The Multiparametric Scanning System for Evaluation of Minimal Residual Disease in Hematological Malignancies

Trakhtenbrot, Luba; Rechavi, Gideon; Amariglio, Ninette

doi:10.1159/000077556

Cited by 14 publications

(9 citation statements)

References 33 publications

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“…Combined morphologic and fluorescent in situ hybridization (FISH) analysis was performed using a new multiparametric cell scanning system (Duet™, BioView Ltd., Rehovot, Israel) [16, 17]. Monoclonality of the T-cells was established by spectratyping for the T-cell receptor gamma rearrangement [18].…”

Section: Methodsmentioning

confidence: 99%

Philadelphia-Chromosome-Positive T-Lymphoblastic Leukemia: Acute Leukemia or Chronic Myelogenous Leukemia Blastic Crisis

et al. 2005

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The Ph1 chromosome has rarely been reported in T-lineage acute lymphoblastic leukemia (T-ALL), and the clinical relevance of this translocation in T-ALL is currently unknown. In chronic myelogenous leukemia (CML) some data indicate derivation of T-cells from the leukemic clone and only a few cases of T-derived blastic crisis have been reported and quite often disputed. Particularly in cases identified initially in blastic crisis it may be difficult to distinguish those from Ph1-positive T-ALL. We herein report 2 patients who presented with a clinical picture of Ph1-positive T-ALL and who raised a differential diagnosis from T-cell blastic crisis of CML. We review the literature and suggest clinical and laboratory features that can help in the diagnosis. According to our literature review, 23 cases of Ph1-positive T-ALL and 44 cases of T-cell blastic crisis of CML, including ours, were reported. Some major differences between the two entities could help in establishing a diagnosis of Ph1-positive T-cell blastic crisis of CML vs. Ph1-positive T-ALL: Male sex and younger age was more predominant in T-ALL. While in most cases of CML blastic crisis there was a history of CML there was no such history in the T-ALL cases. Medullary involvement with lymphoblastic leukemia was present in all cases of T-ALL but only in about half of the cases of CML blastic crisis. None of the CML-blastic crisis cases tested by RT-PCR showed the minor breakpoint transcript, while 2 cases with T-ALL had the minor breakpoint transcript and 1 had both transcripts. Combined morphologic and FISH analysis can help to distinguish between the two entities and was applied in one of our cases. Although both entities carry a severe prognosis, differentiating between them might have clinical relevance, especially in the imatinib era.

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Section: Methodsmentioning

confidence: 99%

Philadelphia-Chromosome-Positive T-Lymphoblastic Leukemia: Acute Leukemia or Chronic Myelogenous Leukemia Blastic Crisis

et al. 2005

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“…Analysis of two I‐FISH experiments was performed by a multiparametric cell scanning Duet TM system (BioView Ltd, Rehovot, Israel) that provides combined analysis of morphology, immunocytochemistry and I‐FISH. In addition, this system enables the combined analysis of two I‐FISH examinations on the same cells (Trakhtenbrot et al , 2004). The technical procedures were previously described in detail (Shimoni et al , 2002).…”

Section: Methodsmentioning

confidence: 99%

Co‐existence of multiple subclones in TEL‐AML1 at diagnosis of acute lymphoblastic leukaemia in association with submicroscopic deletion of AML1

Rothman¹,

Trakhtenbrot²,

Bielorai³

et al. 2005

Br J Haematol

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Summary The TEL/AML1 (ETV6/RUNX1) fusion gene is the most common genetic rearrangement in paediatric acute lymphoblastic leukaemia (ALL). Although considered to be a low‐risk leukaemia, it is associated with a relapse rate of 10–20%. The coexistence of different subclones at diagnosis, based on polymerase chain reaction (PCR) studies of IG/TCR gene rearrangement, with differential response to chemotherapy, was recently reported in this subtype of ALL. We wished to demonstrate such subclones at diagnosis by a recently developed technique of quantitative multiparametric fluorescence in situ hybridization (FISH). Bone marrow cells from 80 paediatric patients with ALL at diagnosis were analysed for the presence of the TEL/AML1 fusion gene by interphase FISH. Fourteen patients were positive for the translocation. Four of them had several subclones associated with various combinations of additional chromosomal abnormalities. The most striking was an atypical and unexpected hybridization pattern consistent with a submicroscopic deletion of the 5′ region of the AML1 breakpoint. Other abnormalities included TEL deletion, trisomy and tetrasomy 21 as well as double TEL‐AML1 fusion. The presence of numerous subclones in about 25% of patients with TEL/AML1+ ALL suggests extensive clonal evolution by the time of diagnosis.

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“…This method has been tested successfully by us and by other authors. 29,30 Chromosome 8, chromosome 17, and MYC were chosen for the current analysis based on the literature and our previous studies. [20][21][22][23]25,31 Chromosome 8 is the site of several genes that have crucial functions in the tumorigenesis of several solid and hematopoietic malignancies.…”

Section: Discussionmentioning

confidence: 99%

Noninvasive detection of aneuploid cells in laryngeal epithelial precursor lesions

Shani

Primov-Fever

Wolf

et al. 2011

Cancer Cytopathology

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BACKGROUND:Most cases of laryngeal cancer are preceded by precursor lesions which, if left untreated, can progress toward an invasive cancer. The objective of this study was to investigate the presence of chromosomal numerical aberrations in cells that were collected by noninvasive brush sampling from laryngeal lesions.METHODS:Laryngeal brush samples from 52 patients were analyzed simultaneously for morphology and fluorescence in situ hybridization (FISH) using centromeric probes for chromosome 17, chromosome 8, and a locus‐specific instability (LSI) v‐myc avian myelocytomatosis viral oncogene homolog (myc) proto‐oncogene protein (C‐MYC) probe for the MYC gene. The patients were divided according to histopathologic diagnosis. Group 1 included patients with squamous cell carcinoma, carcinoma in situ, and severe dysplasia; Group 2 included patients with moderate dysplasia, mild dysplasia, and hyperplasia; and Group 3 included patients with benign nondysplastic lesions.RESULTS:The proportion of cells with MYC and chromosome 8 gains demonstrated significant trends toward being the highest in Group 1 and the lowest in Group 3 (P = .001 and P = .003, respectively). No significant trend was observed for chromosome 17. Mann‐Whitney Bonferroni‐corrected analyses revealed that the most significant contribution was the difference between Groups 1 and 3 (P = .0195 for MYC gains and P = .036 for chromosome 8 gains). When using a cutoff point of 4% aneuploid cells (ACs), both MYC and chromosome 8 differed significantly between groups (P = .030 and P = .037, respectively).CONCLUSIONS:The current results suggested that FISH analysis of brush samples obtained noninvasively from suspicious laryngeal lesions can augment the clinical examination in predicting the nature of the lesions and can aid clinicians in monitoring and follow‐up of high‐risk patients. Cancer (Cancer Cytopathol) 2011. © 2011 American Cancer Society.

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The Multiparametric Scanning System for Evaluation of Minimal Residual Disease in Hematological Malignancies

Cited by 14 publications

References 33 publications

Philadelphia-Chromosome-Positive T-Lymphoblastic Leukemia: Acute Leukemia or Chronic Myelogenous Leukemia Blastic Crisis

Philadelphia-Chromosome-Positive T-Lymphoblastic Leukemia: Acute Leukemia or Chronic Myelogenous Leukemia Blastic Crisis

Co‐existence of multiple subclones in TEL‐AML1 at diagnosis of acute lymphoblastic leukaemia in association with submicroscopic deletion of AML1

Noninvasive detection of aneuploid cells in laryngeal epithelial precursor lesions

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