The Nature of Carbonic Anhydrase From Plant Sources

Sibly, Pamela M; Wood, J.F.

doi:10.1071/bi9510500

Cited by 11 publications

(6 citation statements)

References 4 publications

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“…Upon standing in the absence of cysteine the enzyme rapidly lost activity. This observation was supported by later evidence that p-chloromercuribenzoate (PCMB), iodobenzoate, and azide, all fairly specific inactivators of sulfhydryl groups, inhibit enzymatic activity in a number of different kinds of plants (Bradfield, 1947;Everson, 1970;Kiesel and Graf, 1972;Sibly and Wood, 1951).…”

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confidence: 80%

Carbonic anhydrase levels and internal lacunar CO/sub 2/ concentrations in aquatic macrophytes

Weaver¹

1979

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Carbonic anhydrase levels were examined in a variety of aquatic macrophytes from different habitats. In general, carbonic anhydrase levels increased across the habitat gradient such that activities were low in submersed aquatic macrophytes and high in emergent macrophytes with floatingleaved and free-floating plants exhibiting intermediate activities. Internal lacunar C0~ concentrations were analyzed in relation to carbonic anhydrase activities. There was no correlation between these two parameters. Internal C0» concentrations ranged from low to high in submersed macrophytes, but were low in floating-leaved and emergent macrophytes. The observed internal CO-concentrations are discussed in relation to the individual morphologies of the plants and the environments in which they occurred.

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confidence: 80%

Carbonic anhydrase levels and internal lacunar CO/sub 2/ concentrations in aquatic macrophytes

Weaver¹

1979

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“…Furthermore. Bradfie'ld (1947) and Sibly and Wood (1951} found that sulfanilamide at a concentration 10~^ M caused litlle or no inhibition of the aclivity of the land plant enzyme obtained from Iwo difffrenl species. The enzyme obtained from Vlmi pertusa would appear lo be different from land plants, however, since its activily is completely inhibited by low concentrations of sulfaniiamide and Diamox as is seen in Figures 3 and 4.…”

Section: Inhibitory Effects Of Siilfanilamide and Diamox On Enzyme Acmentioning

confidence: 99%

Carbonic Anhydrase in the Marine Alga Ulva pertusa

Ikemori

Nishida

1968

Physiologia Plantarum

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The preparation of stable samples containing carbonic anhydrase from Ulva pertusa was achieved by using artificial sea water and 10 mM cysteine solution adjusted to pH 8.2 instead of 10 mM cysteine solution alone. When the sample was stored at 0°C in darkness the enzyme activity was retained for about 24 h. In contrast to the results obtained with land plants in previous works a remarkable inhibitory effect of low concentrations of sulfanilamide and Diamox (2‐acetylamino‐1,3,4‐thiadiazole‐5‐sulfonamide) on the activity of the enzyme was observed.

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“…Plant carbonic anlhydrase is thought to be only wveaklx affected, if at all, by sulfonamides (5,9) although the literature shows s,ome variability (1,11,13,19,24). It has been found by some workers that free sulfhydryl groups are necessarv for expression of the plant enzyme or for its enhancement (1,23) but variability in this regard has ailso been reported (15,24).…”

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confidence: 99%