The myeloic related protein MRP8/14 (27E10 antigen)— usefulness as a potential marker for disease activity in ulcerative colitis and putative biological function

Abstract

Abstract. MRP8, MRP14 and their heterodimer MRP8/14 (27E10 antigen) are myeloic related proteins which have been shown to have a major role in inflammatory and immunological responses. In the present study monospecific antibodies against MRPs were used to investigate immunohistochemically the distribution of these proteins in routinely processed bowel tissues from 23 patients with ulcerative colitis (UC). MRP8, MRP14 and their heterocomplex MRP8/l4 were demonstrated in the majority of granulocytes and macrophages in tissues of patients with active UC. Furthermore by employing the ELISA technique we measured MRP8/14 serum levels in 62 patients with UC and the results were compared with those for healthy controls. Disease activities were determined by established clinical activity indices. Serum MRP8/14 concentrations were significantly ( P < 0.0001) increased in patients with active ulcerative colitis. No enhancement of serum levels were found for MRP14 and MRP8 alone, respectively. The follow-up of individual patients with initially active disease showed a decrease of MRP8/14 serum levels in parallel with clinical improvement following the start of therapy. It is thus concluded that MRP8/14 accurately reflects the degree of disease activity in UC. Further, possible biological function of MRPs seems to be associated with the heterodimeric form (27E10 antigen) rather than with individual proteins. Our morphological results confirm the finding of enhanced MRP8/14 serum levels in patients with active UC.

Increased Levels of Calprotectin in Obesity Are Related to Macrophage Content: Impact on Inflammation and Effect of Weight Loss

“…A hallmark of S100/calgranulin polypeptides is their association with chronic inflammation, such as human inflammatory bowel diseases (43). A positive association of calprotectin with CRP, IL-6, PAI-1 and TNF-α, well-established markers of chronic inflammation, was observed in the present study.…”

RAGE and arthritis: the G82S polymorphism amplifies the inflammatory response

“…For example, in addition to their association with disease severity in RA, 4 S100/calgranulins have been linked to disease progression in inflammatory bowel disease. 36,37 Furthermore, strongly increased expression of a member of this family of molecules, psoriasin, has been demonstrated in psoriatic lesions compared with adjacent unaffected skin in human subjects, 38 thus further supporting the premise that S100/calgranulins may not simply be 'innocent bystanders', but, rather, by virtue of their engagement of RAGE, 1 active participants in the host inflammatory response. We speculate that triggered by interaction with accumulating S100/calgranulins, activation of RAGE engages key signalling pathways linked to proinflammatory responses, such as MAP kinases and NF-B, thereby amplifying expression of molecules linked to chronic cellular perturbation and tissue destruction.…”

Morphological characteristics of coronary atherosclerosis in diabetes mellitus

“…An association between soluble S100 proteins and human inflammatory bowel disease has been demonstrated (20). The interactions between S100 Ca 2+ -modulated proteins and RAGE receptors in the fine regulation of leukocyte trafficking and proliferation has been reviewed (21), but the role of EN-RAGE in atherosclerosis-related inflammation has not been studied.…”