The mouse excisional wound splinting model, including applications for stem cell transplantation

Wang, Xusheng; Ge, Jianfeng; Tredget, Edward E.; Wu, Yaojiong

doi:10.1038/nprot.2013.002

Cited by 334 publications

(313 citation statements)

References 23 publications

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“…This rate is comparable to the data given in the literature where 50-90% wound contraction was seen in excisional wound in rats, depending on wound geometry, anatomical location and the presence or absence of panniculus carnosus [53,54]. There are models to limit wound contraction to a degree comparable to that of humans (25-40% [55]), including splints secured to the skin with adhesives [56]. However, splinting has been shown to alter wound healing, probably by mechanical and biochemical signaling in the wound, and it is not yet clear, whether the healing process induced by these models is actually closer to that of humans [57].…”

Section: Survival Time -7 Dayssupporting

confidence: 72%

Biocompatibility of Pegylated Fibrinogen and Its Effect on Healing of Full-Thickness Skin Defects: A Preliminary Study in Rats

CM¹,

Jacot²

2016

J Biotechnol Biomater

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Citation: Venzin CM, Jacot V, Berdichevsky A, Karol AA, Seliktar D, et al. (2016) AbstractIntroduction: A synthetic polymer polyethylene glycol (PEG), was conjugated to fibrinogen as a threedimensional and biodegradable skin wound dressing matrix. This PEG-fibrinogen (PEG-fib) was tested in vivo in a skin wound time course study for its biocompatibility and biodegradation, after being delivered into the wound by injection and polymerized in situ by photo-activation.

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Section: Survival Time -7 Dayssupporting

confidence: 72%

Biocompatibility of Pegylated Fibrinogen and Its Effect on Healing of Full-Thickness Skin Defects: A Preliminary Study in Rats

CM¹,

Jacot²

2016

J Biotechnol Biomater

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“…Macroscopic analysis of the wound area mainly detects differences in wound contraction, a process that is particularly efficient in rodent wounds (25). To detect potential differences in granulation tissue formation or reepithelialization, we performed histomorphometrical analysis of sections from the middle of the wounds at day 5 after injury ( Fig.…”

Section: Wound Closure Is Not Impaired In Mast Cell-deficient Micementioning

confidence: 99%

Mast Cells Are Dispensable for Normal and Activin-Promoted Wound Healing and Skin Carcinogenesis

Antsiferova

Martin

Huber

et al. 2013

The Journal of Immunology

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The growth and differentiation factor activin A is a key regulator of tissue repair, inflammation, fibrosis, and tumorigenesis. However, the cellular targets, which mediate the different activin functions, are still largely unknown. In this study, we show that activin increases the number of mature mast cells in mouse skin in vivo. To determine the relevance of this finding for wound healing and skin carcinogenesis, we mated activin transgenic mice with CreMaster mice, which are characterized by Cre recombinase-mediated mast cell eradication. Using single- and double-mutant mice, we show that loss of mast cells neither affected the stimulatory effect of overexpressed activin on granulation tissue formation and reepithelialization of skin wounds nor its protumorigenic activity in a model of chemically induced skin carcinogenesis. Furthermore, mast cell deficiency did not alter wounding-induced inflammation and new tissue formation or chemically induced angiogenesis and tumorigenesis in mice with normal activin levels. These findings reveal that mast cells are not major targets of activin during wound healing and skin cancer development and also argue against nonredundant functions of mast cells in wound healing and skin carcinogenesis in general.

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“…Experiments with animals were carried out under approval by the Institutional Animal Care and Use Committee of Seoul National University (SNU-150508-4) and in accordance with the NIH Guide for the Care and Use of Laboratory Animals. Methods for the mouse skin excision wound splinting model and stem cell transplantation were performed according to a previous study [23]. Mice were randomly divided into groups (n = 5, each group).…”

Section: Mouse Skin Wound Healing Modelmentioning

confidence: 99%

cAMP Promotes Cell Migration Through Cell Junctional Complex Dynamics and Actin Cytoskeleton Remodeling: Implications in Skin Wound Healing

Kim

Ryu

Park

et al. 2015

Stem Cells and Development

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Stem cells have attracted great interest for their therapeutic capacity in tissue regeneration. Cyclic adenosine 3¢,5¢-monophosphate (cAMP), existing in high concentration at wound sites, mediated various signaling pathways such as cytoskeleton dynamics, cell adhesion, and cell migration in stem cells, which suggest the critical roles of cAMP in the wound healing process through functional regulation of stem cells. However, the mechanisms behind the effect of cAMP on mouse embryonic stem cell (mESC) motility and its roles on skin wound healing remain to be fully elucidated. In the present study, 8-Bromo cAMP-treated mESCs showed significant wound closure and improved neovascularization. Moreover, 8-Bromo cAMP stimulated mESC migration into the wound bed. 8-Bromo cAMP also increased ESC motility in in vitro migration assay. 8-Bromo cAMP induced myosin light chain phosphorylation through Rac1 and Cdc42 signaling, which were involved in 8-Bromo cAMP-induced decrease in expression of junction proteins (connexin 43, E-cadherin, and occludin) at the plasma membrane. Subsequently, 8-Bromo cAMP induced the disruption of cell junctions (including gap junctions, adherens junctions, and tight junctions), which reduced the function of the gap junctions and cell adhesion. In addition, 8-Bromo cAMP-induced Rac1 and Cdc42 activation increased Arp3, TOCA, PAK, and N-WASP expression, but decreased cofilin phosphorylation level, which elicited actin cytoskeleton remodeling. In contrast to the control, 8-Bromo cAMP evoked a substantial migration of cells into the denuded area, which was blocked by the small interfering RNAs of the signaling pathway-related molecules or by inhibitors. In conclusion, cAMP enhanced the migration of mESCs through effective coordination of junctional disruption and actin cytoskeleton remodeling, which increased the wound healing capacity of ESCs.

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The mouse excisional wound splinting model, including applications for stem cell transplantation

Cited by 334 publications

References 23 publications

Biocompatibility of Pegylated Fibrinogen and Its Effect on Healing of Full-Thickness Skin Defects: A Preliminary Study in Rats

Biocompatibility of Pegylated Fibrinogen and Its Effect on Healing of Full-Thickness Skin Defects: A Preliminary Study in Rats

Mast Cells Are Dispensable for Normal and Activin-Promoted Wound Healing and Skin Carcinogenesis

cAMP Promotes Cell Migration Through Cell Junctional Complex Dynamics and Actin Cytoskeleton Remodeling: Implications in Skin Wound Healing

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