The NAC transcription factor ANAC046 is a positive regulator of chlorophyll degradation and senescence in Arabidopsis leaves

Oda-Yamamizo, Chihiro; Mitsuda, Nobutaka; Sakamoto, Shingo; Ogawa, Daisuke; Ohme‐Takagi, Masaru; Ohmiya, Akemi

doi:10.1038/srep23609

Cited by 139 publications

(109 citation statements)

References 61 publications

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“…Many previous studies have identified the TFs responsible for controlling Chl metabolism in leaves (Lim, 2003; Lin et al, 2015; Wen et al, 2015). Three TFs, ANAC046, ETHYLENE INSENSITIVE3, and ORE1, positively regulate Chl degradation by binding to the promoter regions of NYC, SGR1 , and PaO (Qiu et al, 2015; Oda-Yamamizo et al, 2016). FLU, a negative regulator of Chl biosynthesis in A. thaliana , may mediate its regulatory effect through interaction with enzymes (GSA and CHlH) involved in Chl biosynthesis (Meskauskiene et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

Spatiotemporal Transcriptome Analysis Provides Insights into Bicolor Tepal Development in Lilium “Tiny Padhye”

Yang²,

Feng

et al. 2017

Front. Plant Sci.

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The bicolor Asiatic hybrid lily cultivar “Tiny Padhye” is an attractive variety because of its unique color pattern. During its bicolor tepal development, the upper tepals undergo a rapid color change from green to white, while the tepal bases change from green to purple. However, the molecular mechanisms underlying these changes remain largely uncharacterized. To systematically investigate the dynamics of the lily bicolor tepal transcriptome during development, we generated 15 RNA-seq libraries from the upper tepals (S2-U) and basal tepals (S1-D, S2-D, S3-D, and S4-D) of Lilium “Tiny Padhye.” Utilizing the Illumina platform, a total of 295,787 unigenes were obtained from 713.12 million high-quality paired-end reads. A total of 16,182 unigenes were identified as differentially expressed genes during tepal development. Using Kyoto Encyclopedia of Genes and Genomes pathway analysis, candidate genes involved in the anthocyanin biosynthetic pathway (61 unigenes), and chlorophyll metabolic pathway (106 unigenes) were identified. Further analyses showed that most anthocyanin biosynthesis genes were transcribed coordinately in the tepal bases, but not in the upper tepals, suggesting that the bicolor trait of “Tiny Padhye” tepals is caused by the transcriptional regulation of anthocyanin biosynthetic genes. Meanwhile, the high expression level of chlorophyll degradation genes and low expression level of chlorophyll biosynthetic genes resulted in the absence of chlorophylls from “Tiny Padhye” tepals after flowering. Transcription factors putatively involved in the anthocyanin biosynthetic pathway and chlorophyll metabolism in lilies were identified using a weighted gene co-expression network analysis and their possible roles in lily bicolor tepal development were discussed. In conclusion, these extensive transcriptome data provide a platform for elucidating the molecular mechanisms of bicolor tepals in lilies and provide a basis for similar research in other closely related species.

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Section: Discussionmentioning

confidence: 99%

Spatiotemporal Transcriptome Analysis Provides Insights into Bicolor Tepal Development in Lilium “Tiny Padhye”

Yang²,

Feng

et al. 2017

Front. Plant Sci.

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“…Because the nongreen seed phenotype increased with prolonged silique exposure to light, it was suggested that the phenotype may be due to a photodynamic effect on the accumulating Chl compounds (Li et al, 2017). AdMYB7 activation of SGR1 to promote Chl degradation would be consistent with the transgenic phenotypes that showed significantly reduced Chl concentrations and would be similar to TFs, such as NAC016 and ANAC046, which activate the promoters of Chl catabolic enzymes (Sakuraba et al, 2012;Oda-Yamamizo et al, 2016).…”

Section: New Phytologistmentioning

confidence: 98%

“…This phenotype suggested MYB7 may cause Chl degradation and/or interfere with plastid development, which is consistent with the upregulation of NbSGR1 and the consequent downregulation of genes associated with chloroplast and thylakoid membrane organization. SGR1 promotes Chl degradation by interacting with, and probably recruiting, Chl catabolic enzymes during senescence (Sakuraba et al, 2012(Sakuraba et al, , 2015Oda-Yamamizo et al, 2016). SGR proteins have magnesium-de-chelatase activity, responsible for the extraction of magnesium from Chla and converting it to pheophytin a, during Chl degradation (Shimoda et al, 2016).…”

Section: New Phytologistmentioning

confidence: 99%

A kiwifruit (Actinidia deliciosa) R2R3‐MYB transcription factor modulates chlorophyll and carotenoid accumulation

et al. 2018

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MYB transcription factors (TFs) regulate diverse plant developmental processes and understanding their roles in controlling pigment accumulation in fruit is important for developing new cultivars. In this study, we characterised kiwifruit TFMYB7, which was found to activate the promoter of the kiwifruit lycopene beta-cyclase (AdLCY-β) gene that plays a key role in the carotenoid biosynthetic pathway. To determine the role of MYB7, we analysed gene expression and metabolite profiles in Actinidia fruit which show different pigment profiles. The impact of MYB7 on metabolic biosynthetic pathways was then evaluated by overexpression in Nicotiana benthamiana followed by metabolite and gene expression analysis of the transformants. MYB7 was expressed in fruit that accumulated carotenoid and Chl pigments with high transcript levels associated with both pigments. Constitutive over-expression of MYB7, through transient or stable transformation of N. benthamiana, altered Chl and carotenoid pigment levels. MYB7 overexpression was associated with transcriptional activation of certain key genes involved in carotenoid biosynthesis, Chl biosynthesis, and other processes such as chloroplast and thylakoid membrane organization. Our results suggest that MYB7 plays a role in modulating carotenoid and Chl pigment accumulation in tissues through transcriptional activation of metabolic pathway genes.

