The multifaceted role of quercetin derived from its mitochondrial mechanism

Carrillo-Garmendia, Andres; Madrigal-Perez, Luis Alberto; Regalado-Gonzalez, Carlos

doi:10.1007/s11010-023-04833-w

Cited by 5 publications

(6 citation statements)

References 104 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Oxidative phosphorylation is the main target of quercetin [7]. The redox potential of quercetin is related to its capacity for electron flux disruption on the ETC.…”

Section: Discussionmentioning

confidence: 99%

“…Interestingly, quercetin has also shown toxicity to some organisms like S. cerevisiae [4], mammalian cell lines [5] and bacteria [6]. These apparently opposite phenotypes (beneficial and toxic) could be explained by quercetin interaction with the mitochondria [7], raising the hypothesis that quercetin could impair electron flux in the electron transport chain (ETC), producing a decrease in ATP generation. Furthermore, this idea also suggests that quercetin interaction with the ETC could generate reactive oxygen species (ROS) through quercetin-radical molecules or electron leakage [7].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Pro-oxidant influence of quercetin supplementation inSaccharomyces cerevisiae

Carrillo-Garmendia,

Vaca-Martinez,

Carmona-Moreno

et al. 2024

Preprint

Self Cite

View full text Add to dashboard Cite

How could quercetin exert a pro-survival phenotype (antioxidant) in mammalian cells while toxic to other eukaryotic cells? The redox capacity of quercetin may explain its antioxidant and toxic effects, based on the idea that quercetin impairs the electron transport chain, affecting ATP production and forming quercetin-derived free radicals. Herein, we provide evidence that quercetin supplementation: 1) depolarizes the mitochondrial membrane and augments the ADP/ATP ratio; 2) increases superoxide anion cellular levels; 3) changes the cellular response to H2O2challenge associated with the antioxidant cellular response; 4) sensitizes the cellular response to lipoperoxidation challenge, and 5) augments cardiolipin levels. These events suggest that the quercetin pro-oxidant effect is related to mitochondrial respiration dysfunction and could induce cellular antioxidant response.

show abstract

“…Oxidative phosphorylation is the main target of quercetin [7]. The redox potential of quercetin is related to its capacity for electron flux disruption on the ETC.…”

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Pro-oxidant influence of quercetin supplementation inSaccharomyces cerevisiae

Carrillo-Garmendia,

Vaca-Martinez,

Carmona-Moreno

et al. 2024

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…Despite possible Q release degradation and loss of antioxidant capacity, the antioxidant effect may still be observed, since Q-derived free radicals could induce oxidative stress, increasing cellular production of superoxide anion. As a consequence, antioxidant cellular response is induced, leading to the reduction of total reactive oxygen substances (ROSs) [63]. It is relevant to highlight that the antioxidant capacity reported was that of Q released in the media after SGD and SID and did not involve the total amount of Q loaded into the MSPs.…”

Section: Simulated Gastrointestinal Digestion Of Q-loaded F-mspmentioning

confidence: 99%

Effect of Amine, Carboxyl, or Thiol Functionalization of Mesoporous Silica Particles on Their Efficiency as a Quercetin Delivery System in Simulated Gastrointestinal Conditions

Matadamas-Ortiz,

Pérez-Robles,

Reynoso-Camacho

et al. 2024

Foods

View full text Add to dashboard Cite

Quercetin (Q) dietary supplements exhibit poor oral bioavailability because of degradation throughout gastrointestinal digestion (GD), which may be overcome using mesoporous silica particles (MSPs) as an oral delivery system (ODS). This study aimed to elucidate the effect of the functionalization of MSPs with amine-(A-MSP), carboxyl-(C-MSP), or thiol-(T-MSP) groups on their efficiency as a quercetin ODS (QODS). The type and degree of functionalization (DF) were used as factors in an experimental design. The Q-loaded F-MSP (F-MSP/Q) was characterized by gas physisorption analysis, loading capacity (LC), and dynamic light scattering and kinetics of Q release at gastric and intestinal pHs. Antioxidant capacity and Q concentration of media containing F-MSP/Q were evaluated after simulated GD. A-MSP showed the highest LC (19.79 ± 2.42%). C-MSP showed the lowest particle size at pH 1.5 or 7.4 (≈200 nm). T-MSP exhibited the maximum Q release at pH 7.4 (11.43%). High DF of A-MSP increased Q retention, regardless of pH. A-MSP preserved antioxidant capacity of Q-released gastric media (58.95 ± 3.34%). Nonetheless, MSP and F-MSP did not protect antioxidant properties of Q released in intestinal conditions. C-MSP and T-MSP showed essential features for cellular uptake and Q release within cells that need to be assessed.

