The nature and features of organization of reserve polysaccharides in three Pelomyxa species (Archamoebea, Pelobiontida)

Pelomyxa doughnuta sp. nov. (Archamoebea, Pelobiontida) with an unusual nucleus‐glycogen association

“…Glycogen is present in the cytoplasm of most Pelomyxa spp. (Chistyakova, Bezborodkina, et al, 2020). In some species ( P .…”

“…corona , P . stagnalis ), glycogen accumulations in cytoplasm are represented by discrete organelle‐like rounded or oval bodies, the so‐called glycogen bodies, usually surrounded by small ER vesicles (Chistyakova, Bezborodkina, et al, 2020; Chistyakova & Frolov, 2011; Frolov, Chystjakova, et al, 2005 Frolov et al, 2004). Here, we showed that perinuclear glycogen sheath of the Pelomyxa sp.…”

“…Glycogen was detected with the help of a fluorescent PAS (Periodic Acid‐Schiff) reaction with Auramine‐SO 2 (Au‐SO 2 ) according to a previously described technique (Chistyakova, Bezborodkina, et al, 2020). The specificity of PAS reaction was checked by treating the preparations with α‐amylase solution.…”

“…A characteristic feature of the biology and morphology of most Pelomyxa spp. is the presence of significant amounts of glycogen, a reserve polysaccharide, in their cytoplasm (Chistyakova, Bezborodkina, et al, 2020). At the same time, different species of pelomyxae differ markedly in the distribution and organization of glycogen accumulations.…”

“…At the same time, different species of pelomyxae differ markedly in the distribution and organization of glycogen accumulations. In many cases, these accumulations form special organelle‐like cytoplasmic structures, the so‐called glycogen bodies (Andresen et al, 1968; Chistyakova, Bezborodkina, et al, 2020; Daniels, 1973; Frolov, 2011).…”