The N-terminus modulates human Caf1 activity, structural stability and aggregation

Feng, Likui; Yan, Yong-Bin

doi:10.1016/j.ijbiomac.2012.05.032

Cited by 2 publications

(3 citation statements)

References 39 publications

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“…When excited at 280 nm, both Trp and Tyr fluorescence can be observed, while only the Trp fluorophore is excited using an excitation wavelength of 295 nm. The emission maximum wavelength was ∼229 nm for both the fluorescence spectra excited at 280 nm or 295 nm, which is similar to that of human Caf1a/CNOT7 [37] or Caf1b/hPop2/CNOT8 [24]. To confirm the result from homology modeling, the Trp fluorescence spectrum obtained by exciting at 295 nm was further analyzed using the theoretical model of discrete states of Trp fluorophores in proteins [29], [30].…”

Section: Resultsmentioning

confidence: 65%

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Biochemical and Biophysical Characterization of the Deadenylase CrCaf1 from Chlamydomonas reinhardtii

Zhang

Yan

2013

PLoS ONE

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The modulation of mRNA turnover has been increasingly recognized as a hotpoint for gene expression regulation at the post-transcriptional level. In eukaryotic cells, most mRNAs are degraded via the deadenylation-dependent pathway, in which the removal of the poly(A) tail is the initial and rate-limiting step. Caf1, a deadenylase specifically degrades poly(A) from the 3′-end, is highly conserved from yeast to mammalians. Caf1s in higher plants have been shown to be involved in plant development and stress response. However, little is known about the biochemical and biophysical properties of Caf1s in plants. In this research, we cloned the crcaf1 gene from Chlamydomonas reinhardtii and studied the properties of the recombinant proteins. The results showed that CrCaf1 was a deadenylase with conserved sequence motifs, structural features, and catalytic properties of the Caf1 family. CrCaf1 degraded poly(A) in a distributive mode with the optimal reacting conditions at pH 7 and 35°C. CrCaf1 had similar activity when coordinated with Mg2+ and Mn2+, while the enzyme bound to Ca2+ or Zn2+ was almost inactivated. Zn2+ could induce CrCaf1 aggregation with the disruption of the native structure, while Mg2+, Mn2+ and Ca2+ could stabilize CrCaf1 against thermal denaturation by reducing protein aggregation. Among the various metal ions, Mn2+ showed the strongest protective effect on CrCaf1 stability, implying that Mn2+ might play a role in regulating CrCaf1 stability in the C. reinhardtii cells under some stressed conditions. These findings provide a starting point for further investigation of the physiological functions of CrCaf1 in C. reinhardtii.

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Section: Resultsmentioning

confidence: 65%

“…As shown in Figure 3A, the elution volume of CrCaf1 in the SEC profile using Superdex 200 10/30 GL column was about 14.9 ml. This indicated that the recombinant CrCaf1 existed as a monomer in solutions, which was similar to Caf1s from the other species [18], [19], [24], [37].…”

Section: Resultsmentioning

confidence: 69%

Biochemical and Biophysical Characterization of the Deadenylase CrCaf1 from Chlamydomonas reinhardtii

Zhang

Yan

2013

PLoS ONE

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“…In the present study, the IbPSS1 gene level in the low salinity group was 3.6 times higher than in the high salinity group. As a common tolerance-related gene in plants, CAF1 family gene belongs to the DEDD family, participates in the regulation of mRNA degradation, and plays a crucial role in stress response [ 27 , 28 ]. In this study, IbCAF1 showed high expression in the low salinity group, about 2.19 times higher than in the high salinity group.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of Physiological Coping Strategies and Quality Substances in Purple SweetPotato under Different Salinity Levels

Wang

Dai

Liu³

et al. 2022

Genes

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Although salinity stress is one of the principal abiotic stresses affecting crop yield, a suitable concentration of NaCl has proven to be useful for increasing crop quality. This study used low salinity (34 mmol/L NaCl) and high salinity (85 mmol/L) to cultivate purple sweetpotato. Using transcriptomics and metabolomics to profile the pathway indicated that glycometabolism, secondary metabolite biosynthesis and the starch catabolic process were the significant pathways under the salinity stress. Further research showed that purple sweetpotato could regulate genes related to the regulation of the cellular Na+, K+, and other ions concentration in response to the low salinity tolerance, but loses this ability under high salinity. Meanwhile, under low salinity, the activity of antioxidant enzymes and their related gene expression are maintained at a high level. The low salinity influences the monosaccharide composition as well as the content and regulation of genes related to starch synthesis. Quality analysis showed that the low salinity could increase the starch content and influence the amylopectin biosynthesis. It suggested that low salinity promotes substance accumulation. High salinity could increase the anthocyanins biosynthesis and low salinity had a significant impact on phenolic acid and flavonol. Finally, the gene expression levels also prove the low salinity could change the composition and content level of the purple sweetpotato. This study showed that an appropriate concentration of NaCl can be used as an elicitor for application in purple sweetpotato planting.

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The N-terminus modulates human Caf1 activity, structural stability and aggregation

Cited by 2 publications

References 39 publications

Biochemical and Biophysical Characterization of the Deadenylase CrCaf1 from Chlamydomonas reinhardtii

Biochemical and Biophysical Characterization of the Deadenylase CrCaf1 from Chlamydomonas reinhardtii

Evaluation of Physiological Coping Strategies and Quality Substances in Purple SweetPotato under Different Salinity Levels

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