The MTT assay for chemosensitivity testing of human tumors of the central nervous system

Abstract

In this study we assessed the influence of patient- and drug-specific parameters in the short-term MTT-chemosensitivity assay in 150 primary cell cultures derived from human brain tumors. In 45 patients the MTT assay was directly compared with the CFA (Colony Forming Assay). Resistance was 10-20% higher in the MTT assay than in the CFA, but there was a good agreement in both assays, that more malignant gliomas had a higher in vitro chemosensitivity against ACNU and BCNU. Overall the results demonstrate, that there is no uniform correlation between the in vitro chemosensitivity and the histopathological classification of the tumors, which corresponds well to the clinical situation. On the basis of this study we suggest prospective clinical trials with the MTT assay in human brain tumors.

Flow Cytometry Analysis of Neural Differentiation Markers Expression in Human Glioblastomas May Predict Their Response to Chemotherapy

“…Bogdahn (1983) reported that seven patients with gliomas showing in vitro sensitivity to BCNU studied with a thymidine uptake assay lived longer than the ten patients who did not. The study was in contrast to the results reported by Nikkhah et al (1992) that BCNU sensitivity increased with increasing grades of malignancy. In vitro chemosensitivity assay should provide a tool for selection Cell Mol Neurobiol (2009) 29:845-858 855 of a specific drug for the individual patient.…”

Contribution of O6‐methylguanine‐DNA methyltransferase to monofunctional alkylating‐agent resistance in human brain tumor—derived cell lines

“…When nine brain tumor lines and HT-29 were exposed to MNNG in suspension and then plated on a plastic surface to grow as adherent cultures, survival of ability to reduce MTT [34,35] was essentially the same as the survival of soft-agar colony-forming ability reported in Table 2. DT, D37, and LDlo in the absence and presence of 06-BG were, on average, within 9% of the values in Table 2 and differed by no more than 30%.2 These additional assays further demonstrate the reproducibility of survival data for cells methylated in suspension.…”

“…We also assayed the MNNG sensitivity of adherent cells by using reduction of the colored formazan derivative MTT [34,35] rather than colony-forming ability as a measure of cell viability. For the eight lines (including HT-29) assayed by using MTT reduction as an end point, the results were essentially the same as obtained by measuring survival of colony-forming ability, both in the absence and presence of 06-BG.…”

Cytotoxicity of gemcitabine enhanced by polyphenolics fromAronia melanocarpain pancreatic cancer cell line AsPC-1

“…MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide) is reduced to insoluble formazan by the mitochondrial succinate dehydrogenase of viable cells 18. The formazan generated is dissolved in DMSO and the colour quantified by spectrophotometry at 570 nm.…”