO, or when MO is bound to bovine serum albumin or a surfactant micelle, is the result of a change in relative intensities of the component bands. The low-frequency component is assigned to a n, -. nlx transition of a solvate in which there is specific hydrogen-bonding interaction between solvent and the azo nitrogens, and the high-frequency component to a n1 + nl* transition of a solvate in which the interaction is absent. The low-frequency component is favored by aqueous solvent compositions and by low temperatures. The free energies of interconversion of the species in various hydrogen-bonding solvents are correlated by the solvent surface tension but not by the dielectric constant. The results show that the shift in absorption maximum accompanying binding to a protein or micelle should be interpreted as a shift in an equilibrium rather than as a shift in transition energy.Les courbes d'absorption du methylorange (MO) et des p-aminophCnylazobenzenes analogues, en solvants organiques et organiques aqueux, comprennent deux bandes fortement enchevktrees. La resolution de ces courbes en deux bandes gauches elementaires a ete faite par la methode de regression. Le d6placement vers le bleu du nlaxilnuin d'absorption, obtenu par addition de solvants organiques aux solutions aqueuses de MO ou lorsque le MO est lie a l'albumine du serum bovin ou a une micelle surfactive, est le resultat du changement des intensites relatives des bandes composantes. La bande a basse frequence est attribue a la transition n, + nl*, d'un produit de solvatation dans lequel il y a interaction specifique par liaison hydrogene entre le solvant et les azotes de l'azo; la bande a haute frequence correspond a la transition nl + n," du produit de solvatation sans interaction. La bande a basse frequence est favorisee par la composition en eau des solvants et aux basses temperatures. Les energies libres de l'interconversion des diverses especes dans differents solvants a liaison hydrogene, ont ete reliies a la tension superficielle du solvant et non a la constante dielectrique. Les rtsultats montrent que le deplacement du maximum d'absorption consecutif a une liaison a une protkine ou ~nicelle devraient &tre interpretes pluttit comme un deplacement d'equilibre qu'un diplacement de 1'Cnergie de transition.[Traduit par le journal]Can. J. Chem.. 51. 628 (1973) Methyl orange (MO, 1) has been used as a probe for studying the interaction of organic anions with proteins (1-9), synthetic polymers (10, 1 I), polyelectrolytes (12), and surfactants (12-14) in aqueous solution. The interaction is usually accompanied by spectral changes that are not associated with the acid-base indicator p...

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The nature of some protein microenvironmental effects

Cited by 14 publications

References 27 publications

Acid–base equilibria in aqueous micellar solutions. Part 3.—Azine derivatives

Acid–base equilibria in aqueous micellar solutions. Part 3.—Azine derivatives

Effects of Nonionic, Cationic, and Mixed Nonionic–Cationic Surfactants on the Acid–Base Behavior of Phenyl Salicylate

Analysis of the Visual Spectrum of Methyl Orange in Solvents and in Hydrophobic Binding Sites

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