The mouse intron-nested gene, Israa, is expressed in the lymphoid organs and involved in T-cell activation and signaling

Khalaf, Noureddine Ben; Al-Mashoor, Wedad; Saeed, Azhar; Al-Mehatab, Dalal; Taha, Safa; Bakhiet, Moiz; Fathallah, M. Dahmani

doi:10.1016/j.molimm.2019.04.006

Cited by 6 publications

(13 citation statements)

References 38 publications

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“…The immune mediator ISRAA has been well identified as an immune modulator, which mediates signals between the nervous and immune systems (1,2,5). The IFN- cytokine is a major effector molecule in the immune system, and it has been shown to play a key role in regulating brain growth (10,15) It is certain that this activity was not solely dependent on one mediator; it involved a network of factors.…”

Section: Discussionmentioning

confidence: 99%

“…Down regulation of T-cell activation and cytokine production has also been noted in the splenocytes of ISRAA knockout (-/-) mice, which show lymphoid organ hyperplasia. Furthermore, the transcription factor ELF1, which is involved in T cell regulation, is a binding partner for ISRAA, and a transcriptomic study using the ISRAA-overexpressing cell line EL4 has demonstrated the differential expression of numerous genes required for T-cell activation and growth (5).…”

Section: Introductionmentioning

confidence: 99%

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A role for the immune system-released activating agent (ISRAA) in the ontogenetic development of brain astrocytes

Al-Awadi

AlJawder

Mousa

et al. 2021

Preprint

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The Immune System-Released Activating Agent (ISRAA) was discovered as a novel molecule that functions as a mediator between the nervous and immune systems in response to a nervous stimulus following an immune challenge. This research investigated the role of ISRAA) in promoting the ontogeny of the mouse brain astrocytes. Astrocyte cultures were prepared from two-month-old BALB/c mice. Recombinant ISRAA protein was used to stimulate astrocyte cultures. Immunohistochemistry and ELISA were utilized to measure ISRAA and IFN-γ levels, IFN-γR expression and STAT1 nuclear translocation. MTT-assay was used to evaluate cellular survival and proliferation. To assess astrocyte cell lysates and tyrosine-phosphorylated proteins, SDS-PAGE and western blot were used. ISRAA was highly expressed in mouse embryonic astrocytes, depending on cell age. Astrocytes aged seven days (E7) showed increased proliferation and diminished differentiation, while 21-day-old (E21) astrocytes depicted reversed effects. ISRAA stimulated the tyrosine phosphorylation of numerous cellular proteins and the nuclear translocation of STAT1. IFN-γ was involved in the ISRAA action as ISRAA induced IFN-γ in both age groups, but only E21 astrocytes expressed IFN-γR. The results suggest that ISRAA is involved in mouse brain development through the cytokine network involving IFN-γ.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A role for the immune system-released activating agent (ISRAA) in the ontogenetic development of brain astrocytes

Al-Awadi

AlJawder

Mousa

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

“…The immune mediator ISRAA has been well identified as an immune modulator, which mediates signals between the nervous and immune systems [1,2,5]. The IFN-γ cytokine is a major effector molecule in the immune system, and it has been shown to play a key role in regulating brain growth [10,15].…”

Section: Discussionmentioning

confidence: 99%

“…Down regulation of T-cell activation and cytokine production has also been noted in the splenocytes of ISRAA knockout (-/-) mice, which show lymphoid organ hyperplasia. Furthermore, the transcription factor ELF1, which is involved in T cell regulation, is a binding partner for ISRAA, and a transcriptomic study using the ISRAAoverexpressing cell line EL4 has demonstrated the differential expression of numerous genes required for T-cell activation and growth [5].…”

Section: Introductionmentioning

confidence: 99%

A role for the immune system-released activating agent (ISRAA) in the ontogenetic development of brain astrocytes

et al. 2021

Self Cite

View full text Add to dashboard Cite

The Immune System-Released Activating Agent (ISRAA) was discovered as a novel molecule that functions as a mediator between the nervous and immune systems in response to a nervous stimulus following an immune challenge. This research investigated the role of ISRAA) in promoting the ontogeny of the mouse brain astrocytes. Astrocyte cultures were prepared from two-month-old BALB/c mice. Recombinant ISRAA protein was used to stimulate astrocyte cultures. Immunohistochemistry and ELISA were utilized to measure ISRAA and IFN-γ levels, IFN-γR expression and STAT1 nuclear translocation. MTT-assay was used to evaluate cellular survival and proliferation. To assess astrocyte cell lysates and tyrosine-phosphorylated proteins, SDS-PAGE and western blot were used. ISRAA was highly expressed in mouse embryonic astrocytes, depending on cell age. Astrocytes aged seven days (E7) showed increased proliferation and diminished differentiation, while 21-day-old (E21) astrocytes depicted reversed effects. IFN-γ was involved in the ISRAA action as ISRAA induced IFN-γ in both age groups, but only E21 astrocytes expressed IFN-γR. ISRAA stimulation of E21 resulted in tyrosine phosphorylation of numerous cellular proteins and the nuclear translocation of STAT1, a signalling pathway utilized by IFN-γ. The results suggest that ISRAA is involved in mouse brain development through the cytokine network involving IFN-γ.

show abstract

Section: Discussionmentioning

confidence: 99%

A Critical Role for Immune System-released Activating Agent (Israa) in the Ontogenetic Development of the Brain

Al-Awadi

AlJawder

Mousa

et al. 2020

Preprint

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Background: The Immune System-Released Activating Agent (ISRAA) was discovered as a novel molecule that functions as a mediator between the nervous and immune systems in response to a nervous stimulus following an immune challenge. Thisresearch investigated the role ofISRAA)in promoting the ontogeny of the mouse brain. Methods: Astrocyte cultures were prepared from two-month-old BALB/c mice. Recombinant ISRAA protein was used to stimulate astrocyte cultures. Immunohistochemistry and ELISA were utilized to measure ISRAA andIFN-g levels, IFN-gR expression and STAT1 nuclear translocation. MTT-assay was used to evaluate cellular survival and proliferation. To assess astrocyte cell lysates and tyrosine-phosphorylated proteins, SDS-PAGE and western blot were used.Results: ISRAA was highly expressed in mouse embryonic astrocytes,depending on cell age. Astrocytes agedseven days (E7) showed increased proliferation and diminisheddifferentiation, while 21-day-old (E21)astrocytes depictedreversed effects. ISRAAstimulated the tyrosine phosphorylation of numerous cellular proteins and thenuclear translocation of STAT1. IFN-g was vital for ISRAAactionas ISRAA induced IFN-gin both age groups, but only E21 astrocytes expressed IFN-gR. Conclusion: The results suggest that ISRAA is cruciallyinvolved in mouse brain development through the cytokine network involving IFN-g.

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The mouse intron-nested gene, Israa, is expressed in the lymphoid organs and involved in T-cell activation and signaling

Cited by 6 publications

References 38 publications

A role for the immune system-released activating agent (ISRAA) in the ontogenetic development of brain astrocytes

A role for the immune system-released activating agent (ISRAA) in the ontogenetic development of brain astrocytes

A role for the immune system-released activating agent (ISRAA) in the ontogenetic development of brain astrocytes

A Critical Role for Immune System-released Activating Agent (Israa) in the Ontogenetic Development of the Brain

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