The MSS51 gene product is required for the translation of the COX1 mRNA in yeast mitochondria

Decoster, Estelle; Simon, Michel; Hatat, D.; Faye, Gérard

doi:10.1007/bf00259457

Cited by 92 publications

(76 citation statements)

References 25 publications

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“…Polypeptide from a COX1 Transcript in mss51 Null Mutants PET309 and MSS51 code for inner membrane proteins that are essential for translation of mitochondrial COX1 mRNA in S. cerevisiae (Decoster et al, 1990;Manthey and McEwen, 1995). In agreement with earlier studies, mutations in either of these genes abolish synthesis of Cox1p ( Figure 1A).…”

Section: Synthesis Of a Novelsupporting

confidence: 90%

“…Mss51p has been conjectured to act on the COXI mRNA 5Ј-UTR to initiate translation and on the coding sequence to promote elongation (Decoster et al, 1990;Perez-Martinez et al, 2003). Because mp15 is only detected in the W303 and D273 mtDNA backgrounds, it is conceivable that the presence of a particular sequence(s) in the 5Ј-UTR of COX1 of these strains could by-pass the requirement of Mss51p for translation initiation but not elongation.…”

Section: Mss51p Binds To the 5 -Utr Of Cox1 Mrnamentioning

confidence: 99%

“…Translation of COX1 in S. cerevisiae is under the control of the MSS51 and PET309 gene products that are also involved in maturation of the COX1 mRNAs (Decoster et al, 1990;Manthey and McEwen, 1995). Mutations in MSS51 or overexpression of the wild type gene can suppress shy1 null mutants by enhancing synthesis of Cox1p (Barrientos et al, 2002b).…”

Section: Introductionmentioning

confidence: 99%

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Aberrant Translation of CytochromecOxidase Subunit 1 mRNA Species in the Absence of Mss51p in the YeastSaccharomyces cerevisiae

Zambrano

Fontanesi

Solans

et al. 2007

MBoC

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Expression of yeast mitochondrial genes depends on specific translational activators acting on the 5 -untranslated region of their target mRNAs. Mss51p is a translational factor for cytochrome c oxidase subunit 1 (COX1) mRNA and a key player in down-regulating Cox1p expression when subunits with which it normally interacts are not available. Mss51p probably acts on the 5 -untranslated region of COX1 mRNA to initiate translation and on the coding sequence itself to facilitate elongation. Mss51p binds newly synthesized Cox1p, an interaction that could be necessary for translation. To gain insight into the different roles of Mss51p on Cox1p biogenesis, we have analyzed the properties of a new mitochondrial protein, mp15, which is synthesized in mss51 mutants and in cytochrome oxidase mutants in which Cox1p translation is suppressed. The mp15 polypeptide is not detected in cox14 mutants that express Cox1p normally. We show that mp15 is a truncated translation product of COX1 mRNA whose synthesis requires the COX1 mRNA-specific translational activator Pet309p. These results support a key role for Mss51p in translationally regulating Cox1p synthesis by the status of cytochrome oxidase assembly.

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Section: Synthesis Of a Novelsupporting

confidence: 90%

Section: Mss51p Binds To the 5 -Utr Of Cox1 Mrnamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Aberrant Translation of CytochromecOxidase Subunit 1 mRNA Species in the Absence of Mss51p in the YeastSaccharomyces cerevisiae

Zambrano

Fontanesi

Solans

et al. 2007

MBoC

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“…An essential feature of this mechanism is that a factor needed for translation of a specific mRNA also binds to the newly synthesized but unassembled protein product. For example, Mss51p, a COX1-specific translation factor (22) was shown to bind to Cox1p before incorporation of this subunit into cytochrome oxidase (23). The release of the factor from the complex, a necessary step for initiating a new round of translation, occurs after the newly translated subunit interacts with a partner protein imported from the cytoplasm.…”

Section: Discussionmentioning

confidence: 99%

F ₁ -dependent translation of mitochondrially encoded Atp6p and Atp8p subunits of yeast ATP synthase

Rak

Tzagoloff

2009

Proc. Natl. Acad. Sci. U.S.A.

