The multifaceted<i>C. elegans</i>major sperm protein: an ephrin signaling antagonist in oocyte maturation: Figure 1.

Kuwabara, Patricia E.

doi:10.1101/gad.1061103

Cited by 28 publications

(22 citation statements)

References 36 publications

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“…Major sperm proteins (MSPs) are involved in both extracellular signaling and cytoskeletal functions during reproduction [52]. MSP antagonizes Eph/ephrin signaling, in part, by binding VAB-1 Eph receptor tyrosine kinase on oocytes and sheath cells to promote oocyte maturation and MAPK activation [53,54]. MSP proteins assemble into fibrous networks that drive movement of the C. elegans sperm [55].…”

Section: Discussionmentioning

confidence: 99%

Gene expression profiling of oxidative stress response of C. elegans aging defective AMPK mutants using massively parallel transcriptome sequencing

et al. 2011

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BackgroundA strong association between stress resistance and longevity in multicellular organisms has been established as many mutations that extend lifespan also show increased resistance to stress. AAK-2, the C. elegans homolog of an alpha subunit of AMP-activated protein kinase (AMPK) is an intracellular fuel sensor that regulates cellular energy homeostasis and functions in stress resistance and lifespan extension.FindingsHere, we investigated global transcriptional responses of aak-2 mutants to oxidative stress and in turn identified potential downstream targets of AAK-2 involved in stress resistance in C. elegans. We employed massively parallel Illumina sequencing technology and performed comprehensive comparative transcriptome analysis. Specifically, we compared the transcriptomes of aak-2 and wild type animals under normal conditions and conditions of induced oxidative stress. This research has presented a snapshot of genome-wide transcriptional activities that take place in C. elegans in response to oxidative stress both in the presence and absence of AAK-2.ConclusionsThe analysis presented in this study has enabled us to identify potential genes involved in stress resistance that may be either directly or indirectly under the control of AAK-2. Furthermore, we have extended our current knowledge of general defense responses of C. elegans against oxidative stress supporting the function for AAK-2 in inhibition of biosynthetic processes, especially lipid synthesis, under oxidative stress and transcriptional regulation of genes involved in reproductive processes.

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Section: Discussionmentioning

confidence: 99%

Gene expression profiling of oxidative stress response of C. elegans aging defective AMPK mutants using massively parallel transcriptome sequencing

et al. 2011

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“…The overall reproductive output is primarily determined by the availability of sperm [34], [40], because their number is set for the lifetime of an individual. Many of the specific molecular components involved in gametogenesis and later reproductive events have been characterized [41], [42], [43], [44], [45], [46], [47]. For example, a signaling mechanism directly couples oocyte maturation and ovulation to the presence of sperm [31], [32], [48].…”

Section: Introductionmentioning

confidence: 99%

Macro-level Modeling of the Response of C. elegans Reproduction to Chronic Heat Stress

et al. 2012

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A major goal of systems biology is to understand how organism-level behavior arises from a myriad of molecular interactions. Often this involves complex sets of rules describing interactions among a large number of components. As an alternative, we have developed a simple, macro-level model to describe how chronic temperature stress affects reproduction in C. elegans. Our approach uses fundamental engineering principles, together with a limited set of experimentally derived facts, and provides quantitatively accurate predictions of performance under a range of physiologically relevant conditions. We generated detailed time-resolved experimental data to evaluate the ability of our model to describe the dynamics of C. elegans reproduction. We find considerable heterogeneity in responses of individual animals to heat stress, which can be understood as modulation of a few processes and may represent a strategy for coping with the ever-changing environment. Our experimental results and model provide quantitative insight into the breakdown of a robust biological system under stress and suggest, surprisingly, that the behavior of complex biological systems may be determined by a small number of key components.

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“…The identification of MSP's signaling role posed the problem of how oocytes and sheath cells sense MSP. Key advances were the identification of the VAB‐1 Eph receptor protein‐tyrosine kinase as an MSP receptor, and the finding that MSP functions as an ephrin signaling antagonist (Miller et al,2003; reviewed by Kuwabara,2003). Recent results show that the VAB‐1 ectodomain directly binds MSP in vitro (H. Cheng and D. Greenstein, unpublished results).…”

Section: Meiotic Maturation In C Elegansmentioning

confidence: 99%

Start me up: Cell signaling and the journey from oocyte to embryo in C. elegans

Yamamoto

Kosinski

Greenstein

2005

Developmental Dynamics

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Intercellular communication plays a pivotal role in regulating and coordinating oocyte meiosis and fertilization, key triggers for embryonic development. The nematode Caenorhabaditis elegans has emerged as an important experimental paradigm for exploring these fundamental reproductive processes and their regulation. The oocytes of most animal species arrest during meiotic prophase and complete meiosis in response to intercellular signaling in the process of meiotic maturation. Oocyte meiotic maturation is defined by the transition between diakinesis and metaphase of meiosis I and is accompanied by nuclear envelope breakdown and meiotic spindle assembly. As such, the meiotic maturation process is essential for completing meiosis and a prerequisite for successful fertilization. In C. elegans, the processes of meiotic maturation, ovulation, and fertilization are temporally coupled: sperm utilize the major sperm protein as a hormone to trigger oocyte meiotic maturation, and, in turn, the maturing oocyte signals its own ovulation, leading to fertilization. The powerful genetic screens possible in C. elegans have led to the identification of several sperm cell surface proteins that are required for the interaction and fusion of gametes at fertilization. The study of these proteins provides fundamental insights into fertilization mechanisms, their role in speciation, and their potential conservation across phyla. Signaling processes sparked by fertilization are required for meiotic chromosome segregation and initiating the embryonic program. Here we review recent advances in understanding how signaling mechanisms contribute to the oocyte-to-embryo transition in C. elegans. Developmental Dynamics 235:571-585, 2006.

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The multifacetedC. elegansmajor sperm protein: an ephrin signaling antagonist in oocyte maturation: Figure 1.

Cited by 28 publications

References 36 publications

Gene expression profiling of oxidative stress response of C. elegans aging defective AMPK mutants using massively parallel transcriptome sequencing

Gene expression profiling of oxidative stress response of C. elegans aging defective AMPK mutants using massively parallel transcriptome sequencing

Macro-level Modeling of the Response of C. elegans Reproduction to Chronic Heat Stress

Start me up: Cell signaling and the journey from oocyte to embryo in C. elegans

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