The mRNA expression and promoter methylation status of the p16 gene in colony-forming cells with high proliferative potential in patients with psoriasis

Zhang, K.; Zhang, R.; Li, X.; Yin, Guohua; Niu, Xuping; Hou, Ruixia

doi:10.1111/j.1365-2230.2007.02458.x

Cited by 39 publications

(26 citation statements)

References 28 publications

(48 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[23], indicating that the hematopoietic microenvironment of the bone marrow in patients with psoriasis was aberrant. In vitro culture of psoriatic bone marrow-derived hematopoietic stem cells indicated that the patient-derived hematopoietic cells had decreased colony formation ability [21,24]. CD4+/CD8+ T cells and CD4+CD25+ regulatory T cells derived from psoriatic bone marrow CD34+ cells in vitro have been found to be functionally similar to those circulating and lesional psoriatic T cells [6,7].…”

Section: Discussionmentioning

confidence: 99%

Expression of Notch Receptor and Its Target Gene Hes-1 in Bone Marrow CD34+ Cells from Patients with Psoriasis

Yin

Hou

et al. 2012

Dermatology

View full text Add to dashboard Cite

Psoriasis is an autoimmune disease mediated mainly by dysfunctional peripheral blood T cells. Both CD4+/CD8+ T cells and CD4+CD25+ regulatory T cells derived from psoriatic CD34+ bone marrow cells in vitro have been found to be functionally similar to those psoriatic circulating and lesional T cells. Notch signaling participates in diverse cell fate decisions during T cell development and has been reported to influence the proliferation of hematopoietic stem cells and the differentiation of T cells. The purpose of this study was to investigate the expression levels of Notch receptor 1, 2 and its target gene Hes-1 in CD34+ cells from patients with psoriasis. The total RNA and protein of CD34+ cells were extracted, and the mRNA as well as protein expression of Notch1, Notch2 and Hes-1 were investigated using real-time PCR and Western blot assays. We found that the mRNA and protein expression levels of Notch1 and Hes-1 in psoriasis patients were higher compared to normal controls, while the Notch2 mRNA and protein expression levels in psoriasis patients were similar to those of normal controls. The elevated Notch1 and Hes-1 expression levels in psoriatic CD34+ cells might be one reason for the immune disorders which are mainly mediated by T cells.

show abstract

Section: Discussionmentioning

confidence: 99%

Expression of Notch Receptor and Its Target Gene Hes-1 in Bone Marrow CD34+ Cells from Patients with Psoriasis

Yin

Hou

et al. 2012

Dermatology

View full text Add to dashboard Cite

show abstract

“…This finding seemed to be biologically plausible, as MTHFR is associated with DNA methylation reactions (Stern et al, 2000) and DNA methylation disorders might play a role in the etiopathogenesis of psoriasis (Ruchusatsawat et al, 2006;Zhang et al, 2007). Moreover, a lower frequency of the tyrosine phosphatase (SHP-1) gene and p16 gene demethylation has been observed in psoriatic skin lesions (Ruchusatsawat et al, 2006;Zhang et al, 2007). However, several case-control studies carried out subsequently have reported inconsistent results (Weger et al, 2008;Vasku et al, 2009;Liew et al, 2012;Asefi et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Association between MTHFR 677C/T polymorphism and psoriasis risk: a meta-analysis

Shi

Chen

et al. 2015

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. Previous studies investigating the association between methylenetetrahydrofolate reductase (MTHFR) 677C/T polymorphisms and psoriasis risk have reported inconsistent results. The present metaanalysis aimed to comprehensively evaluate the association between MTHFR 677C/T polymorphism and psoriasis risk. The studies regarding the association between MTHFR 677C/T polymorphism and psoriasis risk were retrieved from the PubMed, Embase, Web of Science, Chinese National Knowledge Infrastructure, and Chinese Biomedical databases. Data were extracted and statistical analysis was performed with the program STATA 12.0. A total of seven studies involving 1290 psoriasis cases and 1068 healthy controls were retrieved. Combined analysis showed that there was no significant difference in MTHFR 677C/T genotype distribution between psoriasis and control subjects in the comparisons C vs T, CC vs CT + TT, CC + CT vs TT, CC vs TT, Subgroup analysis by ethnicity also showed no significant association between MTHFR 677C/T polymorphism and psoriasis risk in both Asian and Caucasian populations. In conclusion, this meta-analysis indicates that MTHFR 677C/T polymorphism may not be associated with psoriasis risk.

show abstract

“…Similarly, hypomethylation of p16INK4A (antiapoptotic gene) has been reported in psoriasis, along with demethylation of one of the promoters of the SHP-1 gene, important for keratinocyte growth and proliferation (Ruchusatsawat et al 2006;Zhang et al 2007;Chen et al 2008). Importantly, the findings from these studies provides additional evidence that the molecular defects underlying polygenic skin diseases transcend single-gene mutations, and may be characterized by more global changes at the chromatin level associated with a multitude of downstream effects on gene expression.…”

Section: Aberrant Dna Methylation In Autoimmunerelated Skin Diseasesmentioning

confidence: 56%

The Genetics of Human Skin Disease

DeStefano

Christiano

2014

Cold Spring Harbor Perspectives in Medicine

View full text Add to dashboard Cite

The skin is composed of a variety of cell types expressing specific molecules and possessing different properties that facilitate the complex interactions and intercellular communication essential for maintaining the structural integrity of the skin. Importantly, a single mutation in one of these molecules can disrupt the entire organization and function of these essential networks, leading to cell separation, blistering, and other striking phenotypes observed in inherited skin diseases. Over the past several decades, the genetic basis of many monogenic skin diseases has been elucidated using classical genetic techniques. Importantly, the findings from these studies has shed light onto the many classes of molecules and essential genetic as well as molecular interactions that lend the skin its rigid, yet flexible properties. With the advent of the human genome project, next-generation sequencing techniques, as well as several other recently developed methods, tremendous progress has been made in dissecting the genetic architecture of complex, non-Mendelian skin diseases.

show abstract

The mRNA expression and promoter methylation status of the p16 gene in colony-forming cells with high proliferative potential in patients with psoriasis

Cited by 39 publications

References 28 publications

Expression of Notch Receptor and Its Target Gene Hes-1 in Bone Marrow CD34+ Cells from Patients with Psoriasis

Expression of Notch Receptor and Its Target Gene Hes-1 in Bone Marrow CD34+ Cells from Patients with Psoriasis

Association between MTHFR 677C/T polymorphism and psoriasis risk: a meta-analysis

The Genetics of Human Skin Disease

Contact Info

Product

Resources

About