The muscle specific domain of mouse N-CAM: structure and alternative splicing patterns

Hamshere, Marion; Dickson, George; Eperon, Ian C.

doi:10.1093/nar/19.17.4709

Cited by 29 publications

(18 citation statements)

References 26 publications

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“…To investigate the functional consequences of blocking miR-133 and up-regulating nPTB and PTB, we assayed the splicing of a number of alternative exons previously shown to change during the course of myogenesis (Hamshere et al 1991;Ziober et al 1993;Herasse et al 1999;Buj-Bello et al 2002). We confirmed the PTB/ nPTB responsiveness of some of these exons as well as the known PTB-dependent exons in SRC and ACTN1 in differentiating C2C12 cells by RNAi knockdown ( Fig.…”

Section: Muscle-specific Micrornas Regulate Nptb Genes and Development 75supporting

confidence: 55%

“…Subsequently, gels were dried and imaged on a Typhoon PhosphorImager (Molecular Dynamics), and bands were quantified using ImageQuant 5.1 software (Molecular Dynamics). Primers used were as follows: nPTB exon 8 forward, 5Ј-GCATTTGCC AAGGAGACATCC-3Ј; nPTB exon 11 reverse, 5Ј-CGCTGCA-CATCTCCATAAACAC-3Ј; PTB exon 8 forward, 5Ј-AAGAG-CAGAGACTACACTCGA-3Ј; PTB exon 12 reverse, 5Ј-CT GCCGTCTGCCATCTGCACAA-3Ј; CAPN3 exon 6 forward, 5Ј-TTCACCAAATCCAACCACCG-3Ј; CAPN3 exon 6 reverse, 5Ј-ACTCCAAGAACCGTTCCACT-3Ј (Herasse et al 1999); NCAM MSD forward (MH1B2 F), 5Ј-CCCCCGCCCC GAATTCCCACTGAGTTCAAGACACAG-3Ј; NCAM MSD reverse (MH2BR), 5Ј-GCCGGCGCGGAGCTTTCTGCCCTT CCAGCTTGGGT-3Ј (Hamshere et al 1991); ITGA7 A7A/B forward:, 5Ј-GCTGCTCAGAGATGCATCC-3Ј; ITGA7 A7A/B reverse, 5Ј-CACCGGATGTCCATCAGGAC-3Ј (Ziober et al 1993); MTMR1 exon 2 forward, 5Ј-CATGTTGAATGGTGTA AACAG-3Ј; MTMR1 exon 5 reverse, 5Ј-AATTATCCCCATG GCTCTGT-3Ј (Buj-Bello et al 2002); MEF2A forward, 5Ј-CTT GATTGGAAATACTGGTGC;-3Ј MEF2A reverse, 5Ј-TCGGA GTTGTCACAGACA-3Ј (Buj-Bello et al 2002).…”

Section: Rt-pcrmentioning

confidence: 99%

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MicroRNAs regulate the expression of the alternative splicing factor nPTB during muscle development

Boutz¹,

Chawla²,

Stoilov³

et al. 2007

Genes Dev.

282

239

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Section: Muscle-specific Micrornas Regulate Nptb Genes and Development 75supporting

confidence: 55%

Section: Rt-pcrmentioning

confidence: 99%

MicroRNAs regulate the expression of the alternative splicing factor nPTB during muscle development

Boutz¹,

Chawla²,

Stoilov³

et al. 2007

Genes Dev.

282

239

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“…We did not detect any hybridization of the MSD probe in brain tissue, perhaps not surprisingly since levels detected by this probe in muscle are already very low. Incorporation of the complete MSD region appears to be the preferred splicing pathway in muscle (Hamshere et al, 1991) with other splice variants being present at low levels.…”

