The Myb leucine zipper is essential for leukemogenicity of the v-Myb protein

GATA-1 and c-myb crosstalk during red blood cell differentiation through GATA-1 binding sites in the c-myb promoter

“…Additionally, there was no repression by a mutated À1040 GATA-1 site as expected. To further extend these observations, a reporter plasmid pLucbTATA with low basal activity was used (Bartunek et al, 1997). In this vector the composite À1040 GATA-1/Myb binding site was activated by c-Myb by fivefold (Figure 6b).…”

Fine Structure of the Human Translocation Protein 1 (HTP1/TLOC1) Gene

“…MZF1 (myeloid zinc nger protein) and GATA are known to be important in hematopoiesis (15,16). Evi-1 and v-Myb are implicated in the genesis of myelogenous leukemia (17,18). A major cis-element, AP-1, was also observed.…”

bFGF signaling and v-Myb cooperate in sustained growth of primitive erythroid progenitors

“…For anti-phosphotyrosine (4G10; Upstate Biotechnology, Inc., NY, USA) and anti-bFGF (Transduction Laboratories, KY, USA) monoclonal antibodies, membranes were blocked overnight in TBS (25 mM Tris-HCl pH 7.4, 137 mM NaCl, 5 mM KCl, 0.7 mM CaCl 2 , 0.5 mM MgCl 2 , 0.6 mM Na 2 HPO 4 ) containing 3% BSA, 1 mM EDTA, 0.05% Tween-20 at room temperature, washed twice with wash bu er (50 mM Tris-HCl pH 8.0, 0.1 M NaCl, 0.1% Tween-20) and incubated with ®rst antibody in wash bu er (1 h). For polyclonal antibodies against Myb (Bartunek et al, 1997), GATA-1 (Briegel et al, 1996), C/EBPb (Mink et al, 1996), Mim-1 (Ness et al, 1989), and monoclonal anti-SHC and anti-InsRb antibodies (both Transduction Laboratories, KY, USA), membranes were blocked overnight in 5% non-fat milk powder in TBS or PBS containing 0.05% Tween-20 and incubated with antibody in blocking solution. Subsequently, blots were washed ®ve times with wash bu er and incubated with secondary antibody (ECL kit, Amersham) in TBS supplemented with 5% non-fat milk for 45 min at room temperature.…”