The Nature and Composition of Experimental Bacterial Aerosols1

Kethley, T. W.; Cown, W. B.; Fincher, E. L.

doi:10.1128/aem.5.1.1-8.1957

Cited by 14 publications

(11 citation statements)

References 18 publications

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“…Several investigators (Kethley et al, 1957;DeOme et al, 1944;Webb, 1959;Hayakaw and Poon, 1965) have determined and discussed the influence of temperature and relative humidity on viability of bacterial aerosols.…”

Section: Resultsmentioning

confidence: 99%

“…5. Death rate constants for aerosols of S. Ylzarcescens were determined by Kethley et al (1957) The negative temperature and relative humidity gradients produced experimental results which were low due to adverse conditions existing as the aerosol moved from the high to low concentration space. The positive gradients produced experimental coefficients which were high due to the less severe conditions existing as the aerosol moved from the high to the low concentration space.…”

Section: Resultsmentioning

confidence: 99%

“…Influence of temperature gradient on transfer coeficient. I Influence of temperature and relative humidity on death rate constants of S. marcescens(Kethley et al, 1957).…”

mentioning

confidence: 99%

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Factors Influencing the Transport of Air‐Borne Bacteria at Openings

Heldman

Hedrick

Hall

1967

Journal of Food Science

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SUMMARY— The factors causing transport of air‐borne microorganisms from a high concentration space to a low concentration space were investigated in an aerosol chamber. The transport of bacterial aerosol through an opening between the compartments of the chamber was expressed in terms of a turbulent transfer coefficient. The experimental transfer coefficients increased significantly as the ventilation rates increased from 20 to 40 ft3/min and increased slightly between 40 and 60 ft3/min. Results obtained with ventilation rate gradients ranging from − 30 to +30 ft3/min indicated that the coefficient was maximum at equal rates. An increase in the opening height from 3 to 9 in. produced a significant decrease in the turbulent transfer coefficient. Temperature gradients ranging from −14 to +12S°F with respect to a reference temperature of 75°F resulted in an increase in the turbulent transfer coefficients from 2.09 to 8.09. The effect of temperatures between 75 and 95°F and corresponding relative humidities between 31 and 60%, on aerosol viability did not account for the entire influence of the temperature gradient on aerosol transport.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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Factors Influencing the Transport of Air‐Borne Bacteria at Openings

Heldman

Hedrick

Hall

1967

Journal of Food Science

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“…Airborne characterization of a stabilized culture was determined by the response of the airborne organisms atomized directly from a beef extract broth culture into the standard atmosphere of 20 C and 65 per cent relative humidity. Procedures are described in detail in earlier publications (Kethley et al, 1956(Kethley et al, , 1957).…”

Section: Methodsmentioning

confidence: 99%

Variations in the Aerial Viability Associated with Variation in Morphology of Color Variants of Serratia marcescens1

Fincher¹,

Kethley²,

Cown³

1957

Applied Microbiology

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“…introduced into either a cubical or cylindrical chamber system. The production of the test aerosol and the details of the chamber system -are described in detail elsewhere (Kethley et al, 1956;Kethley et al, 1957). Critical-orifice liquid impinger samplers.…”

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confidence: 99%

The Critical-Orifice Liquid Impinger as a Sampler for Bacterial Aerosols1

Cown¹,

Kethley²,

Fincher³

1957

Applied Microbiology

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Studies in aerobiology are often conducted on a comparative basis; that is, the numbers of viable airborne organisms obtained under one set of circumstances are compared to those obtained under other conditions. For samplers employed in such studies, reproducibility and ease of handling often are considered more important than absolute efficiency because the final conclusions are derived from internal comparisons between various data collected using the same samplers throughout. In planning studies at Georgia Institute of Technology on the effect of chemical vapors on the viability of airborne bacteria, the critical-orifice liquid impinger was selected because of the demonstrated reproducibility and ease of handling (Rosebury 1947; Henderson 1952). Subsequent experience justified this choice, although it was found that the efficiency was significantly affected by the composition of the liquid used in the sampler. Further studies showed that this effect was responsible for potentially large errors when estimating particle size by the method of comparing aerial concentration to number settling per unit area per time unit. This experience indicated the need for consideration of other factors which might influence the operation of critical-orifice liquid impingers when used as samplers for bacterial aerosols. MATERIALS AND METHODS The test culture. The investigations were carried out with carefully differentiated cultures of Serratia marcescens strain ATCC 274, maintained in 0.3 per cent beef extract broth. The cultures were 45 to 48 hr old when used in tests (Kethley et al., 1956). Chambers and equipment for the production and maintenance of the bacterial aerosol. The organisms were

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The Nature and Composition of Experimental Bacterial Aerosols1

Cited by 14 publications

References 18 publications

Factors Influencing the Transport of Air‐Borne Bacteria at Openings

Factors Influencing the Transport of Air‐Borne Bacteria at Openings

Variations in the Aerial Viability Associated with Variation in Morphology of Color Variants of Serratia marcescens1

The Critical-Orifice Liquid Impinger as a Sampler for Bacterial Aerosols1

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