The multiple expression of Ca2+-activated Cl− channels via homo- and hetero-dimer formation of TMEM16A splicing variants in murine portal vein

Modulation of TMEM16A-Channel Activity as Ca2+ Activated Cl− Conductance via the Interaction With Actin Cytoskeleton in Murine Portal Vein

“…At 24 -72 h after transfection, TMEM16A-expressing HEK293 cells elicited CaCC currents, which had characteristics consistent with those of our previous measurements (4). Neither the current density nor the kinetics (t act and t deact ) of CaCC currents were affected by the treatment with 1 mM cytochalasin D for 4 h in TMEM16A(abc)-and TMEM16A(acd)-HEK293 cells (n = 3 -6, P > 0.05; Fig.…”

“…Recently, TMEM16A has been identified as a molecular component responsible for CaCC in various tissues (1 -3). TMEM16A is functionally expressed also in several VSMs including portal vein (4).…”

“…Freshly isolated mPVSMCs were prepared as described previously (4). Electrophysiological studies were performed using a whole cell voltageclamp technique with an EPC-7 amplifier (HEKA Electronics, Darmstadt, Germany), an analog-digital converter (Digidata 1440A; Axon Instruments, Foster City, CA, USA), and pCLAMP software (version 8.2; Axon Instruments).…”

“…1A). These currents were largely blocked by CaCC inhibitors, 100 mM niflumic acid (Sigma-Aldrich, St. Louis, MO, USA) or 10 mM T16A inh -A01 (Tocris Bioscience, Bristol, UK), as reported previously (4). Cytochalasin D (Invitrogen, Carlsbad, CA, USA) was added to inhibit actin polymerization in mPVSMCs.…”

Downregulation of Ca2+-Activated Cl− Channel TMEM16A by the Inhibition of Histone Deacetylase in TMEM16A-Expressing Cancer Cells

“…-activated Cl 2 channel, with eight putative transmembrane domains and cytosolic N-and C-termini (Caputo et al, 2008;Schroeder et al, 2008;Yang et al, 2008), and functions as a dimer (Sheridan et al, 2011;Ohshiro et al, 2014). TMEM16A is located on chromosome 11q13, which is frequently amplified in many types of human cancer (Akervall et al, 1995;Huang et al, 2002), and plays an important role in driving the amplification of 11q13 (Komatsu et al, 2006).…”