The nasal microbiota in infants with cystic fibrosis in the first year of life: a prospective cohort study

Mika, Moana; Korten, Insa; Qi, Weihong; Regamey, Nicolas; Frey, Urs; Casaulta, Carmen; Latzin, Philipp; Hilty, Markus

doi:10.1016/s2213-2600(16)30081-9

Cited by 68 publications

(72 citation statements)

References 31 publications

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“…Nevertheless, a significant effect of season on the microbiota was observed in the human-derived sample types from pig and cow farmers but not nonexposed individuals. In young children and adults, it has also been shown that season acts as a major factor in driving the dynamics of the nasal microbiota (25,28). In contrast to our findings, De Boeck et al reported no correlation between season and microbial composition in healthy humans (29).…”

Section: Discussioncontrasting

confidence: 99%

The Indoor-Air Microbiota of Pig Farms Drives the Composition of the Pig Farmers’ Nasal Microbiota in a Season-Dependent and Farm-Specific Manner

Kraemer

Aebi

Oppliger

et al. 2019

Appl Environ Microbiol

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Prior studies have demonstrated an influence of the built environment on the human nasal microbiota. However, very little is known about the influences of working on a pig farm on the human nasal microbiota. We longitudinally collected samples from 30 pig farms (air and nasal swabs from humans and pigs) in Switzerland from 2014 to 2015. As controls, nasal swabs from cow farmers and individuals with no contact with farm animals were included. An analysis of the microbiota for all samples (n = 609) was performed based on 16S rRNA gene sequencing (MiSeq) and included the investigations of source-sink dynamics. The numbers of indoor airborne particles and bacterial loads in pig farms were also investigated and were highest in winter. Similarly, the microbiota analyses revealed that the alpha diversity values of the nares of pig farmers were increased in winter in contrast to those of samples from the nonexposed controls, which displayed low alpha diversity values throughout the seasons. Source-sink analyses revealed that bacteria from the noses of pigs are more commonly coidentified within the pig farmers' microbiota in winter but to a less extent in summer. In addition, in winter, there was a stronger intrasimilarity for samples that originated from the same farm than for samples from different farms, and this farm specificity was partially or completely lost in spring, summer, and fall. In conclusion, in contrast to nonexposed controls, a pig farmer’s nasal microbiota is dynamic, as the indoor-air microbiota of pig farms drives the composition of the pig farmer’s nasal microbiota in a season-dependent manner. IMPORTANCE The airborne microbiota of pig farms poses a potential health hazard and impacts both livestock and humans working in this environment. Therefore, a more thorough understanding of the microbiota composition and dynamics in this setting is needed. This study was of a prospective design (12 months) and used samples from different sites. This means that the microbiota of air, animals (pigs), and humans was simultaneously investigated. Our findings highlight that the potential health hazard might be particularly high in winter compared to that in summer.

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Section: Discussioncontrasting

confidence: 99%

The Indoor-Air Microbiota of Pig Farms Drives the Composition of the Pig Farmers’ Nasal Microbiota in a Season-Dependent and Farm-Specific Manner

Kraemer

Aebi

Oppliger

et al. 2019

Appl Environ Microbiol

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“…It does, however, contrast with the findings of previous longitudinal analyses of children with CF, which only examined the upper airway microbiota 10 28–32. During infancy, the nasopharyngeal microbiota is dynamic,33–35 although differences between that of infants with CF and healthy controls have been reported,30 31 including increased relative abundance of Staphylococcae at baseline,31 changes in Staphylococcus species,31 and the emergence of gram-negative bacteria30 following antibiotic exposure. Two smaller studies examining oropharyngeal swabs from infants29 and children10 with CF suggested that upper airway microbial diversity increased with age.…”

Section: Discussionmentioning

confidence: 99%

The lower airway microbiota in early cystic fibrosis lung disease: a longitudinal analysis

Frayman

Armstrong

Carzino

et al. 2017

Thorax

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“…Genetic diseases affecting mucociliary clearance, such as cystic fibrosis and primary ciliary dyskinesia, affect the URT microbiota. Two recent studies have identified that infants with cystic fibrosis, which results in a thickened mucus layer, have an altered URT microbiota development compared to healthy infants including increased relative abundance of Staphylococcus species and decreased abundance of potential beneficial bacteria . Many people with primary ciliary dyskinesia, a combination of diseases resulting in decreased ciliary action to clear mucus, also suffer from chronic rhinosinusitis, and there is a high reported rate of URT manifestations of disease, contributing to morbidity .…”

Section: Factors Affecting Urt Microbiotamentioning

confidence: 99%

Composition and immunological significance of the upper respiratory tract microbiota

2016

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The intestinal microbiota is essential for nutrient acquisition, immune development, and exclusion of invading pathogens. The upper respiratory tract (URT) microbiota is less well studied and does not appear to abide by many of the paradigms of the gastrointestinal tract. Decades of carriage studies in children have demonstrated that microbe-microbe competition and collusion occurs in the URT. Whether colonization with common pathogens (e.g., Staphylococcus aureus and Streptococcus pneumoniae) alters immune development or susceptibility to respiratory conditions is just beginning to be understood. Herein, we discuss the biogeography of the URT microbiota, the succession and evolution of the microbiota through the life course, and discuss the evidence for microbe-microbe interactions in colonization and infection.

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The nasal microbiota in infants with cystic fibrosis in the first year of life: a prospective cohort study

Abstract: Swiss National Science Foundation, Fondation Botnar, the Swiss Society for Cystic Fibrosis, and the Swiss Lung Association Bern.

Cited by 68 publications

References 31 publications

The Indoor-Air Microbiota of Pig Farms Drives the Composition of the Pig Farmers’ Nasal Microbiota in a Season-Dependent and Farm-Specific Manner

The Indoor-Air Microbiota of Pig Farms Drives the Composition of the Pig Farmers’ Nasal Microbiota in a Season-Dependent and Farm-Specific Manner

The lower airway microbiota in early cystic fibrosis lung disease: a longitudinal analysis

Composition and immunological significance of the upper respiratory tract microbiota

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