The mutation <i>nrpb1‐A325V</i> in the largest subunit of <scp>RNA</scp> polymerase <scp>II</scp> suppresses compromised growth of <i>Arabidopsis</i> plants deficient in a function of the general transcription factor <scp>IIF</scp>

Babiychuk, Elena; Hoang, Khai Trinh; Vandepoele, Klaas; Slijke, Eveline Van De; Geelen, Danny; Jaeger, Geert De; Obokata, Junichi; Kushnir, Sergeï

doi:10.1111/tpj.13417

Cited by 5 publications

(3 citation statements)

References 82 publications

(108 reference statements)

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“…TFIIF function has been established from a wealth of experiments mostly performed in human and yeast, on the one hand, and using a limited number of model TATA box-containing promoters, on the other hand (Luse, 2012). The role of TFIIF, which is still incompletely understood and seem to differ in yeast and mammals, was nearly completely uninvestigated in plants until recently (Babiychuk et al, 2016). In order to describe the role of TFIIFα in Arabidopsis, we characterized different TFIIFα T-DNA insertion mutants.…”

Section: Resultsmentioning

confidence: 99%

“…Here, we showed by yeast two hybrid assay and BiFC in N. benthamiana leaves that the Arabidopsis Topo VI complex is associated with the general transcription factor TFIIF, via the interaction of its BIN4 subunit with the alpha subunit of TFIIF. Recent experiments on the tfIIfβ1 mutant revealed plant growth inhibition and development defects in meristematic organization responsible for stem fasciation and inflorescence impairments (Babiychuk et al, 2016). We also observed growth inhibition in tfIIfα mutants but no such developmental perturbations.…”

Section: Discussionmentioning

confidence: 99%

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TFIIFα interacts with the Topoisomerase VI complex and selectively controls the expression of genes encoding PPR proteins involved in organellar RNA editing in Arabidopsis

Dimnet¹,

Vriet²,

Achard³

et al. 2022

Preprint

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Communication between organelles and the nucleus is referred to as anterograde (nucleus to organelle) and retrograde (organelle to nucleus) signalling. In plants, the pentatricopeptide repeat (PPR) proteins represent a large family of nuclear-encoded proteins that are required for post-transcriptional control of chloroplast and mitochondria gene expression, and hence play a central role in the nuclear anterograde control of organelle genome expression. How PPR gene expression is controlled and regulated by retrograde signals is, however, still unknown. Here, we report a significant role for the general transcription factor TFIIF α-subunit (TFIIFα) in controlling PPR gene expression in Arabidopsis. First, we found that TFIIFα interacts with the BIN4 subunit of the Topoisomerase VI (Topo VI). Transcriptome analysis of TFIIF and Topo VI mutant lines then revealed that many PLS-type PPR genes involved in RNA editing are reciprocally controlled by TFIIF and Topo VI. The misexpression of CLB19 and DYW1 genes in two allelic tfIIfα mutants was associated with editing impairments in their plastid target RNAs rpoA and ndhD, respectively. Interestingly, we also detected a change in NDH activity in tfIIfα plants. We also show that TFIIFα and Topo VI coordinate the expression of NDH subunits encoded by the nuclear and plastid genomes. These results reveal the crucial role of the nuclear TFIIFα and Topo VI complexes in controlling plastid genome expression at multiple levels of regulation, including the particular regulation of PPR gene expression.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

TFIIFα interacts with the Topoisomerase VI complex and selectively controls the expression of genes encoding PPR proteins involved in organellar RNA editing in Arabidopsis

Dimnet¹,

Vriet²,

Achard³

et al. 2022

Preprint

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show abstract

“…Among them, some proteins were reported to have unique functions, including the MEE5 gene, which was associated with embryo development and the development of the female gametophyte [50,51]. In addition, LSM2 [52] and RAP74 [53] regulate transcript splicing and thus influence plant development, and STZ [54] was related to drought stress resistance. These results suggested that the expression bias of the key TFs in the hybrid might be an important factor influencing the global state of transcription and the phenotype.…”

Section: Transcription Factor Analysis Of Non-additive Genesmentioning

confidence: 99%

Transcriptome Shock in Developing Embryos of a Brassica napus and Brassica rapa Hybrid

Zhou,

Zhang,

et al. 2023

IJMS

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Interspecific crosses that fuse the genomes of two different species may result in overall gene expression changes in the hybrid progeny, called ‘transcriptome shock’. To better understand the expression pattern after genome merging during the early stages of allopolyploid formation, we performed RNA sequencing analysis on developing embryos of Brassica rapa, B. napus, and their synthesized allotriploid hybrids. Here, we show that the transcriptome shock occurs in the developing seeds of the hybrids. Of the homoeologous gene pairs, 17.1% exhibit expression bias, with an overall expression bias toward B. rapa. The expression level dominance also biases toward B. rapa, mainly induced by the expression change in homoeologous genes from B. napus. Functional enrichment analysis revealed significant differences in differentially expressed genes (DEGs) related to photosynthesis, hormone synthesis, and other pathways. Further study showed that significant changes in the expression levels of the key transcription factors (TFs) could regulate the overall interaction network in the developing embryo, which might be an essential cause of phenotype change. In conclusion, the present results have revealed the global changes in gene expression patterns in developing seeds of the hybrid between B. rapa and B. napus, and provided novel insights into the occurrence of transcriptome shock for harnessing heterosis.

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Mutagenic effects of ethyl methanesulfonate on nine protein coding genes in tomato (Solanum lycopersicum L.)

Yong

Aswandy

Cheong

et al. 2021

Scientia Horticulturae

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The mutation nrpb1‐A325V in the largest subunit of RNA polymerase II suppresses compromised growth of Arabidopsis plants deficient in a function of the general transcription factor IIF

Cited by 5 publications

References 82 publications

TFIIFα interacts with the Topoisomerase VI complex and selectively controls the expression of genes encoding PPR proteins involved in organellar RNA editing in Arabidopsis

TFIIFα interacts with the Topoisomerase VI complex and selectively controls the expression of genes encoding PPR proteins involved in organellar RNA editing in Arabidopsis

Transcriptome Shock in Developing Embryos of a Brassica napus and Brassica rapa Hybrid

Mutagenic effects of ethyl methanesulfonate on nine protein coding genes in tomato (Solanum lycopersicum L.)

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