The Myxococcus xanthus rfbABC operon encodes an ATP-binding cassette transporter homolog required for O-antigen biosynthesis and multicellular development

Guo, Dongchuan; Bowden, M. Gabriela; Pershad, Rashmi; Kaplan, Heidi B.

doi:10.1128/jb.178.6.1631-1639.1996

Cited by 61 publications

(65 citation statements)

References 46 publications

(76 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The M. xanthus sasA locus, required for lipopolysaccharide O-antigen biosynthesis, contains rfbA, rfbB, and rfbC (16,17). The rfbA and rfbB genes encode an ABC transporter (17). Furthermore, the fdgA and the sasA mutants show similar phenotypes in that fruiting body formation is impaired and sporulation efficiency is reduced during development (16).…”

Section: Discussionmentioning

confidence: 78%

“…The M. xanthus sasA locus, required for lipopolysaccharide O-antigen biosynthesis, contains rfbA, rfbB, and rfbC (16,17). The rfbA and rfbB genes encode an ABC transporter (17).…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, the deletion of fdgA resulted in defective fruiting body formation and reduced sporulation efficiency. In addition, the induction of the sasA locus that is required for the biosynthesis of the lipopolysaccharide O-antigen and development (16,17) was not detected in the ⌬fruA strain during development.…”

Section: Fruiting Body Development In Myxococcus Xanthus Is a Multicementioning

confidence: 99%

“…Expression of the sasA Locus-Genes at the sasA locus that are involved in lipopolysaccharide O-antigen biosynthesis have been shown to be essential for development (see "Discussion" for details) (16,17). Therefore, we next examined whether the sasA locus is regulated by FruA.…”

Section: Identification Of a Gene Regulated Bymentioning

confidence: 99%

See 3 more Smart Citations

Identification of a Gene Involved in Polysaccharide Export as a Transcription Target of FruA, an Essential Factor for Myxococcus xanthus Development

Ueki

Inouye

2005

Journal of Biological Chemistry

View full text Add to dashboard Cite

Fruiting body development in Myxococcus xanthus is a multicellular event that is coordinated by exchanging intercellular signals.FruA is a transcription factor essential for fruiting body development and is thought to play a key role in the C-signal pathway. Here we present the first identification of a gene regulated by FruA. The gene was isolated from a genomic library via in vitro selection in a DNA binding assay by using the DNA-binding domain of FruA tagged with His 8 at the C-terminal end (FruA-DBD-H 8 ). The gene, named fdgA (FruA-dependent gene A), encodes a protein homologous to the outer-membrane auxiliary family protein involved in the polysaccharide export system. FruA-DBD-H 8 bound the upstream promoter region of the fdgA gene from nucleotide ؊89 to nucleotide ؊64 with respect to the transcription initiation site, which was required for the induction of fdgA expression during development. fdgA mRNA induced during development was absent in a fruA deletion strain. The deletion of fdgA resulted in defective fruiting body formation and reduced sporulation efficiency (1% that of the parent strain). Moreover, FruA was required for the developmental expression of sasA, which is also involved in the biosynthesis of the lipopolysaccharide O-antigen and is required for fruiting body development. Furthermore, the expression of both fdgA and sasA was partially dependent on the C-signal. These findings expand our understanding of the signal transduction pathway mediated by FruA during development in M. xanthus.

show abstract

Section: Discussionmentioning

confidence: 78%

“…The M. xanthus sasA locus, required for lipopolysaccharide O-antigen biosynthesis, contains rfbA, rfbB, and rfbC (16,17). The rfbA and rfbB genes encode an ABC transporter (17).…”

Section: Discussionmentioning

confidence: 99%

Section: Fruiting Body Development In Myxococcus Xanthus Is a Multicementioning

confidence: 99%

Section: Identification Of a Gene Regulated Bymentioning

confidence: 99%

See 2 more Smart Citations

Identification of a Gene Involved in Polysaccharide Export as a Transcription Target of FruA, an Essential Factor for Myxococcus xanthus Development

Ueki

Inouye

2005

Journal of Biological Chemistry

View full text Add to dashboard Cite

show abstract

“…The double mutants YZ144 (⌬difC S Ϫ ) and YZ145 (⌬difC A Ϫ ) were constructed by Mx4-mediated transduction using SW403 as the recipient strain and DK3473 and MXH1216 as donor strains, respectively. pKB106, the plasmid used for ectopic complementation, was constructed by cloning a 2.76-kb PstI-BamHI fragment containing the difC gene into pYC274, an M. xanthus integrative vector with the Mx8 attachment site (15). This PstI-BamHI fragment contains sequences immediately downstream of difA to the 5Ј portion of difE.…”

Section: Methodsmentioning

confidence: 99%

A CheW Homologue Is Required for Myxococcus xanthus Fruiting Body Development, Social Gliding Motility, and Fibril Biogenesis

Bellenger

Shi

et al. 2002

J Bacteriol

View full text Add to dashboard Cite

In bacteria with multiple sets of chemotaxis genes, the deletion of homologous genes or even different genes in the same operon can result in disparate phenotypes. Myxococcus xanthus is a bacterium with multiple sets of chemotaxis genes and/or homologues. It was shown previously that difA and difE, encoding homologues of the methyl-accepting chemoreceptor protein (MCP) and the CheA kinase, respectively, are required for M. xanthus social gliding (S) motility and development. Both difA and difE mutants were also defective in the biogenesis of the cell surface appendages known as extracellular matrix fibrils. In this study, we investigated the roles of the CheW homologue encoded by difC, a gene at the same locus as difA and difE. We showed that difC mutations resulted in defects in M. xanthus developmental aggregation, sporulation, and S motility. We demonstrated that difC is indispensable for wild-type cellular cohesion and fibril biogenesis but not for pilus production. We further illustrated the ectopic complementation of a difC in-frame deletion by a wild-type difC. The identical phenotypes of difA, difC, and difE mutants are consistent and supportive of the hypothesis that the Dif chemotaxis homologues constitute a chemotaxis-like signal transduction pathway that regulates M. xanthus fibril biogenesis and S motility.

show abstract

Carbohydrate‐Active Enzymes Database: Principles and Classification of Glycosyltransferases

Coutinho

Rancurel

Stam

et al. 2009

Bioinformatics for Glycobiology and Glycomics

View full text Add to dashboard Cite

The Myxococcus xanthus rfbABC operon encodes an ATP-binding cassette transporter homolog required for O-antigen biosynthesis and multicellular development

Cited by 61 publications

References 46 publications

Identification of a Gene Involved in Polysaccharide Export as a Transcription Target of FruA, an Essential Factor for Myxococcus xanthus Development

Identification of a Gene Involved in Polysaccharide Export as a Transcription Target of FruA, an Essential Factor for Myxococcus xanthus Development

A CheW Homologue Is Required for Myxococcus xanthus Fruiting Body Development, Social Gliding Motility, and Fibril Biogenesis

Carbohydrate‐Active Enzymes Database: Principles and Classification of Glycosyltransferases

Contact Info

Product

Resources

About