The N-butyl-N-4-hydroxybutyl Nitrosamine Mouse Urinary Bladder Cancer Model

Oliveira, Paula A.; Nóbrega, Cármen; Costa, Rui M. Gil da; Arantes‐Rodrigues, Regina

doi:10.1007/978-1-4939-7234-0_13

Cited by 15 publications

(11 citation statements)

References 64 publications

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“…The most established mechanism involves the formation of endogenous nitrosamines from nitrites that are particularly abundant in processed meats (17). Experimental studies have confirmed that some nitrosamine metabolites induced bladder tumors in animal models (18). In addition, tobacco smoke, a well-established risk factor for bladder cancer, is a main source of exogenous exposure to nitrosamines.…”

Section: Discussionmentioning

confidence: 99%

Processed Meat Intake and Bladder Cancer Risk in the Prostate, Lung, Colorectal, and Ovarian (PLCO) Cohort

2019

Cancer Epidemiology, Biomarkers &Amp; Prevention

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Background: The association between processed meat intake and bladder cancer risk has been evaluated by several observational studies with inconsistent results.Methods: In a cohort of 101,721 subjects in the Prostate, Lung, Colorectal, and Ovarian (PLCO) Cancer Screening Trial, we analyzed the association of processed meat intake with bladder cancer risk.Results: After a median of 12.5 years of follow-up, 776 new cases of bladder cancer were identified. Intake of processed red meat was significantly associated with the incidence of bladder cancer after multivariate adjustment [highest vs. lowest quintile: HR, 1.47; 95% confidence interval (CI), 1.12-1.93; P trend ¼ 0.008]. In contrast, there was only a suggestive but not significant association between intake of total processed meat and bladder cancer risk after multivariable adjustment (highest vs. lowest quintile: HR, 1.16; 95% CI, 0.89-1.50; P trend ¼ 0.073).Conclusions: This large prospective study suggests that intake of processed red meat is associated with a higher risk of bladder cancer.Impact: Bladder cancer risk is increased with cumulative intake of processed red meat.

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Section: Discussionmentioning

confidence: 99%

Processed Meat Intake and Bladder Cancer Risk in the Prostate, Lung, Colorectal, and Ovarian (PLCO) Cohort

2019

Cancer Epidemiology, Biomarkers &Amp; Prevention

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“…To test our hypothesis that mice with bladder cancer would harbor a distinct urinary "oncobiome", we used an established model in which a dilute bladder-specific carcinogen (n-butyl-n-(4-hydroxybutyl) nitrosamine, BBN) is administered to mice in their drinking water. The BBN model, a widely used and well-established model of bladder carcinogenesis, [22,23] reliably leads to tumor formation in both mice and rats. Although the incidence of cancer is not 100%, this can be seen as an advantage given that an array of pathologies are obtained.…”

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confidence: 99%

Profiling of urine bacterial DNA to identify an “oncobiome” in a mouse model of bladder cancer

Banskota

et al. 2018

Preprint

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Introduction: Recurrent urinary tract infections have been linked to increased risk of bladder cancer, suggesting a potential role of the urinary microbiome in bladder cancer pathogenesis.Objective: Compare the urinary microbiomes in mice with and without bladder. Methods:Longitudinal study of mice exposed to a dilute bladder-specific carcinogen (0.05% nbutyl-n-(4-hydroxybutyl) nitrosamine, BBN mice, n=10), and control mice (n=10). Urine was sampled monthly from individual mice for 4 months. Microbial DNA was extracted from the urine, and the V4 region of the 16S rRNA gene sequenced. Animals were sacrificed and their bladders harvested for histopathology. Bladder sections were graded by a blinded pathologist. The composition and diversity of the urinary microbiome were compared between the BBN and control mice. Metabolic pathway analysis was completed using PICRUST.Results: Bladder histology in the BBN group showed normal tissue with inflammation (BBNnormal, n=5), precancerous pathologies, (BBN-precancerous, n=3), and invasive cancer (BBNcancer, n=2). Alpha diversity did not differ between the mice exposed to BBN and the control mice at any timepoint. There were no differences in the urinary microbiomes between the BBN and control mice at baseline. At month 4, mice exposed to BBN had higher proportion of both Gardnerella and Bifidobacterium compared to control mice. There were no differences in proportions of specific bacteria between either the BBN-precancer or BBN-cancer and controls at month 4. However, the BBN-normal mice had higher proportions of Gardnerella, Haemophilus, Bifidobacterium, and Ureaplasma Actinobaculum, and lower proportions of Actinomyces, compared to control mice at month 4. Functional pathway analysis demonstrated increases in genes related to purine metabolism, phosphotransferase systems, peptidases, protein folding, and bacterial toxins in the BBN-mice compared to control mice at month 4.Conclusion: Mice exposed to 4 months of BBN, a bladder-specific carcinogen, have distinct urine microbial profiles compared to control mice.

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“…Bladder, as an organ of the urinary system in the body, is a necessary path for urine excretion. When bladder lesions occur, such as nearby ureteral obstruction, internal cancer and other conditions that make the urinary system unable to work properly, the construction of the model is indispensable (Obajemu et al, 2018, Xiao et al, 2017, Sole et al, 2019, Oliveira et al, 2018). In urinary clinical diagnosis, patients are often required to insert the catheter for catheterization, so modeling is one of the methods to accelerate the understanding of its principle.…”

Section: Discussionmentioning

confidence: 99%

Construction and application of urinary system model with functional bladder module

Liu

2019

Saudi Journal of Biological Sciences

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In order to study the construction and application of urinary system model with functional bladder module, bladder model was designed, and appropriate materials was selected to make it, and its performance was studied. The results showed that in the analysis of pressure performance of bladder model, more detrusor instability was found in the model than in the urodynamic test, and there was significant statistical difference (P < 0.01). In the analysis of bladder safety capacity, it was found that the bladder safety capacity in the model was much larger than that measured by urodynamics, and there was significant statistical difference (P < 0.01). In the analysis of detrusor workmanship and contraction rate, it was found that the normal model group was significantly smaller than the obstruction group, and there was significant statistical difference (P < 0.01). Comparing the detrusor contraction rate of the two groups, it was found that the normal group and the obstruction group had significant difference at t3, and there was no statistical difference between the other two groups. Therefore, through this study, it is found that the understanding of urinary system can be enhanced by building bladder model, and the basic operating skills of medical staff can be improved more easily by using bladder model, which achieves the expected results of the experiment. Although some shortcomings have been found in the course of the study, it still provides experimental reference for the clinical study of bladder in the future.

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The N-butyl-N-4-hydroxybutyl Nitrosamine Mouse Urinary Bladder Cancer Model

Cited by 15 publications

References 64 publications

Processed Meat Intake and Bladder Cancer Risk in the Prostate, Lung, Colorectal, and Ovarian (PLCO) Cohort

Processed Meat Intake and Bladder Cancer Risk in the Prostate, Lung, Colorectal, and Ovarian (PLCO) Cohort

Profiling of urine bacterial DNA to identify an “oncobiome” in a mouse model of bladder cancer

Construction and application of urinary system model with functional bladder module

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