The Mutation of Hb Turriff [α99(G6)Lys→Glu (<i>A</i>AG→<i>G</i>AG)] Is Carried by the α1‐Globin Gene in a Japanese (Hb Turriff‐I)

Alpha hemoglobin stabilizing protein (AHSP) reversibly binds nascent ␣ globin to maintain its native structure and facilitate its incorporation into hemoglobin A. Previous studies indicate that some naturally occurring human ␣ globin mutations may destabilize the protein by inhibiting its interactions with AHSP. However, these mutations could also affect hemoglobin A production through AHSP-independent effects, including reduced binding to ␤ globin. We analyzed 6 human ␣ globin variants with altered AHSP contact surfaces. Alpha globin amino acid substitutions H103Y, H103R, F117S, and P119S impaired interactions with both AHSP and ␤ globin. These mutations are destabilizing in biochemical assays and are associated with microcytosis and anemia in humans. By contrast, K99E and K99N ␣ globins bind ␤ globin normally but exhibit attenuated binding to AHSP. These mutations impair protein folding and expression in vitro and appear to be mildly destabilizing in vivo. In Escherichia coli and erythroid cells, ␣ globin K99E stability is rescued on coexpression with AHSP mutants in which binding to the abnormal globin chain is restored. Our results better define the biochemical properties of some ␣ globin variants and support the hypothesis that AHSP promotes ␣ globin chain stability during human erythropoiesis. (Blood. 2009;113:5961-5969)

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The Mutation of Hb Turriff [α99(G6)Lys→Glu (AAG→GAG)] Is Carried by the α1‐Globin Gene in a Japanese (Hb Turriff‐I)

Cited by 3 publications

References 4 publications

Impaired binding of AHSP to α chain variants: Hb Groene Hart illustrates a mechanism leading to unstable hemoglobins with α thalassemic like syndrome

Impaired binding of AHSP to α chain variants: Hb Groene Hart illustrates a mechanism leading to unstable hemoglobins with α thalassemic like syndrome

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Analysis of human α globin gene mutations that impair binding to the α hemoglobin stabilizing protein

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