The multifarious, multireplicon Burkholderia cepacia complex

Mahenthiralingam, Eshwar; Urban, Teresa; Goldberg, Joanna B.

doi:10.1038/nrmicro1085

Cited by 763 publications

(874 citation statements)

References 135 publications

Supporting

Mentioning

837

Contrasting

Unclassified

Order By: Relevance

“…Over the last 20 years, members of the Bcc have emerged as problematic opportunistic human pathogens in immunocompromised individuals and in patients with cystic fibrosis (CF) (Mahenthiralingam et al, 2005). They have the capacity of spreading between patients and lead determined (Holden et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

“…Many pathogenic bacteria, including Burkholderia, have a propensity for varying the surface molecules that interact with their hosts (Rocha & Blanchard, 2002;Mahenthiralingam et al, 2005). A genetic mechanism mediating such phenotype variation involves repetitive DNA sequences that are known to promote genetic variability through homologous and illegitimate recombination processes (Achaz et al, 2002;Bichara et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Genome-wide analysis of DNA repeats in Burkholderia cenocepacia J2315 identifies a novel adhesin-like gene unique to epidemic-associated strains of the ET-12 lineage

2010

View full text Add to dashboard Cite

Members of the Burkholderia cepacia complex (Bcc) are respiratory pathogens in patients with cystic fibrosis (CF). Close repetitive DNA sequences often associate with surface antigens to promote genetic variability in pathogenic bacteria. The genome of Burkholderia cenocepacia J2315, a CF isolate belonging to the epidemic lineage Edinburgh-Toronto (ET-12), was analysed for the presence of close repetitive DNA sequences. Among the 422 DNA close repeats, 45 genes potentially involved in virulence were identified and grouped into 12 classes; of these, 13 genes were included in the antigens class. Two trimeric autotransporter adhesins (TAA) among the 13 putative antigens are absent from the other Burkholderia genomes and are clustered downstream of the cci island that is a marker for transmissible B. cenocepacia strains. This cluster contains four adhesins, one outer-membrane protein, one sensor histidine kinase and two transcriptional regulators. By using PCR, we analysed three genes among 47 Bcc isolates to determine whether the cluster was conserved. These three genes were present in the isolates of the ET-12 lineage but absent in all the other members. Furthermore, the BCAM0224 gene was exclusively detected in this epidemic lineage and may serve as a valuable new addition to the field of Bcc diagnostics. The BCAM0224 gene encodes a putative TAA that demonstrates adhesive properties to the extracellular matrix protein collagen type I. Quantitative real-time PCR analysis indicated that BCAM0224 gene expression occurred preferentially for cells grown under high osmolarity, oxygen-limited conditions and oxidative stress. Inactivation of BCAM0224 in B. cenocepacia attenuates the ability of the mutant to promote cell adherence in vitro and impairs the overall bacterial virulence against Galleria mellonella as a model of infection. Together, our data show that BCAM0224 from B. cenocepacia J2315 represents a new collagen-binding TAA with no bacterial orthologues which has an important role in cellular adhesion and virulence.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Genome-wide analysis of DNA repeats in Burkholderia cenocepacia J2315 identifies a novel adhesin-like gene unique to epidemic-associated strains of the ET-12 lineage

2010

View full text Add to dashboard Cite

show abstract

“…In B. cenocepacia , HGT cannot lead to codon usage bias between any two chromosomes, although it is one of the two most important factors that affect codon usage. HTGs acquired from other bacteria or from the host have substantially contributed to genomic plasticity in B. cenocepacia (Holden et al 2009;Mahenthiralingam et al 2005). Additionally, B. cenocepacia is a species that is successful at balancing the stability and plasticity of the genome organization (Mohn and Schübeler 2009).…”