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“…As illustrated in Supplemental Figure S3, the phylogenetic analysis classified the NACs into 14 groups, designated as Roman numeral families (NAC-I to NAC-XIV) based on tree topologies. The published senescence-associated NACs (Kim et al, 2016;Liang et al, 2014;Nakashima et al, 2007;Oda-Yamamizo et al, 2016;Sperotto et al, 2009) fall in groups I to IV. In detail, ANAC053, ANC016, ANAC017, and ANAC096 located in NAC II, ANAC083, and ONAC106 located in NAC III, while ANAC042, ONAC058 (OsNAP), AtNAP, ANAC047, ANAC019, AtNAC3, OsNAC5, ATAF1, and OsNAC5 located in NAC IV.…”

Section: Osnac2 Is Highly Expressed During Leaf Senescence and Positimentioning

confidence: 99%

A Rice NAC Transcription Factor Promotes Leaf Senescence via ABA Biosynthesis

et al. 2017

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It is well known that abscisic acid (ABA)-induced leaf senescence and premature leaf senescence negatively affect the yield of rice (Oryza sativa). However, the molecular mechanism underlying this relationship, especially the upstream transcriptional network that modulates ABA level during leaf senescence, remains largely unknown. Here, we demonstrate a rice NAC transcription factor, OsNAC2, that participates in ABA-induced leaf senescence. Overexpression of OsNAC2 dramatically accelerated leaf senescence, whereas its knockdown lines showed a delay in leaf senescence. Chromatin immunoprecipitation-quantitative PCR, dual-luciferase, and yeast one-hybrid assays demonstrated that OsNAC2 directly activates expression of chlorophyll degradation genes, OsSGR and OsNYC3. Moreover, ectopic expression of OsNAC2 leads to an increase in ABA levels via directly up-regulating expression of ABA biosynthetic genes (OsNCED3 and OsZEP1) as well as down-regulating the ABA catabolic gene (OsABA8ox1). Interestingly, OsNAC2 is upregulated by a lower level of ABA but downregulated by a higher level of ABA, indicating a feedback repression of OsNAC2 by ABA. Additionally, reduced OsNAC2 expression leads to about 10% increase in the grain yield of RNAi lines. The novel ABA-NAC-SAGs regulatory module might provide a new insight into the molecular action of ABA to enhance leaf senescence and elucidates the transcriptional network of ABA production during leaf senescence in rice.Senescence is the last stage of leaf development. During this period, various changes occur at the physiological, biochemical, and molecular levels. For example, macromolecules including lipids, proteins, and nucleic acids are hydrolyzed, which leads to disassembly of mitochondria and nuclei, and to cell death (Buchanan-Wollaston et al., 2005;Ulker et al., 2007). Although senescence is an active process to salvage nutrients from old tissues, precocious senescence will shorten the growth stage of crops and be unfavorable to agronomic production (Woo et al., 2013).The most distinguishing feature in leaf senescence is the yellowing phenotype, which is a visible marker of the degradation of macromolecules (Kim et al., 2006). The chlorophyll degradation pathway is one of the most characterized ones for macromolecule degradation in plants (Hörtensteiner, 2006). Overexpressing NON-YELLOW COLORING1 (NYC1) or NYC1-like genes in rice (Oryza sativa) can induce degradation of chlorophyll . A pph (encoding pheophytinase) mutant is abnormal in chlorophyll degradation during senescence and therefore exhibits a stay-green phenotype (Schelbert et al., 2009). Mutation of the PAO (Pheophorbide a oxygenase) gene leads to retention of chlorophyll in leaves during dark-induced senescence in Arabidopsis (Arabidopsis thaliana; Pruzinská et al., 2005). Recently, the highly conserved STAY-GREEN (SGR) in higher plants has been identified to be chloroplast-localized dechelatase (Shimoda et al., 2016).The senescence process is highly regulated by a range of important factors. It has been demon...

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The NAC transcription factor ANAC046 is a positive regulator of chlorophyll degradation and senescence in Arabidopsis leaves

Cited by 139 publications

References 61 publications

Spatiotemporal Transcriptome Analysis Provides Insights into Bicolor Tepal Development in Lilium “Tiny Padhye”

Spatiotemporal Transcriptome Analysis Provides Insights into Bicolor Tepal Development in Lilium “Tiny Padhye”

A kiwifruit (Actinidia deliciosa) R2R3‐MYB transcription factor modulates chlorophyll and carotenoid accumulation

A Rice NAC Transcription Factor Promotes Leaf Senescence via ABA Biosynthesis

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