show abstract

“…Среди механизмов его антибактериальной активности отмечают действие на мембраны клеток, взаимодействие с белками. Из-за низкой растворимости кверцетина в воде его биологическая активность ограничена низкой биодоступностью [16]. Хинолины, гетероциклические соединения, лежащие в основе ряда алкалоидов, известны своей антибактериальной и антисептической активностью, а также хелатирующими свойствами [17].…”

Section: поиск и разработка новых лекарственных средствunclassified

“…Полученные данные свидетельствуют о том, что, несмотря на антиоксидантные свойства исследуемых БАВ [3], их антибактериальное действие может быть связано с окислительным стрессом. Как уже отмечалось, прооксидантное действие ранее было показано для кверцетина и тимохинона [16,19]. Кроме этого, по крайней мере для кверцетина антибактериальная активность может быть также связана с ДНК-тропностью и повреждением нуклеиновых кислот (активация биолюминесценции E. coli (pRecA-lux) и E. coli (pColD-lux)).…”

Section: поиск и разработка новых лекарственных средствunclassified

Could Bioluminescent Bacteria be Used in the Search for New Plant-derived Antibacterial Substances?

Katsev,

Safronyuk,

Burtseva

et al. 2023

Razrabotka i registraciâ lekarstvennyh sredstv

View full text Add to dashboard Cite

Introduction. Currently, the search for new antibacterial substances is an urgent task due to the growing resistance of pathogens to existing antibiotics. One of the key directions in this area is the expansion of scientific research of medicinal plants, as new sources of therapeutic agents. This article examines the possibility of using highly sensitive bioluminescent test bacteria for these purposes, which can quickly detect non-specific antimicrobial activity and can be adapted to highly effective pharmaceutical screening technologies.Aim. To study the applicability of bioluminescent bacteria for the analysis of the antibacterial activity of biologically active substances (BAS) of plant origin.Materials and methods. BAS quercetin, 8-hydroxyquinoline, gallic acid and thymoquinone, which are often found in medicinal plant raw materials and with which its antibacterial properties are associated, were used in the work. Bacteria with constitutive bioluminescence Aliivibrio fischeri F1 and Escherichia coli (pXen7), as well as recombinant bioreporter strains with inducible luminescence were used as test-objects: E. coli (pRecA-lux), E. coli (pColD-lux), reacting to nucleic acid damage; E. coli (pKatG-lux) and E. coli (pSoxS-lux), sensitive to oxidative stress.Results and discussion. It was found that the nonspecific antimicrobial activity of the studied BAS is manifested in the inhibition of bacterial bioluminescence of test-strains with constitutive glowing. It was noted that the marine test-bacteria A. fischeri F1 have significantly greater sensitivity to the action of BAS, compared with the recombinant strain of E. coli (pXen7). It has been shown that their inhibitory effect begins at concentrations of 2 mcg/ml, and bactericidal activity occurs at concentrations of more than 20 mcg/ml. The results obtained are compared with the data on MIC and MBC of gram(+) and gram(–) pathogens. The study of the induction of bioluminescence of recombinant bioreporter strains showed that the antibacterial effect of the BAS is accompanied by oxidative stress. Also, quercetin caused activation of luminescence in E. coli (pRecA-lux) and E. coli (pColD-lux), which may indicate its participation in damage to nucleic acids. Analysis of the induction factors of bioreporter strains indicates that the revealed mechanisms of antibacterial activity are not major, but may be of a secondary nature.Conclusion. It has been shown that the intensity of the glow of natural and recombinant bioluminescent bacteria can be an indicator of the antibacterial activity of BAS of natural origin. The high sensitivity of A. fischeri F1 bacteria to the action of substances such as quercetin, 8-hydroxyquinoline, gallic acid and thymoquinone has been shown. Considering that bioluminescence analysis is a quantitative instrumental method, it can be easily adapted for high-throughput pharmaceutical screening. It has been shown that the luminescence intensity of natural and recombinant bioluminescent bacteria can be an indicator of the antibacterial activity of BAS of natural origin. The high sensitivity of A. fischeri F1 to the action of substances such as quercetin, 8-hydroxyquinoline, gallic acid and thymoquinone has been established. Taking in an account that bioluminescent analysis is a quantitative instrumental method, it can be easily adapted for high-throughput pharmaceutical screening.

show abstract

The multifaceted role of quercetin derived from its mitochondrial mechanism

Cited by 5 publications

References 104 publications

Pro-oxidant influence of quercetin supplementation inSaccharomyces cerevisiae

Pro-oxidant influence of quercetin supplementation inSaccharomyces cerevisiae

Effect of Amine, Carboxyl, or Thiol Functionalization of Mesoporous Silica Particles on Their Efficiency as a Quercetin Delivery System in Simulated Gastrointestinal Conditions

Could Bioluminescent Bacteria be Used in the Search for New Plant-derived Antibacterial Substances?

Contact Info

Product

Resources

About