The mouse small ubiquitin-like modifier-2 (SUMO-2) inhibits interleukin-12 (IL-12) production in mature dendritic cells by blocking the translocation of the p65 subunit of NFκB into the nucleus

Kim, Eun Mi; Lee, Han Hyoung; Kim, Sang Hoon; Son, Young‐Ok; Lee, Suk Jun; Han, Ji‐Hye; Bae, Joonbeom; Kim, Sang Joon; Park, Chung Gyu; Park, Yong Soo; Hwang, Kwang Woo; Chun, Taehoon

doi:10.1016/j.molimm.2011.05.002

Cited by 19 publications

(19 citation statements)

References 34 publications

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“…Recently, Liu et al speculated that p65 and SUMO-2/3 interaction represents a novel mechanism by which p65 protein stability is regulated suppressing its degradation through ubiquitin-proteasome system in the cytoplasm (57). In details, p65 SUMOylation could be mediated by SUMO proteins that recruit an inhibitor of p65 in nucleus may be via SUMO-2/3 that has been described to inhibit nuclear import of p65 (58). Subsequentially, SUMO-2 or SUMO-3 induced the cytoplasmic level of p65 and may suppress its transcriptional activity (57,59).…”

Section: P65mentioning

confidence: 99%

SUMOylation and phosphorylation cross-talk in hepatocellular carcinoma

Tomasi

Ramani

2018

Transl. Gastroenterol. Hepatol.

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Section: P65mentioning

confidence: 99%

SUMOylation and phosphorylation cross-talk in hepatocellular carcinoma

Tomasi

Ramani

2018

Transl. Gastroenterol. Hepatol.

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“…The cDNA encoding ORF3-HA-expressing vector was constructed by inserting PCV2 ORF3 cDNA into the pLNCX2-HA vector. The cDNA encoding each expression vector was introduced into PK15 cells as previously described (Kim et al, 2011).…”

Section: Methodsmentioning

confidence: 99%

“…Confocal microscopy, Western blot analyses, co-IP and endogenous ubiquitination assays were performed as described previously (Kim et al, 2011). To measure stability of RGS16 protein, cells were treated or not treated with 100 mg ml 21 CHX (Sigma) for 6, 12, 18 and 24 h to measure RGS16 stability.…”

Section: Quantification Of the Interaction Between Rgs16 And Pcv2mentioning

confidence: 99%

The ORF3 protein of porcine circovirus type 2 promotes secretion of IL-6 and IL-8 in porcine epithelial cells by facilitating proteasomal degradation of regulator of G protein signalling 16 through physical interaction

Choi

Rho

Kim

et al. 2015

Journal of General Virology

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Porcine circovirus type 2 (PCV2) is the main aetiological agent of postweaning multisystemic wasting syndrome. The mechanism of pathogenicity associated with PCV2 infection is still not fully understood. Nevertheless, the fact that large amounts of proinflammatory cytokines within lymphoid tissues are released during the early stage of PCV2 infection may induce chronic inflammatory responses followed by the destruction of lymphoid tissues. However, how PCV2 infection causes an excessive inflammatory response in the host immune system during the early stage of PCV2 infection has still not been elucidated. In this study, we show that direct interaction between the PCV2 ORF3 and regulator of G protein signalling 16 (RGS16) within the cytoplasm of host cells leads to ubiquitin-mediated proteasomal degradation of RGS16. Facilitated degradation of the RGS16 by PCV2 ORF3 further enhances NFkB translocation into the nucleus through the ERK1/2 signalling pathway and increased IL-6 and IL-8 mRNA transcripts. Consequently, more severe inflammatory responses and leukocyte infiltration occur around host cells. This evidence may be the first clue explaining the molecular basis of how excessive amounts of proinflammatory cytokines within lymphoid tissues are released during the early stage of PCV2 infection.

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“…RT-PCR analyses were conducted as described previously (Kim et al 2011). Briefly, total RNAs from cells were isolated using a Trizol reagent (Invitrogen) according to the manufacture's protocol and reverse transcribed into cDNA using the First-Strand cDNA Synthesis Kit with oligo-dT primers (SuperScript RT; Invitrogen).…”

Section: Rt-pcr Analysesmentioning

confidence: 99%

“…Immunoreactive bands were visualized using an ECL system (GE Healthcare). An anti-b-actin antibody was utilized as an internal control (Kim et al 2011). …”

Section: Western Blot Analysesmentioning

confidence: 99%

Porcine testicular extract inhibits T cell proliferation by blocking cell cycle transition from G1 phase to S phase

Lee

Kim

et al. 2012

Biotechnol Lett

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Since T cells express diverse sex steroid hormone receptors, they might be a good model to evaluate the effects of sex steroid hormones on immune modulation. Porcine testicular extract contains several sex steroid hormones and may be useful to study the effects of sex steroid hormones during T cell activation. We have examined the effects of the porcine testicular extract on T cell activation: proliferation and secretion of cytokines (IL-2 and IFN-γ) by activated T cells were severely decreased after treatment with porcine testicular extract. The extract produced an immunosuppressive effect and inhibited the proliferation of activated T cells by blocking the cell cycle transition from the G(1) phase to S phase. These effects were mediated by a decrease in the expression of cyclin D1 and cyclin E and constitutive expression of p27(KIP1) after T cell activation.

show abstract

The mouse small ubiquitin-like modifier-2 (SUMO-2) inhibits interleukin-12 (IL-12) production in mature dendritic cells by blocking the translocation of the p65 subunit of NFκB into the nucleus

Cited by 19 publications

References 34 publications

SUMOylation and phosphorylation cross-talk in hepatocellular carcinoma

SUMOylation and phosphorylation cross-talk in hepatocellular carcinoma

The ORF3 protein of porcine circovirus type 2 promotes secretion of IL-6 and IL-8 in porcine epithelial cells by facilitating proteasomal degradation of regulator of G protein signalling 16 through physical interaction

Porcine testicular extract inhibits T cell proliferation by blocking cell cycle transition from G1 phase to S phase

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