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The ATP synthase of yeast mitochondria is composed of 17 different subunit polypeptides. We have screened a panel of ATP synthase mutants for impaired expression of Atp6p, Atp8p, and Atp9p, the only mitochondrially encoded subunits of ATP synthase. Our results show that translation of Atp6p and Atp8p is activated by F1 ATPase (or assembly intermediates thereof). Mutants lacking the ␣ or ␤ subunits of F1, or the Atp11p and Atp12p chaperones that promote F1 assembly, have normal levels of the bicistronic ATP8/ATP6 mRNAs but fail to synthesize Atp6p and Atp8p. F1 mutants are also unable to express ARG8 m when this normally nuclear gene is substituted for ATP6 or ATP8 in mitochondrial DNA. Translational activation by F1 is also supported by the ability of ATP22, an Atp6p-specific translation factor, to restore Atp6p and to a lesser degree Atp8p synthesis in the absence of F1. These results establish a mechanism by which expression of ATP6 and ATP8 is translationally regulated by F1 to achieve a balanced output of two compartmentally separated sets of ATP synthase genes.F1-ATPase ͉ mitochondria ͉ Saccharomyces cerevisiae ͉ translational regulation

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“…Recently, we along with others have proposed an additional level of regulation, namely, an assembly-controlled synthesis of Cox1 (1,2,39,56). In S. cerevisiae, COX1 mRNA translation is under the control of Mss51 and Pet309 (8,30). Mss51 is a key element of the regulatory system.…”

mentioning

confidence: 99%

Mss51 and Ssc1 Facilitate Translational Regulation of Cytochrome c Oxidase Biogenesis

Fontanesi¹,

Soto

Horn

et al. 2010

Molecular and Cellular Biology

122

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The intricate biogenesis of multimeric organellar enzymes of dual genetic origin entails several levels of regulation. In Saccharomyces cerevisiae, mitochondrial cytochrome c oxidase (COX) assembly is regulated translationally. Synthesis of subunit 1 (Cox1) is contingent on the availability of its assembly partners, thereby acting as a negative feedback loop that coordinates COX1 mRNA translation with Cox1 utilization during COX assembly. The COX1 mRNA-specific translational activator Mss51 plays a fundamental role in this process. Here, we report that Mss51 successively interacts with the COX1 mRNA translational apparatus, newly synthesized Cox1, and other COX assembly factors during Cox1 maturation/assembly. Notably, the mitochondrial Hsp70 chaperone Ssc1 is shown to be an Mss51 partner throughout its metabolic cycle. We conclude that Ssc1, by interacting with Mss51 and Mss51-containing complexes, plays a critical role in Cox1 biogenesis, COX assembly, and the translational regulation of these processes.

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The MSS51 gene product is required for the translation of the COX1 mRNA in yeast mitochondria

Cited by 92 publications

References 25 publications

Aberrant Translation of CytochromecOxidase Subunit 1 mRNA Species in the Absence of Mss51p in the YeastSaccharomyces cerevisiae

Aberrant Translation of CytochromecOxidase Subunit 1 mRNA Species in the Absence of Mss51p in the YeastSaccharomyces cerevisiae

F ₁ -dependent translation of mitochondrially encoded Atp6p and Atp8p subunits of yeast ATP synthase

Mss51 and Ssc1 Facilitate Translational Regulation of Cytochrome c Oxidase Biogenesis

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The MSS51 gene product is required for the translation of the COX1 mRNA in yeast mitochondria

Cited by 92 publications

References 25 publications

Aberrant Translation of CytochromecOxidase Subunit 1 mRNA Species in the Absence of Mss51p in the YeastSaccharomyces cerevisiae

Aberrant Translation of CytochromecOxidase Subunit 1 mRNA Species in the Absence of Mss51p in the YeastSaccharomyces cerevisiae

F 1 -dependent translation of mitochondrially encoded Atp6p and Atp8p subunits of yeast ATP synthase

Mss51 and Ssc1 Facilitate Translational Regulation of Cytochrome c Oxidase Biogenesis

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F ₁ -dependent translation of mitochondrially encoded Atp6p and Atp8p subunits of yeast ATP synthase