The natural history of liver regeneration in rats: Description of an animal model for liver regeneration studies

Andersen, Kasper Jarlhelt; Knudsen, Aage; Kannerup, Anne-Sofie; Sasanuma, Hideki; Nyengaard, Jens Randel; Hamilton-Dutoit, Stephen; Erlandsen, Erland J.; Jørgensen, Bo; Mortensen, Frank Viborg

doi:10.1016/j.ijsu.2013.07.009

Cited by 35 publications

(26 citation statements)

References 20 publications

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“…In total, 104 male Wistar rats were randomized in blocks of 5 according to the size of partial hepatectomy to be performed: PH (30) (n = 24), PH (70) (n = 24), PH (90) (n = 24), sham (n = 24) and baseline (n = 8). Each group (not baseline) was further block‐randomized into 3 subgroups according to the day of euthanization: postoperative days (PODs) 1, 3, and 5 (n = 8 per group)(Figure ) in accordance with a previous study conducted by this study group presenting the natural history of liver regeneration in rats subjected to PH (70) PH …”

Section: Methodsmentioning

confidence: 99%

Adaptive growth changes in the liver remnant are affected by the size of hepatectomy in rats

Meier

Andersen

Knudsen

et al. 2018

Int J Experimental Path

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In this study we investigated the dynamics of hepatocyte hyperplasia and hypertrophy in rats subjected to increasing sizes of partial hepatectomy (PH). A total of 104 rats were randomized according to the size of PH. On postoperative days (PODs) 1, 3 and 5, blood was drawn and the remnant liver removed for stereological analysis. Liver parameters and regeneration rate were significantly affected by size of PH. On POD 1, hepatocyte volumes had increased significantly in all PH groups. On POD 3, all groups showed hepatocyte volumes approximating baseline. On POD 5, hepatocyte volumes were significantly lower in PH (90) than in baseline, sham and PH (30) rats. Increasing hepatocyte proliferation was not observed following PH (30). Following PH (70), cell proliferation was significantly elevated on PODs 1 and 3, and following PH (90) on PODs 3 and 5. In conclusion, general hypertrophy of hepatocytes after different size of PH was followed by hepatocyte proliferation only in the liver remnant of PH (70) and PH (90).

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Section: Methodsmentioning

confidence: 99%

Adaptive growth changes in the liver remnant are affected by the size of hepatectomy in rats

Meier

Andersen

Knudsen

et al. 2018

Int J Experimental Path

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“…The binding sites of antibodies were visualized with DAB (Sigma). Negative control slides in the absence of primary antibody were included for each staining [34].…”

Section: Histopathologic Examinationmentioning

confidence: 99%

Preventive effects of quercetin on liver damages in high-fat diet-induced obesity

Gedikli¹,

Özkanlar²,

Gür³

et al. 2017

J Histol Histopathol

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Background: Obesity is a worldwide health problem and causes many important illnesses. The current study aimed to investigate the influence of quercetin over apoptosis by inflammatory activity on high-fat dietinduced liver damage. Material and methods: Totally 18 adult female Sprague-Dawley rats were selected and randomly separated equally into three groups, followed as control, obesity and obesity-quercetin groups. Obesity and obesityquercetin groups were fed a moderately high fat diet for 120 days. After obesity occurred, quercetin dissolved in the corn oil and given obesity-quercetin group at dose of 50 mg/kg by oral gastric way for 15 days. All animals were sacrificed with an anesthesia at the end of the experiment, and venous blood samples were collected from the right ventricle and hepatic markers were evaluated by using commercially available diagnostic kits. In addition, liver sections were analysed by histopathologic and immunohistochemical procedures. Also, we evaluated the expression of Bax and Bcl-2 by immunohistochemistry. Results: Obesity induced important change (p<0.05) in the most of biochemical parameters, hepatic markers, and Bax immunoreactivity as well. But, the level of Bcl-2 was slightly increased, in contrast, the level of Bax decreased markedly (p<0.05) in the obesity-quercetin group. Conclusions: Both immunohistochemical evidence and biochemical results showed that application of quercetin decreased the obesity mediated damage to the liver.

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“…Yet, some animals developed the ability to regenerate, for example amphibian limbs and lizard tails . Mammals have limited ability to regenerate, but studies have shown examples of mammalian regeneration including ear punch hole closure, wound‐induced hair neogenesis (WIHN), liver regeneration after injury and digit tip regeneration . Inbred strains of laboratory mice demonstrate hair follicle regeneration after full‐thickness (FT) wounding, mixed lineage strains show enhanced regeneration and wild African Spiny mice exhibit dorsal skin regeneration even after 60% removal due to FT injury .…”

Section: Introductionmentioning

confidence: 99%

Regeneration of rete ridges in Lanyu pig (Sus scrofa): Insights for human skin wound healing

Lin

Chiu

Hsueh

et al. 2019

Experimental Dermatology

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Rete ridges are important to the mechanical function of skin in animals with minimal hair, including humans. As mice do not exhibit rete ridges, the need for a quality animal model is pertinent. Here, we develop a Lanyu pig (Sus scrofa) full‐thickness wound model to explore tissue regeneration because the architecture and function are similar to humans and inbred genetic variants are available. Full‐ and partial‐thickness wounds were generated on the dorsum. Full‐thickness wounds at post‐wound day 57 exhibit severe scar with no signs of wound‐induced hair follicle neogenesis. Wound contraction is greater in the anterior/posterior relative to the medial/lateral axis. In wound beds, K14+ cells increased while K10+, p63+ and PCNA+ cells decreased compared to unwounded tissue. Epithelial β‐catenin is unchanged. The wound bed expresses more ColI, less ColIII and no elastin. Rete ridges do not form after full‐thickness wounding, but incompletely regenerate after partial‐thickness wounding. An alkaline phosphatase (ALP)+ cell population, not associated with hair follicles, is present at the bottom of the rete ridge basal layer in pig and human unwounded skin. These K5+/K10−/PCNA−/ALP+ epithelial cells are absent after full‐thickness wounding but reappear after partial‐thickness wounding, before invagination of new rete ridges. In summary, full‐thickness wounding on the dorsum of Lanyu pigs results in scar formation and perturbed molecular expression while partial‐thickness wounding permits limited rete ridge and papillary dermis regeneration. Future functional studies and further characterization will help contribute knowledge for the regenerative medicine field.

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The natural history of liver regeneration in rats: Description of an animal model for liver regeneration studies

Abstract: Seventy percent partial hepatectomy in healthy rats induces a rapid regenerative response and PODs 2, 4 and 8 seems optimal for assessing hepatic growth in future studies.

Cited by 35 publications

References 20 publications

Adaptive growth changes in the liver remnant are affected by the size of hepatectomy in rats

Adaptive growth changes in the liver remnant are affected by the size of hepatectomy in rats

Preventive effects of quercetin on liver damages in high-fat diet-induced obesity

Regeneration of rete ridges in Lanyu pig (Sus scrofa): Insights for human skin wound healing

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