The myb-related gene stonewall induces both hyperplasia and cell death in Drosophila: rescue of fly lethality by coexpression of apoptosis inducers

Brun, Sylvain; Rincheval-Arnold, Aurore; Colin, Jessie; Risler, Yanick; Mignotte, Bernard; Guénal, Isabelle

doi:10.1038/sj.cdd.4401861

Cited by 10 publications

(14 citation statements)

References 38 publications

(36 reference statements)

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“…Moreover, we found several proteins involved in the apoptotic pathway, as the transcription factor Stwl 23 , the mitochondrial carrier homolog 1 (Mtch) 24 , ALG-2 interacting protein X 21 and the GTPase Rab1 25 , 26 . Among these proteins, we focused our attention on the ALG-2 interacting protein X, also called Alix.…”

Section: Resultsmentioning

confidence: 99%

The multifunctional polydnavirus TnBVANK1 protein: impact on host apoptotic pathway

Salvia

Grossi

Amoresano

et al. 2017

Sci Rep

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Toxoneuron nigriceps (Hymenoptera, Braconidae) is an endophagous parasitoid of the larval stages of the tobacco budworm, Heliothis virescens (Lepidoptera, Noctuidae). The bracovirus associated with this wasp (TnBV) is currently being studied. Several genes expressed in parasitised host larvae have been isolated and their possible roles partly elucidated. TnBVank1 encodes an ankyrin motif protein similar to insect and mammalian IκB, an inhibitor of the transcription nuclear factor κB (NF-κB). Here we show that, when TnBVank1 was stably expressed in polyclonal Drosophila S2 cells, apoptosis is induced. Furthermore, we observed the same effects in haemocytes of H. virescens larvae, after TnBVank1 in vivo transient transfection, and in haemocytes of parasitised larvae. Coimmunoprecipitation experiments showed that TnBVANK1 binds to ALG-2 interacting protein X (Alix/AIP1), an interactor of apoptosis-linked gene protein 2 (ALG-2). Using double-immunofluorescence labeling, we observed the potential colocalization of TnBVANK1 and Alix proteins in the cytoplasm of polyclonal S2 cells. When Alix was silenced by RNA interference, TnBVANK1 was no longer able to cause apoptosis in both S2 cells and H. virescens haemocytes. Collectively, these results indicate that TnBVANK1 induces apoptosis by interacting with Alix, suggesting a role of TnBVANK1 in the suppression of host immune response observed after parasitisation by T. nigriceps.

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Section: Resultsmentioning

confidence: 99%

The multifunctional polydnavirus TnBVANK1 protein: impact on host apoptotic pathway

Salvia

Grossi

Amoresano

et al. 2017

Sci Rep

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“…All three of these pathways converge on Bim, a pro-apoptotic protein that is activated in response to NGF deprivation. Overexpression of either FOXO or Myb family proteins (including STWL) can result in apoptotic cell death (Liu and Greene 2001;Gilley et al 2003;Brun et al 2006). However, the apoptotic response to NGF under physiological conditions may require the combined derepression of all three of these pathways (Biswas et al 2007).…”

Section: Discussionmentioning

confidence: 99%

A Drosophila Gain-of-Function Screen for Candidate Genes Involved in Steroid-Dependent Neuroendocrine Cell Remodeling

Zhao

Rice

et al. 2008

Genetics

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The normal functioning of neuroendocrine systems requires that many neuropeptidergic cells change, to alter transmitter identity and concentration, electrical properties, and cellular morphology in response to hormonal cues. During insect metamorphosis, a pulse of circulating steroids, ecdysteroids, governs the dramatic remodeling of larval neurons to serve adult-specific functions. To identify molecular mechanisms underlying metamorphic remodeling, we conducted a neuropeptidergic cell-targeted, gain-of-function genetic screen. We screened 6097 lines. Each line permitted Gal4-regulated transcription of flanking genes. A total of 58 lines, representing 51 loci, showed defects in neuropeptide-mediated developmental transitions (ecdysis or wing expansion) when crossed to the panneuropeptidergic Gal4 driver, 386Y-Gal4. In a secondary screen, we found 29 loci that produced wing expansion defects when crossed to a crustacean cardioactive peptide (CCAP)/bursicon neuron-specific Gal4 driver. At least 14 loci disrupted the formation or maintenance of adult-specific CCAP/bursicon cell projections during metamorphosis. These include components of the insulin and epidermal growth factor signaling pathways, an ecdysteroid-response gene, cabut, and an ubiquitin-specific protease gene, fat facets, with known functions in neuronal development. Several additional genes, including three micro-RNA loci and two factors related to signaling by Myb-like proto-oncogenes, have not previously been implicated in steroid signaling or neuronal remodeling.

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“…Research on the egg chamber contributes significantly to scientific knowledge. The egg chamber follicle cell epithelium has been a well-established model system to study cell cycle regulation, cell differentiation 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 , cell polarity 17 18 19 20 , endocytosis 21 22 23 , exocytosis 24 , morphogenesis 19 25 26 27 28 , and cancer metastasis 29 30 . Germline-soma interactions provide developmental cues, especially for anterior-posterior and dorsal-ventral patterning 31 32 33 34 35 36 .…”

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confidence: 99%

Automatic stage identification of Drosophila egg chamber based on DAPI images

Jia

Xie

et al. 2016

Sci Rep

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The Drosophila egg chamber, whose development is divided into 14 stages, is a well-established model for developmental biology. However, visual stage determination can be a tedious, subjective and time-consuming task prone to errors. Our study presents an objective, reliable and repeatable automated method for quantifying cell features and classifying egg chamber stages based on DAPI images. The proposed approach is composed of two steps: 1) a feature extraction step and 2) a statistical modeling step. The egg chamber features used are egg chamber size, oocyte size, egg chamber ratio and distribution of follicle cells. Methods for determining the on-site of the polytene stage and centripetal migration are also discussed. The statistical model uses linear and ordinal regression to explore the stage-feature relationships and classify egg chamber stages. Combined with machine learning, our method has great potential to enable discovery of hidden developmental mechanisms.

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The myb-related gene stonewall induces both hyperplasia and cell death in Drosophila: rescue of fly lethality by coexpression of apoptosis inducers

Cited by 10 publications

References 38 publications

The multifunctional polydnavirus TnBVANK1 protein: impact on host apoptotic pathway

The multifunctional polydnavirus TnBVANK1 protein: impact on host apoptotic pathway

A Drosophila Gain-of-Function Screen for Candidate Genes Involved in Steroid-Dependent Neuroendocrine Cell Remodeling

Automatic stage identification of Drosophila egg chamber based on DAPI images

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