The Nature of Antibodies and Antigens

Binding properties of a mouse immunoglobulin M myeloma protein with carbohydrate specificity

“…The association constant for the binding of nigerosyl-a(l->-4)-nigerose by MOPC 104E IgM, 3.6 X 104 at 4°, falls in the lower end of the range 104 to 109 recorded for antibody affinities (Eisen and Pearce, 1962). Carbohydrate antibodies in general have association constants below 5 X 105 (Table II), and in fact the MOPC 104E IgM has a higher association constant than the rabbit IgM antibody to S. typhi.…”

Studies in Immunoglobulin Chemisty. I. Reinvestigation of the C-Terminal Amino Acids of 6.5S Rabbit and Human γ-Globulins*

“…T A. he nature of the Cand N-terminal amino acids of 7-globulin has been the subject of numerous investigations (reviewed by Eisen and Pearce, 1962;Porter and Press, 1962). In retrospect, most of these end-group determinations have failed to account satisfactorily, in terms of molar quantities, with the four-chain polypeptide structure (Fleischman et al, 1962(Fleischman et al, , 1963; Edelman and Poulik, 1961) which has emerged for IgG.1…”