Section: Discussionmentioning

confidence: 99%

Expression of NCAM isoforms during skeletal myogenesis in the mouse embryo

Lyons

Moore

Yahara

et al. 1992

Developmental Dynamics

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We have examined the developmental patterns of neural cell adhesion molecule (NCAM) gene expression in embryonic mouse skeletal muscle cells by in situ hybridization. Moreover, by utilising exon-specific cRNA probes, we have examined tissue specific splicing of the NCAM gene. We show that there is a distinct sequence of NCAM isoform expression during skeletal muscle development. Since NCAMs are also expressed in other cell types, particularly neurons, NCAM mRNAs have been colocalised with acetylcholine receptor a (AChRa) gene transcripts to identify muscle-specific expression. NCAM is first detected in somites as they first form, prior to their differentiation into muscle and nonmuscle compartments. Myotomes, the first skeletal muscle masses to form in the embryo, express mRNAs for the transmembrane 180 and 140 kDa isoforms of NCAM. Both of these transcripts are also detected in the neural tube, and their spatial pattern of expression changes with development. Transcripts containing the muscle-specific domain (MSD) of the NCAM gene are not detected prior to 11 days postcoitum (p.c.1, at a time when rostra1 somites already contain well-developed myotomes. As the level of MSD mRNAs increases at 12 days P.c., the 140 and 180 kDa transcript levels decrease in skeletal muscle masses. The level of all NCAM isoform transcripts declines between 13 and 15 days p.c. in muscle. However, the 180 and 140 kDa NCAM isoforms are expressed at a high level in neural tissue and in other locations in the developing embryo such as in smooth muscle, around vibrissae follicles, and in the perichondrial zone of digits. o 1992 Wiley-Liss, Inc.

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“…The MSDinsert is located in the region between exons 12 and 13, and a homologous insert was detected in NCAM CDNAfrom human and mouse skeletal muscle cells (7,12). While many molecular forms of the NCAM are expressed, major products are three polypeptide chains having desialo-molecular masses of 155 kDa, 145 kDa and 120 kDa in chicken muscle (ref.…”

mentioning

confidence: 99%

Transitional Expression of Neural Cell Adhesion Molecule Isoforms During Chicken Embryonic Myogenesis.

Yoshimi

Mimura

Aimoto

et al. 1993

Cell Struct. Funct.

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Keywords: neural cell adhesion molecule/muscle-specific domain/myogenesis/antipeptide antibody ABSTRUCT. The neural cell adhesion molecule, NCAM, is known to be expressed in chicken muscle as at least three principal molecular forms (molecular masses of 155 kDa, 145 kDa, and 120 kDa). They are generated from a single gene by alternative splicing. To distinguish these molecular species and to investigate their expressions in muscle differentiation during chicken embryonic development, we prepared antipeptide antibodies against three different domains of the NCAM.The antibody named MSD<+ > was designed to detect musclespecific domain (MSD)which was inserted into a muscle-specific NCAM by alternative splicing. The locus encompassing the MSDinsertion site was detected with the antibody named MSD<->, and cytoplasmic sites near the transmembrane region were detected with the antibody named CYT.Immunoblot analysis utilizing the peptide antibodies prepared here revealed that, of three NCAMs, two isoforms, 155 kDa and 120 kDa, were recognized with the antibody MSD<+ > and the same 155-kDa and the other 145-kDa molecule were recognized with the antibody CYT. The antibody MSD<-> was capable of detecting all three isoforms of the NCAM.Expression of the 120-kDa form with MSDbut lacking a cytoplasmic tail increased but that of the 145-kDa form with cytoplasmic tail but lacking MSDdeclined during embryonic day 5 to day 18. The 155-kDa form NCAMwith both MSDand cytoplasmic tail was expressed specifically and transiently during embryonic day ll to day 14 in chick muscle; this period coincides with the period of extensive myotube formation. Thus, this largest isotype seems to play an important role in muscle differentiation.

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The muscle specific domain of mouse N-CAM: structure and alternative splicing patterns

Cited by 29 publications

References 26 publications

MicroRNAs regulate the expression of the alternative splicing factor nPTB during muscle development

MicroRNAs regulate the expression of the alternative splicing factor nPTB during muscle development

Expression of NCAM isoforms during skeletal myogenesis in the mouse embryo

Transitional Expression of Neural Cell Adhesion Molecule Isoforms During Chicken Embryonic Myogenesis.

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