Section: Discussionmentioning

confidence: 99%

“…Taken together, a substantial portion of the 111 HTGs coincides with the genes, which have been reported to have potential of horizontal transfer to exchange genomic components through functional or nonfunctional accessory genetic elements. Moreover, HTGs in B. cenocepacia have been detected to be substantially contributing to genomic plasticity (Holden et al 2009;Mahenthiralingam et al 2005). Thus, considering the alien origin of these HTGs, it appears that HGT is the major factor of the mutational bias that affects the codon usage variation, and it is responsible for the genomic plasticity of B. cenocepacia .…”

Section: Discussionmentioning

confidence: 99%

Analysis of genomic characters reveals that four distinct gene clusters are correlated with different functions in Burkholderia cenocepacia AU 1054

Yuan

Yang

Ren

et al. 2013

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

Possessing three circular chromosomes is a distinct genomic characteristic of Burkholderia cenocepacia AU 1054, a clinically important pathogen in cystic fibrosis. In this study, base composition, codon usage and functional role category were analyzed in the B. cenocepacia AU 1054 genome. Although no bias in the base and codon usage was detected between any two chromosomes, function differences did exist in the genes of each chromosome. Similar base composition and differential functional role categories indicated that genes on these three chromosomes were relatively stable and that a proper division of labor was established. Based on variations in the base or codon usage, four small gene clusters were observed in all of the genes. Multivariate analysis revealed that protein hydrophobicity played a predominant role in shaping base usage bias, while horizontal gene transfer and the gene expression level were the two most important factors that affected the codon usage bias. Interestingly, we also found that these gene clusters were correlated with different biological functions: (i) 45 pyrimidine-leadingcodon preferred genes were predominantly involved in regulatory function; (ii) most drug resistance-related genes involved in 826 genes that coding for hydrophobic proteins; (iii) most of the 111 horizontal transfer genes were responsible for genomic plasticity; and (iv) 73 highly expressed genes (predicted by their codon adaptation index values) showed environmental adaptation to cystic fibrosis. Our results showed that genes with base or codon usage bias were affected by mutational pressure and natural selection, and their functions could contribute to drug assistance and transmissible activity in B. cenocepacia.

show abstract

“…This resistance appears to be caused by several mechanisms, including the induction of chromosomal β-lactamases [8]. There are at least four β-lactamases encoded in the J2315 genome, (BCAM0393, BCAM1179, BCAM2165 and BCAS0156) and several β-lactamase family proteins with β-lactamase domains [38]. Moreover, these proteins are known to play a role in RND efflux pumps and resistance to antibiotics.…”

Section: Cog R Category: General Function Predictionmentioning

confidence: 99%

RND-4 efflux transporter gene deletion in Burkholderia cenocepacia J2315: a proteomic analysis

Gamberi

Rocchiccioli²,

Papaleo³

et al. 2013

J Proteome Sci Comput Biol

View full text Add to dashboard Cite

Background: The Burkholderia cenocepacia J2315 is Gram-negative bacterium that is a pathogen for cystic fibrosis (CF) patients. It displays a high-level of resistance to most antimicrobial drugs. In Gram-negative bacteria, the ResistanceNodulation-Cell Division (RND) transporter family has a poorly understood role in multidrug resistance. In a previous publication we analysed the RND-4 and RND-9 transporters by microarray analysis. The obtained results suggested that only RND-4 contributes to the antibiotic resistance. The aim of this study was to investigate the role of this efflux transporter from a proteomic point of view.

show abstract

The multifarious, multireplicon Burkholderia cepacia complex

Cited by 763 publications

References 135 publications

Genome-wide analysis of DNA repeats in Burkholderia cenocepacia J2315 identifies a novel adhesin-like gene unique to epidemic-associated strains of the ET-12 lineage

Genome-wide analysis of DNA repeats in Burkholderia cenocepacia J2315 identifies a novel adhesin-like gene unique to epidemic-associated strains of the ET-12 lineage

Analysis of genomic characters reveals that four distinct gene clusters are correlated with different functions in Burkholderia cenocepacia AU 1054

RND-4 efflux transporter gene deletion in Burkholderia cenocepacia J2315: a proteomic analysis

Contact Info

Product

Resources

About