The Mucin Box and Signal/Anchor Sequence of Rat Neutral Ceramidase Recruit Bacterial Sphingomyelinase to the Plasma Membrane

Nakagawa, Tetsuto; Tani, Motohiro; Sueyoshi, Noriyuki; Ito, Makoto

doi:10.1271/bbb.100767

Cited by 2 publications

(1 citation statement)

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“…Ncdase containing High mannose type N-glycans transported to golgi apparatus from ER region to form a complex with Mucins, where the mucins are highly O glycosylated. It helps the Ncdase to retain its integral membrane structure in the raft region [7].and also mucin expression provides a protective barrier against pathogenic bacterial colonization [6] In this communication an attempt to resolve the evolutionary relationship between the NCDases in different organisms which was made by comparing the sequences among different classes and presence of highly conserved domain also a CDase active site with possible role in reverse CDase activity and Mucin Box a specific Serine -Threoinine rich domain in the sequences Insilco.…”

Section: Introductionmentioning

confidence: 99%

Phylogenetic Analysis of Neutral Ceramidase

R¹,

Auxillia²

2014

IJCA

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Neutral ceramidase (NCDase) plays an important role in cell regulation, and has been observed in most of the organism across different groups. In this study the molecular evolution of NCDase in different organisms was studied. The phylogenetic tree relationships among the organisms were explored by comparing the sequences of their NCDase. Mulitle sequence alignment of NCDase protein sequence of different source organisms revealed sequence level homology and highly conserved regions level. A conserved hexapeptide sequence GDVSPN within a large conserved domain was observed frequently in the sequences studies, further study of the sequences indicated the presence of Ser/Thr rich mucin like domains in the sequences of vertebrate NCDase enzymes, but was totally absent in invertebrates and bacteria, confirming the specific domain was acquired separately in mammals during the course of evolution. Comparisons of similarity values and inspection of phylogenetic tree was used to better trace the evolutionary history of Neutral ceramidase and resolve relationships between the organisms.

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Section: Introductionmentioning

confidence: 99%