The MP65 gene is required for cell wall integrity, adherence to epithelial cells and biofilm formation in Candida albicans

Sandini, Silvia; Stringaro, Annarita; Arancia, Silvia; Colone, Marisa; Mondello, Francesca; Murtas, Susanna; Girolamo, Antonietta; Mastrangelo, Nicolina; Bernardis, Flavia De

doi:10.1186/1471-2180-11-106

Cited by 55 publications

(48 citation statements)

References 53 publications

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“…There is an adhesin-like protein expressed at highest levels in yeast cells, Ywp1 ( Y east W all P rotein 1), but it seems to function as an anti-adhesin (38). There are also proteins that may function as adhesins but lack a GPI anchor, including Mp65 ( M anno p rotein of 65 kDa) (39), Csh1 ( C ell S urface H ydrophobicity) (40) and Pra1 ( p H r egulated a ntigen) (32). …”

Section: The Cell Surface and Adherencementioning

confidence: 99%

Candida albicans Biofilm Development and Its Genetic Control

Desai

Mitchell

2015

Microbiol Spectr

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The fungus Candida albicans is a major source of device-associated infection because of its capacity for biofilm formation. It is part of the natural mucosal flora, and thus has access to available niches that can lead to infection. In this chapter we discuss the major properties of C. albicans biofilms, and the insight that has been gleaned from their genetic determinants. Our specific areas of focus include biofilm structure and development, cell morphology and biofilm formation, biofilm-associated gene expression, the cell surface and adherence, the extracellular matrix, biofilm metabolism, and biofilm drug resistance.

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Section: The Cell Surface and Adherencementioning

confidence: 99%

Candida albicans Biofilm Development and Its Genetic Control

Desai

Mitchell

2015

Microbiol Spectr

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“…Of these differential responses in the two biofilm phenotypes, a vast number of genes upregulated in the Agg biofilm belonged to CC as assessed using GO analyses Specifically, several cell wall genes were upregulated in the Agg phenotype e.g., TSA1 , ECM33 , MP65 , ALS1 and PHR1 . In C. albicans , ECM33 and MP65 have been implemented as important proteins in maintenance of fungal cell wall integrity, biofilm formation and stress responses [41–43]. Furthermore, the ALS family of adhesin proteins and PHR family of extracellular transglycosylases also function as important regulators of biofilm formation in C. albicans [44, 45].…”

Section: Discussionmentioning

confidence: 99%

Candida aurisphenotypic heterogeneity determines pathogenicityin vitro

Brown

Delaney

Short

et al. 2020

Preprint

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1Candida auris is an enigmatic yeast that provides substantial global risk in 2 healthcare facilities and intensive care units. A unique phenotype exhibited by 3 certain isolates of C. auris is their ability to form small clusters of cells known as 4 aggregates, which have been to a limited extent described in the context of 5 pathogenic traits. In this study, we screened several non-aggregative and 6 aggregative C. auris isolates for biofilm formation, where we observed a level of 7 heterogeneity amongst the different phenotypes. Next, we utilised an RNA-8 sequencing approach to investigate the transcriptional responses during biofilm 9 formation of a non-aggregative and aggregative isolate of the initial pool. 10 Observations from these analyses indicate unique transcriptional profiles in the two 11 isolates, with several genes identified relating to proteins involved in adhesion and 12 invasion of the host in other fungal species. From these findings we investigated for 13 the first time the fungal recognition and inflammatory responses of a three-14 dimensional skin epithelial model to these isolates. In these models, a wound was 15 induced to mimic a portal of entry for C. auris. We show both phenotypes elicited 16 minimal response in the model minus induction of the wound, yet in the wounded 17 tissue both phenotypes induced a greater response, with the aggregative isolate 18 more pro-inflammatory. This capacity of aggregative C. auris biofilms to generate 19 such responses in the wounded skin highlights how this opportunistic yeast is a high 20 risk within the intensive care environment where susceptible patients have multiple 21 indwelling lines.22 23

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“…Primer3 software was used to design ERG3 primers. Primers for MP65 and ACT1 were obtained from the study of Sandini et al (Table 1) [8]. The actin producing gene, ACT1 was employed in the current investigation as the housekeeping gene.…”

Section: Real-time Pcrmentioning

confidence: 99%

“…Changes in MP65 gene which encodes a cell wall mannoprotein by tunicamycin also might affect cell wall organization and has been suggested as an attractive potential target for the development of antifungal drugs [8]. Previous studies indicate that MP65 gene appears in cell wall mannoprotein synthesis (Mp65p) and stability [8] which is then translated into fungal virulence.…”

Section: Introductionmentioning

confidence: 99%

Synergism effect of tunicamycin and amphotericin B causes suppression to the MP65 and ERG3 gene in oral associated-Candida albicans and C. dubliniensis

Omran

Harun

2016

Biotechnology & Biotechnological Equipment

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Synergism effect of tunicamycin and amphotericin B causes suppression to the MP65 and ERG3 gene in oral associated-Candida albicans and C. dubliniensis ABSTRACTCombination therapy can be used for the treatment of fungal infections, especially for those caused by antifungal-resistant fungi. In the present study, in vitro interactions and mechanisms between tunicamycin (TM) and amphotericin B (AmB) against Candida spp. were evaluated. The nature of the interactions determined by spectrophotometric method in a checkerboard assay was interpreted using nonparametric models of fractional inhibitory concentration index (FICI). We also evaluated the potential activity of TM in association with AmB on selected virulence gene (MP65 and ERG3) in C. albicans and C. dubliniensis. It was found that TM can work synergistically with AmB against C. dubliniensis; the minimum inhibitory concentration of TM and AmB decreased about fourfold, with an FICI of 0.5. The effect of combination has also significantly decreased the expression level of MP65 and ERG3 in which these genes have long been recognized as virulence traits in the pathogenicity of Candida (P ˂ 0.05). Our results suggest that the combination has the ability to reduce the growth of Candida in vitro.

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The MP65 gene is required for cell wall integrity, adherence to epithelial cells and biofilm formation in Candida albicans

Cited by 55 publications

References 53 publications

Candida albicans Biofilm Development and Its Genetic Control

Candida albicans Biofilm Development and Its Genetic Control

Candida aurisphenotypic heterogeneity determines pathogenicityin vitro

Synergism effect of tunicamycin and amphotericin B causes suppression to the MP65 and ERG3 gene in oral associated-Candida albicans and C. dubliniensis

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