The mouse SWISS-2D PAGE database: a tool for proteomics study of diabetes and obesity

Abstract

A number of two-dimensional electrophoresis (2-DE) reference maps from mouse samples have been established and could be accessed through the internet. An up-to-date list can be found in WORLD-2D PAGE (http://www.expasy.ch/ch2d/2d- index.html), an index of 2-DE databases and services. None of them were established from mouse white and brown adipose tissues, pancreatic islets, liver nuclei and skeletal muscle. This publication describes the mouse SWISS-2D PAGE database. Proteins present in samples of mouse (C57BI/6J) liver, liver nuclei, muscle, white and brown adipose tissue and pancreatic islets are assembled and described in an accessible uniform format. SWISS-2D PAGE can be accessed through the World Wide Web (WWW) network on the ExPASy molecular biology server (http://www.expasy.ch/ ch2d/).

Proteomic profiling of chronic low‐frequency stimulated fast muscle

“…Due to its more heterogeneous nature, the proteomic profiling of skeletal muscle fibres is still in its infancy, but has already made sub-stantial contributions to our general understanding of basic and applied myology. Recent studies have successfully cataloged the skeletal muscle proteome from various animal species [9][10][11][12][13][14], determined global differences in protein expression between predominantly slow-versus fast-twitching fibres [15][16][17][18], and have identified novel marker proteins of muscle growth [19], myoblast differentiation [20], neonatal fibre necrosis [18], hypertrophy [21], muscular dystrophy [22][23][24][25][26], dysferlinopathy [27], immobilization-induced atrophy [28,29] and ageing-induced sarcopenia [30][31][32][33][34]. In analogy, based on the findings of an initial proteomic analysis of the fast-to-slow fibre transformation process using a conventional nonfluorescent method [35], this report describes the detailed DIGE analysis of the differential expression of the fast skeletal muscle proteome following chronic low-frequency stimulation.…”

“…It is therefore essential to identify reliable skeletal muscle protein markers of fast-toslow muscle transformation, which might be helpful in establishing new molecular indicators of physiological and pathological fibre alterations. Building on previously established 2D skeletal muscle reference maps [10,11], we carried out a comprehensive proteomic profiling of the chronic lowfrequency stimulated rabbit tibialis anterior muscle and determined changes in the expression pattern of key muscle protein species. DIGE analysis and ESI-MS/MS for protein identification and immunoblotting clearly confirmed established changes in representative protein species from distinct functional muscle protein groupings, and uncovered interesting new transformation biomarkers.…”

“…The 2-D spot pattern of silver-stained gels was shown to be very comparable to published studies on the position of 2-D landmark species, such as contractile proteins [9][10][11][12][13][14][15][16][17][18], but the stimulated specimens differed considerably to the untreated control (Figs. 1A, C and E).…”

Fatty Acid Binding Protein as a Serum Marker for the Early Diagnosis of Stroke

“…The whole final diluted CSF sample corresponding to 45 g was loaded in a cup at the cathodic end of the immobilized pH gradient (IPG) strips. 2-DE was performed as described previously (10). In brief, the first dimensional protein separation was performed using a commercial 18-cm nonlinear IPG going from pH 3.5 to 10 from Amersham Biosciences (Uppsala, Sweden).…”

The application of proteomics to diabetes

“…The human pancreas has been explored taking a proteomics approach and a reference map of 302 proteins has been identified. 24 The same has been done in mouse islets, 25 and both of these will provide a useful reference for future proteomics studies of the pancreas in diabetes. Cytokines released from macrophages are known to be toxic to islet β cells, and this is thought to be one of the mechanisms involved in the development of type 1 DM in genetically predisposed individuals.…”