The N-Terminal Domain of the Flo1 Flocculation Protein from Saccharomyces cerevisiae Binds Specifically to Mannose Carbohydrates

Goossens, Katty; Stassen, Catherine; Stals, Ingeborg; Donohue, Dagmara; Devreese, Bart; Greve, Henri De; Willaert, Ronnie

doi:10.1128/ec.00185-10

Cited by 40 publications

(47 citation statements)

References 45 publications

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“…Major features are N-terminal PA14 domains that bind a-mannosides and mediate adhesion to adjacent cells, a central Ser/Thr-rich domain that is organized in repeat sequences and heavily glycosylated, and two or three conserved three-Cys repeats toward their C termini (Verstrepen and Klis 2006;Goossens and Willaert 2010;Klis et al 2010;Veelders et al 2010;Goossens et al 2011). In addition, the Ser/Thr-rich domains of Flo1 and Flo11/Muc1 have short sequences enriched in Ile, Thr, and Val that are predicted to form intramolecular b-sheetlike interactions or amyloids, and both a soluble, GPI-less portion of Flo11/Muc1 and a Flo1-derived form fibrillar b-aggregates in vitro (Ramsook et al 2010).…”

Section: Nonenzymatic Cwpsmentioning

confidence: 99%

Architecture and Biosynthesis of the Saccharomyces cerevisiae Cell Wall

2012

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The wall gives a Saccharomyces cerevisiae cell its osmotic integrity; defines cell shape during budding growth, mating, sporulation, and pseudohypha formation; and presents adhesive glycoproteins to other yeast cells. The wall consists of b1,3-and b1,6-glucans, a small amount of chitin, and many different proteins that may bear N-and O-linked glycans and a glycolipid anchor. These components become cross-linked in various ways to form higher-order complexes. Wall composition and degree of cross-linking vary during growth and development and change in response to cell wall stress. This article reviews wall biogenesis in vegetative cells, covering the structure of wall components and how they are cross-linked; the biosynthesis of N-and O-linked glycans, glycosylphosphatidylinositol membrane anchors, b1,3-and b1,6-linked glucans, and chitin; the reactions that cross-link wall components; and the possible functions of enzymatic and nonenzymatic cell wall proteins. TABLE OF CONTENTS Abstract 775Introduction 777

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Section: Nonenzymatic Cwpsmentioning

confidence: 99%

Architecture and Biosynthesis of the Saccharomyces cerevisiae Cell Wall

2012

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“…Flocculins are lectin‐like cell wall proteins that bind to mannose residues on the cell wall of neighboring cells leading to flocculation (Van Mulders et al , , Goossens et al ., ). Yeast subtelomeric loci carry five dominant FLO genes; FLO1 , FLO5 , FLO9 , FLO10 and FLO11 , all of which are epigenetically regulated (Halme et al , ) and responsible for flocculation and biofilm formation (Smukalla et al , ; Goossens et al , ; Sim et al , ). FLO1 and FLO11 are highly variable in terms of expression and sequence among different strains of Saccharomyces cerevisiae (Zara et al , ).…”

Section: Introductionmentioning

confidence: 99%

Flocculation of Saccharomyces cerevisiae is dependent on activation of Slt2 and Rlm1 regulated by the cell wall integrity pathway

Sariki

Kumawat

Singh

et al. 2019

Molecular Microbiology

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Summary Flocculation is an essential characteristic of yeast cells required for survival under adverse conditions. The multicellular structure (flocs) of yeast provides a suitable microenvironment to enhance the chances of survival during stress conditions. Although the signaling events triggering flocculation have been studied earlier, molecular mechanisms remain elusive. In the present study, we used flocculating sen1 mutants to identify the mechanism of flocculation. Based on the abnormal cell surface morphology and constitutive phosphorylation of Slt2p in flocculating sen1 mutant cells, we hypothesized if flocculation was regulated by the cell wall integrity (CWI) pathway. Up‐regulation of FLO genes in wild‐type cells was observed upon the activation of CWI pathway either by chemical treatment or by deleting Slt2 phosphatase (Msg5). Our study with Slt2 mutants reveals that the active state of Slt2 is indispensable for flocculation. Deletion of either SLT2 or RLM1 leads to reduced flocculation. Furthermore, we observed overlapping binding sites for Rlm1 and Tup1 at the promoters of almost all the FLO genes. Finally, we show higher Rlm1 and lower Tup1 occupancy at the promoters of FLO1 and FLO5 in flocculating cells. Altogether we demonstrate that CWI MAPK (Slt2) pathway uses a non‐catalytic mechanism to activate the transcription of FLO genes.

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“…Cell-to-cell adhesion occurs via binding between the flocculin and surface carbohydrates in a calcium-dependent manner [5]. The bound carbohydrates consist of various sugars including mannose, glucose, and galactose that are specific to the type of flocculin and yeast species [6]–[8]. There has been considerable interest in elucidating the genetic control of flocculation to better understand this phenomenon and generate biotechnological advances in yeast-based industries.…”

Section: Introductionmentioning

confidence: 99%

Deciphering the Transcriptional-Regulatory Network of Flocculation in Schizosaccharomyces pombe

et al. 2012

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In the fission yeast Schizosaccharomyces pombe, the transcriptional-regulatory network that governs flocculation remains poorly understood. Here, we systematically screened an array of transcription factor deletion and overexpression strains for flocculation and performed microarray expression profiling and ChIP–chip analysis to identify the flocculin target genes. We identified five transcription factors that displayed novel roles in the activation or inhibition of flocculation (Rfl1, Adn2, Adn3, Sre2, and Yox1), in addition to the previously-known Mbx2, Cbf11, and Cbf12 regulators. Overexpression of mbx2+ and deletion of rfl1+ resulted in strong flocculation and transcriptional upregulation of gsf2 +/pfl1+ and several other putative flocculin genes (pfl2+–pfl9+). Overexpression of the pfl+ genes singly was sufficient to trigger flocculation, and enhanced flocculation was observed in several combinations of double pfl+ overexpression. Among the pfl1+ genes, only loss of gsf2+ abrogated the flocculent phenotype of all the transcription factor mutants and prevented flocculation when cells were grown in inducing medium containing glycerol and ethanol as the carbon source, thereby indicating that Gsf2 is the dominant flocculin. In contrast, the mild flocculation of adn2+ or adn3+ overexpression was likely mediated by the transcriptional activation of cell wall–remodeling genes including gas2+, psu1+, and SPAC4H3.03c. We also discovered that Mbx2 and Cbf12 displayed transcriptional autoregulation, and Rfl1 repressed gsf2+ expression in an inhibitory feed-forward loop involving mbx2+. These results reveal that flocculation in S. pombe is regulated by a complex network of multiple transcription factors and target genes encoding flocculins and cell wall–remodeling enzymes. Moreover, comparisons between the flocculation transcriptional-regulatory networks of Saccharomyces cerevisiae and S. pombe indicate substantial rewiring of transcription factors and cis-regulatory sequences.

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The N-Terminal Domain of the Flo1 Flocculation Protein from Saccharomyces cerevisiae Binds Specifically to Mannose Carbohydrates

Cited by 40 publications

References 45 publications

Architecture and Biosynthesis of the Saccharomyces cerevisiae Cell Wall

Architecture and Biosynthesis of the Saccharomyces cerevisiae Cell Wall

Flocculation of Saccharomyces cerevisiae is dependent on activation of Slt2 and Rlm1 regulated by the cell wall integrity pathway

Deciphering the Transcriptional-Regulatory Network of Flocculation in Schizosaccharomyces pombe

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