The mRNA expressions and immunohistochemistry of factors involved in angiogenesis and lymphangiogenesis in the early stage of rat skin incision wounds

Kameyama, Hiroshi; Udagawa, Orie; Hoshi, Tomoaki; Toukairin, Yoko; Arai, Tomomi; Nogami, Makoto

doi:10.1016/j.legalmed.2015.02.007

Cited by 18 publications

(16 citation statements)

References 71 publications

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“…PDGFA, MET, MMP9 and SERPINE1 have been identified as the most promising genes involved in those processes based on miRNA gene expression analysis and literature data (19). PDGFA is involved in angiogenesis, while MET, MMP9 and SERPINE1 are involved in the invasion and metastasis of cancer (20,21).…”

Section: Introductionmentioning

confidence: 99%

Expression of MMP9, SERPINE1 and miR-134 as prognostic factors in esophageal cancer

et al. 2016

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Abstract. Esophageal cancer (EC) is a malignant tumor with a typically poor prognosis for patients. It is well known that certain microRNA (miRNA/miR) genes can regulate other genes responsible for carcinogenesis. In the present study, a group of these genes (miR-21, miR-134, miR-205 and miR-495) and genes connected with cancer-related pathways (MET, MMP9, PDGFA and SERPINE1) were chosen for analysis in order to find a potential correlation between their expression and the clinicopathological factors of EC. Esophageal tumors and adjacent non-cancerous tissue specimens were collected from a total of 63 patients and embedded in paraffin. Commercial arrays were used on KYSE-30, KYSE-150 and KYSE-270 EC cell lines in order to find genes of different expression profiles compared with those acquired from the control Het-1A cell line. Quantitative polymerase chain reaction was used on formalin-fixed, paraffin-embedded samples in order to analyze the expression of the genes chosen in the earlier step. The results were analyzed by the Kruskal-Wallis and Mann-Whitney U tests, Spearman's rank correlation coefficient, Kaplan-Meier methods and the long-rank test. Only miR-495 was not expressed in the analyzed samples. The expression of MMP9 and SERPINE1 was significantly coefficient with age range (P=0.011 and P=0.044, respectively) according to the Kruskal-Wallis test. The Spearman's rank-order correlation measurement showed that there was a coefficient correlation between age and miR-134 expression. The same measurement demonstrated a correlation between age range and MMP9 expression. The expression of miR-134 and MMP9 were also found to be correlated. In all cases, a value of P<0.049 was recorded. Overall, the present study demonstrated that MMP9, SERPINE1 and miR-134 were the most prognostic genes in Caucasian patients with EC.

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Section: Introductionmentioning

confidence: 99%

Expression of MMP9, SERPINE1 and miR-134 as prognostic factors in esophageal cancer

et al. 2016

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“…We have previously reported the mRNA expression levels of various angiogenic factors found in wound tissues at 24 hours after a skin incision [13]. Therefore, for this study we also chose the 24 hour as a time point after the skin incision to measure the mRNA expression levels in tissues at various Zeitegeber time (ZT) points [13].…”

Section: Skin Incisionmentioning

confidence: 99%

“…Therefore, for this study we also chose the 24 hour as a time point after the skin incision to measure the mRNA expression levels in tissues at various Zeitegeber time (ZT) points [13]. Of note, ZT is a 12 hour light-12 hour dark cycle, with ZT0 representing the time when lights are turned on and ZT12 the time when lights are off.…”

Section: Skin Incisionmentioning

confidence: 99%

“…The relative expressions of various angiogenic and lymphagiogenetic factors were determined by TaqMan Gene Expression Assay and TaqMan Fast Virus 1-Step Master Mix (Fischer Scientific, USA), using the StepOne Plus (Fischer Scientific, USA) real-time PCR system [13]. The factors studied were as follows with TaqMan Gene Expression Assay primer IDs in parentheses :…”

Section: Semi-quantification Of Angiogenic Factor Mrnas By Real Time mentioning

confidence: 99%

“…We then hypothesized that angiogenic factors produced by leucocytes and other infiltrating cells in the wound area are under the influence of per2. To test this hypothesis, we applied the incision wound model to per2-mutant and control mice to examine the mRNA expression levels of angiogenic factors: VEGF-A (VEGF), hepatocyte growth factor (HGF), HIF1a, fibroblast growth factor 1 (FGF1), angiopoietin 1 and 2 (ANGPT1 and 2), angiopoietinlike proteins 1 and 2 (ANGPTL1 and 2), PAI1, granulocyte colony stimulating factor (CSF3/G-CSF), chemokine (C-X-C motif) ligand2 (CXCL2), matrix metallopeptidase 9 (MMP9), transforming growth factor beta 1 (TGFb1), colony-stimulating factor 2 (CSF2), and antiangiogenic factor tenomodulin (TNMD) [12,13].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Circadian rhythms of angiogenic factors in skin and wound tissue in per2-mutant mice

Hoshi¹,

Toukairin²,

Arai³

et al. 2017

Biomed Res Clin Prac

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It has recently been reported that circadian gene Period 2 (per2) regulates mRNA expression of vascular endothelial growth factor gene (Vegf) in some tumor cells. It still remains unknown, however, whether per2 has an effect on expression of angiogenic factors during the wound healing process. To investigate the circadian rhythms of angiogenic factors in intact skin and wound tissue in per2-mutant and control mice, we used a skin incision wound model on per2-mutant and control mice, and analyzed circadian rhythms of mRNA levels for several angiogenic factors. Our results show a novel finding that, for some angiogenic factors, the circadian rhythms of mRNA levels in wound tissue exist in per2-mutant as well as in control mice, indicating that some circadian rhythms may be per2-independent. We observed circadian rhythms of per2 and some angiogenic factor mRNAs in control mouse intact skin. The circadian patterns of some angiogenic factors mRNAs in intact skin were altered in per2-mutant mice relative to normal mice, indicating the existence of per2-dependent and independent regulations of angiogenic factors expressions.

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Emerging roles and mechanisms of FOXC2 in cancer

Wang

Zheng

Wang

et al. 2018

Clinica Chimica Acta

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The mRNA expressions and immunohistochemistry of factors involved in angiogenesis and lymphangiogenesis in the early stage of rat skin incision wounds

Cited by 18 publications

References 71 publications

Expression of MMP9, SERPINE1 and miR-134 as prognostic factors in esophageal cancer

Expression of MMP9, SERPINE1 and miR-134 as prognostic factors in esophageal cancer

Circadian rhythms of angiogenic factors in skin and wound tissue in per2-mutant mice

Emerging roles and mechanisms of FOXC2 in cancer

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