The murine H19 gene is activated during embryonic stem cell differentiation in vitro and at the time of implantation in the developing embryo

Abstract

The differentiation in vitro of murine embryonic stem cells to embryoid bodies mimics events that occur in vivo shortly before and after embryonic implantation. We have used this system, together with differential cDNA cloning, to identify genes the expression of which is regulated during early embryogenesis. Here we describe the isolation of several such cDNA clones, one of which corresponds to the gene H19. This gene is activated in extraembryonic cell types at the time of implantation, suggesting that it may play a role at this stage of development, and is subsequently expressed in all of the cells of the mid-gestation embryo with the striking exception of most of those of the developing central and peripheral nervous systems. After birth, expression of this gene ceases or is dramatically reduced in all tissues.

H19/miR‐30a/C8orf4 axis modulates the adipogenic differentiation process in human adipose tissue‐derived mesenchymal stem cells

“…According to previous studies, H19 was overexpressed during embryonic stem cell differentiation; after birth, it was overexpressed in skeletal muscle and heart (Poirier et al, ), indicating its potential role in cellular differentiation. In fact, H19 reportedly participates in multidifferentiation of mesenchymal stem cells, including myogenic differentiation (Qin, Cai, Qing, Li, & Zhang, ), osteogenic differentiation (Huang et al, ; Huang, Zheng, Jia, & Li, ; Wu et al, ), odonto/osteogenic potential (Yi et al, ), tenogenic differentiation (Lu et al, ), as well as adipogenic differentiation (Huang et al, ).…”

DysregulatedH19/Igf2expression disrupts cardiac-placental axis during development of Silver Russell Syndrome-like mouse models

“…H19 regulates vascular endothelial growth factor (VEGF) in human endothelial cells in vitro (Conigliaro et al, 2015), which is involved in placental angiogenesis, specifically for the branching of fetoplacental vessels beginning at mid-gestation (Woods et al, 2018). Additionally, H19 is highly expressed in trophoblasts (Marsh & Blelloch, 2020; Poirier et al, 1991), and disrupted trophoblast development leads to defective vascular branching in the labyrinth and restricted fetal growth (Ueno et al, 2013). Thus, it is possible that the morphological anomalies observed in the +/ hIC1 placenta are exaggerated by abnormal H19 expression in trophoblasts.…”

Long Non-Coding RNAs in Oral Submucous Fibrosis: Their Functional Mechanisms and Recent Research Progress

“… 58 H19 encodes a 2.3-kb non-coding RNA, is expressed abundantly on almost all maternal alleles in fetal tissues, but its expression drops sharply after birth and it is expressed only in the myocardium and skeletal muscle. 59 , 60 H19 shows high expression in cancers of different tissue origins, owing to the loss of imprinting in some cases. H19 has been shown to have carcinogenic and anti-cancer properties simultaneously.…”