The Mycobacterium tuberculosis membrane protein Rv0180c: Evaluation of peptide sequences implicated in mycobacterial invasion of two human cell lines

Caceres, Silvia M.; Ocampo, Marisol; Arévalo-Pinzón, Gabriela; Jimenez, Ronald; Patarroyo, Manuel Elkín; Patarroyo, Manuel A.

doi:10.1016/j.peptides.2010.09.017

Cited by 17 publications

(12 citation statements)

References 74 publications

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“…Also detected only in MIRU cluster B was a premature stop mutation in Rv0180c at the third amino acid of the coding sequence—effectively eliminating transcription of this molecule. This gene encodes a previously described probable transmembrane protein that is involved in interaction with and invasion of monocytes and alveolar epithelium in the members of the MTB complex [ 59 ]. Our finding of SNVs associated with abrogated production of Rv0180c, demonstrate that in these clinical strains of MTB, infection has occurred in the absence of this molecule.…”

Section: Discussionmentioning

confidence: 99%

Application of whole genome sequence analysis to the study of Mycobacterium tuberculosis in Nunavut, Canada

et al. 2017

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Canada has one of the lowest rates of tuberculosis (TB) in the world, however, among certain sub-populations, disease incidence rates approach those observed in sub-Saharan Africa, and other high incidence regions. In this study, we applied mycobacterial interspersed repetitive unit (MIRU) variable number of tandem repeat (VNTR) and whole genome sequencing (WGS) to the analysis of Mycobacterium tuberculosis isolates obtained from Northern communities in the territory of Nunavut. WGS was carried out using the Illumina MiSeq, with identified variants used to infer phylogenetic relationships and annotated to infer functional implications. Additionally, the sequencing data from these isolates were augmented with publically available WGS to evaluate data from the Nunavut outbreak in the broader Canadian context. In this study, isolates could be classified into four major clusters by MIRU-VNTR analysis. These could be further resolved into sub-clusters using WGS. No evidence for antimicrobial resistance, either genetic or phenotypic, was observed in this cohort. Among most subjects with multiple samples, reactivation/incomplete treatment likely contributed to recurrence. However, isolates from two subjects appeared more likely to have occurred via reinfection, based on the large number of genomic single nucleotide variants detected. Finally, although quite distinct from previously reported Canadian MTB strains, isolates obtained from Nunavut clustered most closely with a cohort of samples originating in the Nunavik region of Northern Quebec. This study demonstrates the benefit of using WGS for discriminatory analysis of MTB in Canada, especially in high incidence regions. It further emphasizes the importance of focusing epidemiological intervention efforts on interrupting transmission chains of endemic TB throughout Northern communities, rather than relying on strategies applied in regions where the majority of TB cases result from importation of foreign strains.

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Section: Discussionmentioning

confidence: 99%

Application of whole genome sequence analysis to the study of Mycobacterium tuberculosis in Nunavut, Canada

et al. 2017

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“…Two novel proteins Rv0679c and Rv0180c were identified from M.tb H37Rv, which are membrane bound and play a role in entry / invasion of pathogen into host cells [4,5]. Rv0679c protein was classified as a membrane protein of the envelope of M.tb with a molecular mass of 16.5 KDa, consisting of 165 amino acids and contains a putative N-terminal signal sequence and a consensus lipoprotein-processing motif.…”

Section: Introductionmentioning

confidence: 99%

“…Cifuentes et al [4] have shown that peptides of Rv0679c inhibited invasion / enrty of live M.tb bacilli into A549 and U937 macrophage cell lines in vitro. Rv0180c is a conserved transmembrane protein with a molecular mass of 47.5 KDa, consisting of 452 amino acids with unknown function however shares a high sequence similarity with putative conserved membrane and transmembrane proteins from different mycobacterial species [5]. Caceres [5] have shown that the peptides of this protein inhibited the invasion of M.tb H37Rv into U937 and A549 cell lines in vitro indicating its candidature as a subunit based vaccine against tuberculosis.…”

Section: Introductionmentioning

confidence: 99%

“…Rv0180c is a conserved transmembrane protein with a molecular mass of 47.5 KDa, consisting of 452 amino acids with unknown function however shares a high sequence similarity with putative conserved membrane and transmembrane proteins from different mycobacterial species [5]. Caceres [5] have shown that the peptides of this protein inhibited the invasion of M.tb H37Rv into U937 and A549 cell lines in vitro indicating its candidature as a subunit based vaccine against tuberculosis. Either Rv0679c or Rv0180c protein were not evaluated for the possible mechanism of blocking invasion into the cells, which could be probably through specific receptors.…”

Section: Introductionmentioning

confidence: 99%

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Computational Analysis of Interaction between Mycobacterial Antigens Rv0679c and Rv0180c with Toll like Receptors of Human and Mouse

Lavarti¹,

Ganugapati²,

Ratcha³

et al. 2016

IJCA

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Toll like receptors (TLRs) play a key role in the innate immune response to infectious agents. The Mycobacterium tuberculosis bacilli and its antigens interaction with TLR-2-1 and TLR-4-MD2 were shown to activate intracellular signalling which determines the outcome of the disease. Present study focused on two new antigens Rv0679c and Rv0180c of M.tb H37Rv and their interactions with human and mouse TLRs using computational approach. Structures of Rv0679c and Rv0180c antigens of M.tb were generated using I-TASSER and docked with TLR-2-1 and TLR-4-MD2 complexes of human and mouse using Cluspro2.0. Rv0180c antigen has better binding energy to both human and mouse TLR-2-1 and TLR-4-MD2 compared to Rv0679c and other mycobacterial antigens. The Rv0679c and Rv0180c antigens have better binding energy to TLR 4-MD2 complex compared to TLR-2-1 in both human and mouse. Both antigens has better binding energy to mouse TLRs compared to human except where Rv0180c has better binding to human TLR-2-1. Our findings suggest that Rv0180c might be the preferred for binding to TLRs than Rv0679c and within TLRs, TLR-4-MD2 for interaction. General TermsInvasive mechanism of mycobacterial antigens using TLRs

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“…Mce family proteins) [16]. Experimental results obtained in our laboratory have led to some Mtb proteins' involvement in such invasion having been described, assessed and characterized in terms of their specific binding ability and role in mycobacterial entry to A549 cells and U937 monocyte-derived macrophages [5,18,40,42,50,59]. Identifying surface proteins (or their specific segments) in intracellular pathogens such as Plasmodium falciparum has been shown to counteract invasion, thereby becoming an important approach in the search for minimal subunit-based, multi-epitope, synthetic vaccine candidates [43].…”

Section: Introductionmentioning

confidence: 99%

Mycobacterium tuberculosis surface protein Rv0227c contains high activity binding peptides which inhibit cell invasion

et al. 2012

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The Mycobacterium tuberculosis membrane protein Rv0180c: Evaluation of peptide sequences implicated in mycobacterial invasion of two human cell lines

Cited by 17 publications

References 74 publications

Application of whole genome sequence analysis to the study of Mycobacterium tuberculosis in Nunavut, Canada

Application of whole genome sequence analysis to the study of Mycobacterium tuberculosis in Nunavut, Canada

Computational Analysis of Interaction between Mycobacterial Antigens Rv0679c and Rv0180c with Toll like Receptors of Human and Mouse

Mycobacterium tuberculosis surface protein Rv0227c contains high activity binding peptides which inhibit cell invasion

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