The MX2 macromolecular crystallography beamline: a wiggler X-ray source at the LNLS

Abstract

The Brazilian Synchrotron Light Laboratory [Laboratório Nacional de Luz Síncrotron (LNLS), Campinas, SP, Brazil] is the first commissioned synchrotron light source in the southern hemisphere. The first wiggler macromolecular crystallography beamline (MX2) at the LNLS has been recently constructed and brought into operation. Here the technical design, experimental set-up, parameters of the beamline and the first experimental results obtained at MX2 are described. The beamline operates on a 2.0 T hybrid 30-pole wiggler, and its optical layout includes collimating mirror, Si(111) double-crystal monochromator and toroidal bendable mirror. The measured flux density at the sample position at 8.7 eV reaches 4.8 x 10(11) photons s(-1) mm(-2) (100 mA)(-1). The beamline is equipped with a MarResearch Desktop Beamline Goniostat (MarDTB) and 3 x 3 MarMosaic225 CCD detector, and is controlled by a customized version of the Blu-Ice software. A description of the first X-ray diffraction data sets collected at the MX2 LNLS beamline and used for macromolecular crystal structure solution is also provided.

Structural Insights into Functional Overlapping and Differentiation among Myosin V Motors

“…Data Collection and Structure Solution-Native data of MyoVa-CBD and MyoVc-CBD crystals were collected at 100 K on the W01B-MX2 Beamline at the Brazilian Synchrotron Light Laboratory (Campinas, Brazil) (27), and MyoVb-CBD native data were collected at 100 K on the I02 beamline at the Diamond Light Source (Didcot, United Kingdom). The data were processed and merged with XDS (28) using CC 1/2 cutoffs (29).…”

Bacterial killing via a type IV secretion system

“…Recombinant X-Tfi XAC2610 protein (400 mM dissolved in 10 mM Tris-HCl pH 7.0, 20 mM NaCl) was submitted to crystallization trials and crystals were observed after 2 weeks over a reservoir solution containing 100 mM Bis-Tris-HCl pH 5.5, 200 mM (NH 4 ) 2 SO 4 and 25% (w/v) PEG3350. Crystals were transferred to reservoir solution supplemented with 20% (v/v) glycerol and flash frozen at 100 K. X-ray diffraction data were collected using an in-house Rigaku MicroMax-007 HF microfocus rotating Cu anode generator (Institute of Chemistry, University of São Paulo) and the beamline W01B-MX2 of the Brazilian Synchrotron Light Laboratory (LNLS) 46 . The diffraction data were indexed, integrated and scaled using d*TREK 47 X-ray structure determination and refinement.…”

Insights into the structure and function of fungal β‐mannosidases from glycoside hydrolase family 2 based on multiple crystal structures of the Trichoderma harzianum enzyme

“…The X-ray data collection was carried out on beamline MX1 at the Synchrotron Light National Laboratory (LNLS, Campinas, Brazil) [34] and on beamline ID14-2 at ESRF (Grenoble, France). All datasets were integrated by the use of XDS [35] and scaled with AIMLESS [36].…”